LOCUS LT727016 5350 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(525-647), complete
sequence.
ACCESSION LT727016
VERSION LT727016.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(525-647)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(525-647)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5350)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5350
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(525-647)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5362."
/db_xref="taxon:1944802"
misc_feature 16..54
/note="E-tag"
CDS <64..435
/codon_start=1
/transl_table=11
/note="mABIN-1(525-647)"
/protein_id="SJL87205.1"
/translation="KEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGAYPYAYPPM
PAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNSRLFHLPEYTWRPPCAGIRNQSSQVM
DPPPDRPAEPESADNDCDGPQ"
regulatory 640..731
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 732..1088
/note="3' FR of beta-globin"
regulatory complement(1166..1248)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1390..1465
/note="SV40 ori"
regulatory complement(1496..1570)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1496..1570
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1685..2086)
/note="pMB1 ori"
CDS complement(2627..3487)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87206.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3623..4000
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4006..5347
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4375..5299
/note="intron AG promoter"
BASE COUNT 1131 a 1462 c 1478 g 1279 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gccaaagagg aggagaaggc caaggaagcc ctcaaacagc agaagaggaa agcaaaggct
121 tcgggagagc gctaccacat ggaaccccac cctgagcacg tctgcggcgc ctatccctat
181 gcctacccac ccatgccagc catggtacct caccatgcct acaaggactg gtcccagatc
241 cgataccctc caccccctgt gcccatggag cacccgcccc cacaccccaa ctctcgcctc
301 ttccatctgc cggagtacac ctggcgtcca ccctgtgcag ggattcggaa tcagagctct
361 caagtgatgg acccgccccc agacaggcct gcagagccag agtctgcaga caatgactgt
421 gatgggcccc agtgaggctg cagtgggtca tttggttcca ccttcatctt tcagagccag
481 ctgacctcag attgccaaaa gtttgaaggc catgtgcatg ttctgtgtga cccaagcctt
541 ggcagaggag aggctgggat gggtagctgg ctcacatccc cagccaagcc tcgaactgtt
601 gacaagaccc ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
661 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
721 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
781 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
841 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
901 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
961 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
1021 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
1081 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
1141 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
1201 agcctgggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
1261 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
1321 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
1381 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
1441 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
1501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
1561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
1621 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
1681 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
1741 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag
1801 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
1861 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
1921 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
1981 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
2041 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
2101 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
2161 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
2221 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
2281 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
2341 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
2401 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
2461 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
2521 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
2581 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
2641 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
2701 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
2761 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
2821 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
2881 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
2941 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
3001 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
3061 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
3121 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
3181 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
3241 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
3301 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
3361 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
3421 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
3481 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
3541 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
3601 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt
3661 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
3721 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
3781 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
3841 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
3901 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
3961 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
4021 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
4081 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg
4141 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag
4201 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
4261 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc
4321 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
4381 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
4441 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
4501 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
4561 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
4621 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
4681 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
4741 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
4801 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
4861 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg
4921 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
4981 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
5041 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
5101 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
5161 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
5221 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
5281 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
5341 tggcaaagaa
//