LOCUS LT727019 5451 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(388-601), complete
sequence.
ACCESSION LT727019
VERSION LT727019.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(388-601)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(388-601)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5451)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5451
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(388-601)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5366."
/db_xref="taxon:1944788"
misc_feature 16..54
/note="E-tag"
CDS <64..708
/codon_start=1
/transl_table=11
/note="mABIN-1 (388-601)"
/protein_id="SJL87211.1"
/translation="EMEETDKEQLTAEAKELRQKVRYLQDQLSPLTRQREYQEKEIQR
LNKALEEALSIQASPSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQRE
RSDRERMNEEKEELKKQVEKLQAQVTLTNAQLKTLKEEEKAKEALKQQKRKAKASGER
YHMEPHPEHVCGAYPYAYPPMPAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNS"
regulatory 741..832
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 833..1189
/note="3' FR of beta-globin"
regulatory complement(1267..1349)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1491..1566
/note="SV40 ori"
regulatory complement(1597..1671)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1597..1671
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1786..2187)
/note="pMB1 ori"
CDS complement(2728..3588)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87212.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3724..4101
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4107..5448
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4476..5400
/note="intron AG promoter"
BASE COUNT 1192 a 1470 c 1517 g 1272 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagagatgg aagagaccga caaggagcag ctgacagcag aggccaagga actgcgccag
121 aaggtcaggt acctacagga tcagctgagc ccgctcacaa ggcaacgaga ataccaggag
181 aaggagatcc agcggctcaa taaggccctg gaggaggccc tcagcatcca ggcctctcca
241 tcatctccgc ctgcagcttt tgggagtcca gaaggcgttg ggggccatct gaggaagcag
301 gaactagtga cacagaatga gttgctgaaa cagcaggtaa agatctttga agaggacttc
361 cagagggaac ggagtgaccg tgaacgcatg aatgaagaga aggaggagct gaagaagcaa
421 gtagagaagc tgcaggccca ggtcaccctg actaatgccc agctcaaaac tctcaaagag
481 gaggagaagg ccaaggaagc cctcaaacag cagaagagga aagcaaaggc ttcgggagag
541 cgctaccaca tggaacccca ccctgagcac gtctgcggcg cctatcccta tgcctaccca
601 cccatgccag ccatggtacc tcaccatgcc tacaaggact ggtcccagat ccgataccct
661 ccaccccctg tgcccatgga gcacccgccc ccacacccca actcttgacc cccaatgccc
721 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
781 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
841 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
901 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
961 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
1021 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
1081 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
1141 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
1201 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
1261 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag
1321 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
1381 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
1441 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
1501 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
1561 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
1621 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
1681 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
1741 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
1801 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
1861 aggcggtaat acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa
1921 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
1981 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
2041 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
2101 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
2161 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
2221 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
2281 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
2341 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
2401 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
2461 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
2521 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
2581 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
2641 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
2701 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
2761 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
2821 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
2881 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
2941 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
3001 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
3061 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
3121 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
3181 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
3241 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
3301 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
3361 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
3421 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
3481 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
3541 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
3601 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
3661 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
3721 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
3781 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
3841 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
3901 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
3961 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
4021 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
4081 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
4141 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
4201 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
4261 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
4321 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
4381 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
4441 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
4501 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
4561 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
4621 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
4681 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
4741 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
4801 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
4861 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
4921 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
4981 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
5041 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
5101 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
5161 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
5221 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
5281 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
5341 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
5401 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a
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