LOCUS LT727021 5355 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(420-601), complete
sequence.
ACCESSION LT727021
VERSION LT727021.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(420-601)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(420-601)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5355)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5355
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(420-601)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5368."
/db_xref="taxon:1944792"
misc_feature 16..54
/note="E-tag"
CDS <64..612
/codon_start=1
/transl_table=11
/note="mABIN-1 (420-601)"
/protein_id="SJL87215.1"
/translation="RQREYQEKEIQRLNKALEEALSIQASPSSPPAAFGSPEGVGGHL
RKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLTNAQL
KTLKEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGAYPYAYPPMPAMVPHHAYKD
WSQIRYPPPPVPMEHPPPHPNS"
regulatory 645..736
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 737..1093
/note="3' FR of beta-globin"
regulatory complement(1171..1253)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1395..1470
/note="SV40 ori"
regulatory complement(1501..1575)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1501..1575
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1690..2091)
/note="pMB1 ori"
CDS complement(2632..3492)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87216.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3628..4005
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4011..5352
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4380..5304
/note="intron AG promoter"
BASE COUNT 1161 a 1445 c 1486 g 1263 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcaaggcaac gagaatacca ggagaaggag atccagcggc tcaataaggc cctggaggag
121 gccctcagca tccaggcctc tccatcatct ccgcctgcag cttttgggag tccagaaggc
181 gttgggggcc atctgaggaa gcaggaacta gtgacacaga atgagttgct gaaacagcag
241 gtaaagatct ttgaagagga cttccagagg gaacggagtg accgtgaacg catgaatgaa
301 gagaaggagg agctgaagaa gcaagtagag aagctgcagg cccaggtcac cctgactaat
361 gcccagctca aaactctcaa agaggaggag aaggccaagg aagccctcaa acagcagaag
421 aggaaagcaa aggcttcggg agagcgctac cacatggaac cccaccctga gcacgtctgc
481 ggcgcctatc cctatgccta cccacccatg ccagccatgg tacctcacca tgcctacaag
541 gactggtccc agatccgata ccctccaccc cctgtgccca tggagcaccc gcccccacac
601 cccaactctt gacccccaat gccctggctc acaaatacca ctgagatctt tttccctctg
661 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
721 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
781 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
841 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
901 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
961 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
1021 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
1081 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
1141 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
1201 tgtaaagcct gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
1261 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
1321 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
1381 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
1441 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
1501 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
1561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
1621 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
1681 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
1741 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa
1801 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
1861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
1921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
1981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
2041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
2101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
2161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
2221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
2281 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
2341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
2401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
2461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
2521 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
2581 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
2641 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
2701 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
2761 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
2821 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
2881 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
2941 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
3001 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
3061 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
3121 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
3181 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
3241 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
3301 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
3361 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
3421 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
3481 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
3541 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
3601 atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat
3661 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
3721 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
3781 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
3841 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
3901 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
3961 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
4021 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
4081 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag
4141 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca
4201 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
4261 ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc
4321 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
4381 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
4441 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
4501 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
4561 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
4621 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
4681 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
4741 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
4801 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
4861 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag
4921 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
4981 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
5041 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
5101 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
5161 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
5221 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
5281 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
5341 cattttggca aagaa
//