LOCUS LT727011 5449 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pcDNA3.1-Flag, complete sequence.
ACCESSION LT727011
VERSION LT727011.1
KEYWORDS .
SOURCE Mammalian expression vector pcDNA3.1-Flag
ORGANISM Mammalian expression vector pcDNA3.1-Flag
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5449)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5449
/organism="Mammalian expression vector pcDNA3.1-Flag"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5351."
/db_xref="taxon:1944925"
regulatory 235..818
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory 863..879
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 923..946
/note="FLAG epitope"
regulatory 1046..1270
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 1316..1744
/note="f1 ori"
regulatory 1749..2030
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 1987..2062
/note="SV40 ori"
CDS 2154..2948
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87195.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory complement(3122..3196)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 3122..3196
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3335..3418)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(3510..3912)
/note="pMB1 ori"
CDS complement(4453..5313)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87196.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1262 a 1411 c 1392 g 1384 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc
961 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
1021 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
1081 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
1141 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
1201 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
1261 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
1321 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
1381 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
1441 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
1501 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
1561 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
1621 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
1681 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
1741 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
1801 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
1861 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
1921 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
1981 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
2041 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
2101 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
2161 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
2221 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
2281 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
2341 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
2401 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
2461 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
2521 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
2581 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
2641 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
2701 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
2761 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
2821 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
2881 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
2941 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
3001 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
3061 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
3121 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
3181 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
3241 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
3301 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
3361 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
3421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
3481 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
3541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
3601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
3661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
3721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
3781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
3841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
3901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
3961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
4021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
4081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
4141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
4261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
4321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
4381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
4441 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
4501 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
4561 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
4621 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
4681 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
4741 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
4801 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
4861 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
4921 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
4981 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
5041 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
5101 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
5161 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
5221 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
5281 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
5341 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
5401 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//