LOCUS LT727024 5160 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(444-560), complete
sequence.
ACCESSION LT727024
VERSION LT727024.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(444-560)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(444-560)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5160)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5160
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(444-560)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5370."
/db_xref="taxon:1944795"
misc_feature 16..54
/note="E-tag"
CDS <64..417
/codon_start=1
/transl_table=11
/note="mABIN-1(444-560)"
/protein_id="SJL87221.1"
/translation="ASPSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQ
RERSDRERMNEEKEELKKQVEKLQAQVTLTNAQLKTLKEEEKAKEALKQQKRKAKASG
ERYHMEPHPEHVCGA"
regulatory 450..541
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 542..898
/note="3' FR of beta-globin"
regulatory complement(976..1058)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1200..1275
/note="SV40 ori"
regulatory complement(1306..1380)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1306..1380
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1495..1896)
/note="pMB1 ori"
CDS complement(2437..3297)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87222.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3433..3810
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3816..5157
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4185..5109
/note="intron AG promoter"
BASE COUNT 1112 a 1368 c 1445 g 1235 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagcctctc catcatctcc gcctgcagct tttgggagtc cagaaggcgt tgggggccat
121 ctgaggaagc aggaactagt gacacagaat gagttgctga aacagcaggt aaagatcttt
181 gaagaggact tccagaggga acggagtgac cgtgaacgca tgaatgaaga gaaggaggag
241 ctgaagaagc aagtagagaa gctgcaggcc caggtcaccc tgactaatgc ccagctcaaa
301 actctcaaag aggaggagaa ggccaaggaa gccctcaaac agcagaagag gaaagcaaag
361 gcttcgggag agcgctacca catggaaccc caccctgagc acgtctgcgg cgcctgaccc
421 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
481 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
541 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
601 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
661 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
721 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
781 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
841 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
901 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg
1021 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
1081 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
1141 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
1201 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
1261 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
1321 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
1381 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
1441 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
1501 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
1561 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat
1621 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
1681 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
1741 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
1801 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
1861 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
1921 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
1981 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
2041 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2101 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2161 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2221 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2281 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2341 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2401 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
2461 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
2521 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
2581 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
2641 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
2701 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
2761 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
2821 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
2881 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
2941 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
3001 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
3061 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3121 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3181 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
3241 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
3301 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
3361 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
3421 gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
3481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
3541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
3601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
3661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
3721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
3781 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
3841 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
3901 tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg
3961 gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc
4021 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
4081 cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct
4141 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
4201 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
4261 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
4321 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
4381 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
4441 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
4501 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
4561 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
4621 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
4681 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc
4741 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
4801 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
4861 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
4921 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
4981 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
5041 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
5101 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//