LOCUS LT727003 7393 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1hCASP-9-His, complete sequence.
ACCESSION LT727003
VERSION LT727003.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1hCASP-9-His
ORGANISM Mammalian expression vector pEF1hCASP-9-His
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7393)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7393
/organism="Mammalian expression vector pEF1hCASP-9-His"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5287."
/db_xref="taxon:1944976"
regulatory 508..1651
/note="hEF1 promoter"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron hEF1 promoter"
regulatory 1668..1684
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1758..>3005
/transl_table=11
/note="hCASP-9"
/protein_id="SJL87167.1"
/translation="MDEADRRLLRRCRLRLVEELQVDQLWDVLLSRELFRPHMIEDIQ
RAGSGSRRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASFLRTNRQAGKLSKPT
LENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESLRGNADLAYILSMEPC
GHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLE
LARQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLG
GKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISS
LPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVS
VKGIYKQMPGCFNFLRKKLFFKTS"
misc_feature 3024..3065
/note="V5 epitope"
misc_feature 3075..3092
/note="His-tag"
regulatory 3121..3345
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 3391..3819
/note="f1 ori"
regulatory 3824..4105
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4062..4137
/note="SV40 ori"
CDS 4229..5023
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87168.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5199..5273
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5199..5273)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(5412..5495)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5587..5978)
/note="pMB1 ori"
CDS complement(6519..7379)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87169.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1580 a 1939 c 2036 g 1838 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
1741 tgtggtggaa ttccgccatg gacgaagcgg atcggcggct cctgcggcgg tgccggctgc
1801 ggctggtgga agagctgcag gtggaccagc tctgggacgt cctgctgagc cgcgagctgt
1861 tcaggcccca tatgatcgag gacatccagc gggcaggctc tggatctcgg cgggatcagg
1921 ccaggcagct gatcatagat ctggagactc gagggagtca ggctcttcct ttgttcatct
1981 cctgcttaga ggacacaggc caggacatgc tggcttcgtt tctgcgaact aacaggcaag
2041 caggaaagtt gtcgaagcca accctagaaa accttacccc agtggtgctc agaccagaga
2101 ttcgcaaacc agaggttctc agaccggaaa cacccagacc agtggacatt ggttctggag
2161 gattcggtga tgtcggtgct cttgagagtt tgaggggaaa tgcagatttg gcttacatcc
2221 tgagcatgga gccctgtggc cactgcctca ttatcaacaa tgtgaacttc tgccgtgagt
2281 ccgggctccg cacccgcact ggctccaaca tcgactgtga gaagttgcgg cgtcgcttct
2341 cctcgctgca tttcatggtg gaggtgaagg gcgacctgac tgccaagaaa atggtgctgg
2401 ctttgctgga gctggcgcgg caggaccacg gtgctctgga ctgctgcgtg gtggtcattc
2461 tctctcacgg ctgtcaggcc agccacctgc agttcccagg ggctgtctac ggcacagatg
2521 gatgccctgt gtcggtcgag aagattgtga acatcttcaa tgggaccagc tgccccagcc
2581 tgggagggaa gcccaagctc tttttcatcc aggcctgtgg tggggagcag aaagaccatg
2641 ggtttgaggt ggcctccact tcccctgaag acgagtcccc tggcagtaac cccgagccag
2701 atgccacccc gttccaggaa ggtttgagga ccttcgacca gctggacgcc atatctagtt
2761 tgcccacacc cagtgacatc tttgtgtcct actctacttt cccaggtttt gtttcctgga
2821 gggaccccaa gagtggctcc tggtacgttg agaccctgga cgacatcttt gagcagtggg
2881 ctcactctga agacctgcag tccctcctgc ttagggtcgc taatgctgtt tcggtgaaag
2941 ggatttataa acagatgcct ggttgcttta atttcctccg gaaaaaactt ttctttaaaa
3001 catcatctag agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
3061 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
3121 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
3181 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
3241 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
3301 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
3361 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
3421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
3481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
3541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
3601 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
3661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
3721 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
3781 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
3841 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3901 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
3961 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
4021 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
4081 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
4141 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
4201 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
4261 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
4321 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
4381 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
4441 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
4501 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
4561 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
4621 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
4681 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
4741 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
4801 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
4861 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
4921 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
4981 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
5041 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
5101 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
5161 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
5341 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
5401 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
5461 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
5521 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
5581 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
5641 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
5701 caggaaagaa catgtgagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
5761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
5821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
5881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
5941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
6001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
6061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
6121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
6181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
6241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
6301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
6361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
6421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
6481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
6541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
6601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
6661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
6721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
6781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
6841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
6901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
6961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
7021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
7081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
7141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
7201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
7261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
7321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
7381 ctcttccttt ttc
//