LOCUS LT727013 5761 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(390-647), complete
sequence.
ACCESSION LT727013
VERSION LT727013.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(390-647)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(390-647)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5761)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5761
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(390-647)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5358."
/db_xref="taxon:1944789"
misc_feature 16..54
/note="E-tag"
CDS 67..846
/transl_table=11
/note="mABIN-1(390-647)"
/protein_id="SJL87199.1"
/translation="MEETDKEQLTAEAKELRQKVRYLQDQLSPLTRQREYQEKEIQRL
NKALEEALSIQASPSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQRER
SDRERMNEEKEELKKQVEKLQAQVTLTNAQLKTLKEEEKAKEALKQQKRKAKASGERY
HMEPHPEHVCGAYPYAYPPMPAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNSRLFHL
PEYTWRPPCAGIRNQSSQVMDPPPDRPAEPESADNDCDGPQ"
regulatory 1051..1142
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1143..1499
/note="3' FR of beta-globin"
regulatory complement(1577..1659)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1801..1876
/note="SV40 ori"
regulatory complement(1907..1981)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1907..1981
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2096..2497)
/note="pMB1 ori"
CDS complement(3038..3898)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87200.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4034..4411
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4417..5758
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4786..5710
/note="intron AG promoter"
BASE COUNT 1259 a 1560 c 1605 g 1337 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagagatgg aagagaccga caaggagcag ctgacagcag aggccaagga actgcgccag
121 aaggtcaggt acctacagga tcagctgagc ccgctcacaa ggcaacgaga ataccaggag
181 aaggagatcc agcggctcaa taaggccctg gaggaggccc tcagcatcca ggcctctcca
241 tcatctccgc ctgcagcttt tgggagtcca gaaggcgttg ggggccatct gaggaagcag
301 gaactagtga cacagaatga gttgctgaaa cagcaggtaa agatctttga agaggacttc
361 cagagggaac ggagtgaccg tgaacgcatg aatgaagaga aggaggagct gaagaagcaa
421 gtagagaagc tgcaggccca ggtcaccctg actaatgccc agctcaaaac tctcaaagag
481 gaggagaagg ccaaggaagc cctcaaacag cagaagagga aagcaaaggc ttcgggagag
541 cgctaccaca tggaacccca ccctgagcac gtctgcggcg cctatcccta tgcctaccca
601 cccatgccag ccatggtacc tcaccatgcc tacaaggact ggtcccagat ccgataccct
661 ccaccccctg tgcccatgga gcacccgccc ccacacccca actctcgcct cttccatctg
721 ccggagtaca cctggcgtcc accctgtgca gggattcgga atcagagctc tcaagtgatg
781 gacccgcccc cagacaggcc tgcagagcca gagtctgcag acaatgactg tgatgggccc
841 cagtgaggct gcagtgggtc atttggttcc accttcatct ttcagagcca gctgacctca
901 gattgccaaa agtttgaagg ccatgtgcat gttctgtgtg acccaagcct tggcagagga
961 gaggctggga tgggtagctg gctcacatcc ccagccaagc ctcgaactgt tgacaagacc
1021 cccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
1081 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
1141 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
1201 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
1261 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
1321 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
1381 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
1441 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
1501 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
1561 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggt
1621 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
1681 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
1741 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
1801 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
1861 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
1921 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
1981 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
2041 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
2101 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
2161 gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca ggaaagaaca
2221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
2281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
2341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
2401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
2461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
2521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
2581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
2641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
2701 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
2761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
2821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
2881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
2941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
3001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
3061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
3121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
3181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
3241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
3301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
3361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
3421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
3481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
3541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
3601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
3661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
3721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
3781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
3841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
3901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
3961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
4021 tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
4081 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
4141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
4201 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
4261 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
4321 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
4381 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
4441 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
4501 ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg
4561 ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg
4621 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
4681 gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc
4741 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
4801 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
4861 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
4921 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
4981 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
5041 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
5101 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
5161 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
5221 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
5281 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc
5341 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
5401 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
5461 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
5521 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
5581 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
5641 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
5701 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
5761 a
//