LOCUS LT727020 5232 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(420-560), complete
sequence.
ACCESSION LT727020
VERSION LT727020.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(420-560)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(420-560)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5232)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5232
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(420-560)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5367."
/db_xref="taxon:1944791"
misc_feature 16..54
/note="E-tag"
CDS <64..489
/codon_start=1
/transl_table=11
/note="mABIN-1(420-560)"
/protein_id="SJL87213.1"
/translation="RQREYQEKEIQRLNKALEEALSIQASPSSPPAAFGSPEGVGGHL
RKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLTNAQL
KTLKEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGA"
regulatory 522..613
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 614..970
/note="3' FR of beta-globin"
regulatory complement(1048..1130)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1272..1347
/note="SV40 ori"
regulatory complement(1378..1452)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1378..1452
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1567..1968)
/note="pMB1 ori"
CDS complement(2509..3369)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87214.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3505..3882
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3888..5229
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4257..5181
/note="intron AG promoter"
BASE COUNT 1135 a 1387 c 1468 g 1242 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcaaggcaac gagaatacca ggagaaggag atccagcggc tcaataaggc cctggaggag
121 gccctcagca tccaggcctc tccatcatct ccgcctgcag cttttgggag tccagaaggc
181 gttgggggcc atctgaggaa gcaggaacta gtgacacaga atgagttgct gaaacagcag
241 gtaaagatct ttgaagagga cttccagagg gaacggagtg accgtgaacg catgaatgaa
301 gagaaggagg agctgaagaa gcaagtagag aagctgcagg cccaggtcac cctgactaat
361 gcccagctca aaactctcaa agaggaggag aaggccaagg aagccctcaa acagcagaag
421 aggaaagcaa aggcttcggg agagcgctac cacatggaac cccaccctga gcacgtctgc
481 ggcgcctgac ccccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
541 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
601 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
661 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
721 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
781 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
841 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
901 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
961 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
1021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
1081 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
1141 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
1201 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
1261 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
1321 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
1381 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
1441 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
1501 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
1561 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
1621 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc
1681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
1741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
1801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
1861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
1921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
1981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
2041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
2101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
2161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
2221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
2281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
2341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
2401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
2461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
2521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
2581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
2641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
2701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
2761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
2821 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
2881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
2941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
3001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
3061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
3121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
3181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
3241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
3301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
3361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
3421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
3481 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa
3541 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
3601 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
3661 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
3721 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
3781 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
3841 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
3901 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
3961 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg
4021 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc
4081 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
4141 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct
4201 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
4261 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
4321 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
4381 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
4441 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
4501 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
4561 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
4621 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
4681 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
4741 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg
4801 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
4861 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
4921 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
4981 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
5041 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
5101 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
5161 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
5221 tttggcaaag aa
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