LOCUS LT727010 5496 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCD-Lck-Flag, complete sequence. ACCESSION LT727010 VERSION LT727010.1 KEYWORDS . SOURCE Mammalian expression vector pCD-Lck-Flag ORGANISM Mammalian expression vector pCD-Lck-Flag other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5496) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5496 /organism="Mammalian expression vector pCD-Lck-Flag" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5350." /db_xref="taxon:1944909" regulatory 235..818 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 863..879 /note="T7 promoter" /regulatory_class="promoter" CDS 914..>952 /transl_table=11 /note="mLck myristoylation-targeting sequence" /protein_id="SJL87192.1" /translation="MGCVCSSNPEDDW" misc_feature 968..991 /note="FLAG epitope" regulatory 1091..1315 /note="BGH polyA" /regulatory_class="terminator" rep_origin 1361..1789 /note="f1 ori" regulatory 1794..2075 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 2032..2107 /note="SV40 ori" CDS 2199..2993 /transl_table=11 /note="Tn5 neomycin resistance" /protein_id="SJL87193.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 3169..3243 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(3169..3243) /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(3382..3465) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(3557..3959) /note="pMB1 ori" CDS complement(4500..5360) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87194.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1271 a 1423 c 1408 g 1394 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 1021 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga 1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 1261 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 1321 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 1381 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 1441 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 1501 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 1561 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 1621 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 1681 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 1741 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 1801 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 1861 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 1921 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 1981 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2041 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 2101 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 2161 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 2221 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 2281 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 2341 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 2401 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 2461 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 2521 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 2581 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 2641 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 2701 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 2761 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 2821 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 2881 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 2941 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 3001 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 3061 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 3121 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 3181 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 3241 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 3301 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 3361 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 3421 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 3481 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 3541 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 3601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 3661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 3721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 3781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 3841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 3901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 3961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4141 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 4201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 4321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 4381 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 4441 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 4501 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 4561 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 4621 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 4681 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 4741 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 4801 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 4861 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 4921 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 4981 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 5041 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 5101 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 5161 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 5221 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 5281 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 5341 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 5401 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 5461 ccgcgcacat ttccccgaaa agtgccacct gacgtc //