LOCUS LT727014 5814 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(54-388), complete
sequence.
ACCESSION LT727014
VERSION LT727014.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(54-388)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(54-388)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5814)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5814
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(54-388)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5360."
/db_xref="taxon:1944803"
misc_feature 16..54
/note="E-tag"
CDS 64..1071
/transl_table=11
/note="mABIN-1(54-388)"
/protein_id="SJL87201.1"
/translation="MEASRLRQKAEELVKDSELSPPTSAPSLVSFDDLAELTGQDTKV
QVHPATSTAATTTATATTGNSMEKPEPASKSPSNGASSDFEVVPTEEQNSPETGSHPT
NMMDLGPPPPEDSNLKLHLQRLETTLSVCAEEPDHSQLFTHLGRMALEFNRLASKVHK
NEQRTSILQTLCEQLRQENEALKAKLDKGLEQRDLAAERLREENTELKKLLMNSSCKE
GLCGQPSSPKPEGAGKKGVAGQQQASVMASKVPEAGAFGAAEKKVKLLEQQRMELLEV
NKQWDQHFRSMKQQYEQKITELRQKLVDLQKQVTELEAEREQKQRDFDRKLLLAKSKI
E"
regulatory 1104..1195
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1196..1552
/note="3' FR of beta-globin"
regulatory complement(1630..1712)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1854..1929
/note="SV40 ori"
regulatory complement(1960..2034)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1960..2034
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2149..2550)
/note="pMB1 ori"
CDS complement(3091..3951)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87202.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4087..4464
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4470..5811
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4839..5763
/note="intron AG promoter"
BASE COUNT 1268 a 1575 c 1637 g 1334 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcgatggaag cgtccagact ccggcagaag gcagaggagc tggtcaagga cagcgagctg
121 tcaccaccga catctgcccc ctccttggtc tcctttgatg acctggctga gctcacagga
181 caggatacaa aggtccaggt acatcctgct accagcactg ccgccaccac caccgccacc
241 gccaccacgg gaaactccat ggagaagccc gagccagcct ccaaatctcc gtccaatggc
301 gcctcctcgg actttgaagt ggtccctact gaggagcaga attcacccga aactggcagc
361 caccctacga acatgatgga cctggggccc ccacccccag aggacagcaa cctgaagctc
421 cacctgcagc gcctggagac cacccttagc gtgtgtgcag aggagccaga ccacagccag
481 ctcttcaccc acctgggccg catggccctc gagttcaaca ggttggcctc caaagtgcat
541 aaaaatgagc agcgcacctc catcctgcag accttatgtg agcagctgcg ccaggagaat
601 gaagccctga aggccaagct ggacaagggc ctggaacagc gggatctggc tgctgagagg
661 ctgcgggagg aaaacacgga gctcaagaaa ctgttgatga acagcagctg caaagaggga
721 ctctgtgggc agcccagctc cccaaagcca gagggtgctg gcaagaaggg cgtggctgga
781 cagcagcagg ccagtgtgat ggcgagtaaa gtccctgaag cgggggcctt tggagcagct
841 gagaagaaag tgaagttgct agaacagcaa cgcatggagc tgctggaagt gaacaagcag
901 tgggaccagc atttccggtc catgaagcag cagtatgagc agaagatcac agagcttcgc
961 cagaagctgg tggacctgca gaaacaggta actgagctgg aggccgaacg ggagcagaag
1021 cagcgtgact ttgaccggaa actcctcctg gccaaatcga agatagagtg acccccaatg
1081 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
1141 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
1201 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
1261 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
1321 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
1381 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
1441 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
1501 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
1561 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
1621 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg ggtgcctaat
1681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
1741 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
1801 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
1861 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
1921 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
1981 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
2041 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
2101 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
2161 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
2221 caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc
2281 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
2341 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
2401 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
2461 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
2521 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
2581 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
2641 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
2701 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
2761 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
2821 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
2881 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
2941 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
3001 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
3061 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
3121 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
3181 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
3241 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
3301 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
3361 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
3421 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
3481 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
3541 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
3601 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
3661 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
3721 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
3781 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
3841 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
3901 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
3961 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
4021 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
4081 tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
4141 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
4201 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
4261 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
4321 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
4381 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
4441 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
4501 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
4561 cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg cggggcgagg
4621 ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
4681 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
4741 gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc gcctcgcgcc
4801 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
4861 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
4921 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
4981 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
5041 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
5101 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
5161 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
5221 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
5281 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
5341 gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg cccccggagc
5401 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
5461 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
5521 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
5581 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
5641 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
5701 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
5761 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaa
//