LOCUS LT727015 5665 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(420-647), complete
sequence.
ACCESSION LT727015
VERSION LT727015.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(420-647)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(420-647)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5665)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5665
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(420-647)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5361."
/db_xref="taxon:1944793"
misc_feature 16..54
/note="E-tag"
CDS <64..750
/codon_start=1
/transl_table=11
/note="mABIN-1(420-647)"
/protein_id="SJL87203.1"
/translation="RQREYQEKEIQRLNKALEEALSIQASPSSPPAAFGSPEGVGGHL
RKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLTNAQL
KTLKEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGAYPYAYPPMPAMVPHHAYKD
WSQIRYPPPPVPMEHPPPHPNSRLFHLPEYTWRPPCAGIRNQSSQVMDPPPDRPAEPE
SADNDCDGPQ"
regulatory 955..1046
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1047..1403
/note="3' FR of beta-globin"
regulatory complement(1481..1563)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1705..1780
/note="SV40 ori"
regulatory complement(1811..1885)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1811..1885
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2000..2401)
/note="pMB1 ori"
CDS complement(2942..3802)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87204.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3938..4315
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4321..5662
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4690..5614
/note="intron AG promoter"
BASE COUNT 1228 a 1535 c 1574 g 1328 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcaaggcaac gagaatacca ggagaaggag atccagcggc tcaataaggc cctggaggag
121 gccctcagca tccaggcctc tccatcatct ccgcctgcag cttttgggag tccagaaggc
181 gttgggggcc atctgaggaa gcaggaacta gtgacacaga atgagttgct gaaacagcag
241 gtaaagatct ttgaagagga cttccagagg gaacggagtg accgtgaacg catgaatgaa
301 gagaaggagg agctgaagaa gcaagtagag aagctgcagg cccaggtcac cctgactaat
361 gcccagctca aaactctcaa agaggaggag aaggccaagg aagccctcaa acagcagaag
421 aggaaagcaa aggcttcggg agagcgctac cacatggaac cccaccctga gcacgtctgc
481 ggcgcctatc cctatgccta cccacccatg ccagccatgg tacctcacca tgcctacaag
541 gactggtccc agatccgata ccctccaccc cctgtgccca tggagcaccc gcccccacac
601 cccaactctc gcctcttcca tctgccggag tacacctggc gtccaccctg tgcagggatt
661 cggaatcaga gctctcaagt gatggacccg cccccagaca ggcctgcaga gccagagtct
721 gcagacaatg actgtgatgg gccccagtga ggctgcagtg ggtcatttgg ttccaccttc
781 atctttcaga gccagctgac ctcagattgc caaaagtttg aaggccatgt gcatgttctg
841 tgtgacccaa gccttggcag aggagaggct gggatgggta gctggctcac atccccagcc
901 aagcctcgaa ctgttgacaa gacccccaat gccctggctc acaaatacca ctgagatctt
961 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
1021 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
1081 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
1141 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
1201 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
1261 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
1321 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
1381 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
1441 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
1501 aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt aattgcgttg
1561 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
1621 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
1681 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
1741 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
1801 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
1861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
1921 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga
1981 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
2041 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
2101 tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
2161 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
2221 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
2281 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
2341 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
2401 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
2461 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
2521 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
2581 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
2641 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
2701 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
2761 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
2821 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
2881 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
2941 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
3001 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
3061 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
3121 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
3181 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
3241 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
3301 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
3361 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
3421 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
3481 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
3541 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
3601 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
3661 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
3721 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
3781 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
3841 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
3901 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat
3961 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
4021 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
4081 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
4141 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
4201 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
4261 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
4321 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
4381 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
4441 ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg
4501 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
4561 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc
4621 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
4681 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
4741 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
4801 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
4861 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
4921 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
4981 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
5041 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
5101 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
5161 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
5221 tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
5281 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
5341 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
5401 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
5461 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
5521 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
5581 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
5641 gctgtctcat cattttggca aagaa
//