LOCUS LT727022 5593 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(444-647), complete
sequence.
ACCESSION LT727022
VERSION LT727022.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(444-647)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(444-647)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5593)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5593
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(444-647)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5369."
/db_xref="taxon:1944797"
misc_feature 16..54
/note="E-tag"
CDS <64..678
/codon_start=1
/transl_table=11
/note="mABIN-1 (444-647)"
/protein_id="SJL87217.1"
/translation="ASPSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQ
RERSDRERMNEEKEELKKQVEKLQAQVTLTNAQLKTLKEEEKAKEALKQQKRKAKASG
ERYHMEPHPEHVCGAYPYAYPPMPAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNSRL
FHLPEYTWRPPCAGIRNQSSQVMDPPPDRPAEPESADNDCDGPQ"
regulatory 883..974
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 975..1331
/note="3' FR of beta-globin"
regulatory complement(1409..1491)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1633..1708
/note="SV40 ori"
regulatory 1739..1813
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1739..1813)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1928..2329)
/note="pMB1 ori"
CDS complement(2870..3730)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87218.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3866..4243
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4249..5590
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4618..5542
/note="intron AG promoter"
BASE COUNT 1205 a 1516 c 1551 g 1321 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagcctctc catcatctcc gcctgcagct tttgggagtc cagaaggcgt tgggggccat
121 ctgaggaagc aggaactagt gacacagaat gagttgctga aacagcaggt aaagatcttt
181 gaagaggact tccagaggga acggagtgac cgtgaacgca tgaatgaaga gaaggaggag
241 ctgaagaagc aagtagagaa gctgcaggcc caggtcaccc tgactaatgc ccagctcaaa
301 actctcaaag aggaggagaa ggccaaggaa gccctcaaac agcagaagag gaaagcaaag
361 gcttcgggag agcgctacca catggaaccc caccctgagc acgtctgcgg cgcctatccc
421 tatgcctacc cacccatgcc agccatggta cctcaccatg cctacaagga ctggtcccag
481 atccgatacc ctccaccccc tgtgcccatg gagcacccgc ccccacaccc caactctcgc
541 ctcttccatc tgccggagta cacctggcgt ccaccctgtg cagggattcg gaatcagagc
601 tctcaagtga tggacccgcc cccagacagg cctgcagagc cagagtctgc agacaatgac
661 tgtgatgggc cccagtgagg ctgcagtggg tcatttggtt ccaccttcat ctttcagagc
721 cagctgacct cagattgcca aaagtttgaa ggccatgtgc atgttctgtg tgacccaagc
781 cttggcagag gagaggctgg gatgggtagc tggctcacat ccccagccaa gcctcgaact
841 gttgacaaga cccccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
901 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
961 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
1021 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
1081 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
1141 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
1201 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
1261 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
1321 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
1381 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
1441 taaagcctgg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
1501 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
1561 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
1621 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
1681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
1741 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
1801 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
1861 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
1921 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
1981 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg
2041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
2101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
2161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
2221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
2281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
2341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
2401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
2461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
2521 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
2581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
2641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
2701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
2761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
3001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
3781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
3841 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca
3901 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
3961 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
4021 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
4081 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
4141 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
4201 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
4261 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
4321 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc
4381 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat
4441 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
4501 aaaaagcgaa gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc
4561 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
4621 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
4681 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
4741 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
4801 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
4861 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
4921 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
4981 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
5041 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
5101 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg
5161 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
5221 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
5281 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
5341 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
5401 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
5461 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
5521 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
5581 ttttggcaaa gaa
//