LOCUS LT727001 3858 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pMET7hIL-2Ralpha, complete sequence.
ACCESSION LT727001
VERSION LT727001.1
KEYWORDS .
SOURCE Mammalian expression vector pMET7hIL-2Ralpha
ORGANISM Mammalian expression vector pMET7hIL-2Ralpha
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3858)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3858
/organism="Mammalian expression vector pMET7hIL-2Ralpha"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5280."
/db_xref="taxon:1945053"
regulatory 9..632
/note="SRa"
/regulatory_class="promoter"
rep_origin 247..322
/note="SV40 ori"
regulatory 800..816
/note="T7"
/regulatory_class="promoter"
misc_feature 861..923
/note="sIL2R"
CDS <924..1679
/codon_start=1
/transl_table=11
/note="hIL2R"
/protein_id="SJL87162.1"
/translation="ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYM
LCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPG
HCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQ
LICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEY
QVAVAGCVFLLISVLLLSGLTWQRRQRKSRRTI"
regulatory 1741..1837
/note="polyA"
/regulatory_class="terminator"
regulatory complement(1741..1837)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(1924..2325)
/note="pMB1 ori"
CDS complement(2866..3726)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87163.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 983 a 1016 c 917 g 942 t
ORIGIN
1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat
361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact
661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag
721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
781 tctaaaagct gcggaattct aatacgactc actataggga gtcgactcag atcttcgata
841 aacttaagct tggtaccacc atggattcat acctgctgat gtggggactg ctcacgttca
901 tcatggtgcc tggctgccag gcagagctct gtgacgatga cccgccagag atcccacacg
961 ccacattcaa agccatggcc tacaaggaag gaaccatgtt gaactgtgaa tgcaagagag
1021 gtttccgcag aataaaaagc gggtcactct atatgctctg tacaggaaac tctagccact
1081 cgtcctggga caaccaatgt caatgcacaa gctctgccac tcggaacaca acgaaacaag
1141 tgacacctca acctgaagaa cagaaagaaa ggaaaaccac agaaatgcaa agtccaatgc
1201 agccagtgga ccaagcgagc cttccaggtc actgcaggga acctccacca tgggaaaatg
1261 aagccacaga gagaatttat catttcgtgg tggggcagat ggtttattat cagtgcgtcc
1321 agggatacag ggctctacac agaggtcctg ctgagagcgt ctgcaaaatg acccacggga
1381 agacaaggtg gacccagccc cagctcatat gcacaggtga aatggagacc agtcagtttc
1441 caggtgaaga gaagcctcag gcaagccccg aaggccgtcc tgagagtgag acttcctgcc
1501 tcgtcacaac aacagatttt caaatacaga cagaaatggc tgcaaccatg gagacgtcca
1561 tatttacaac agagtaccag gtagcagtgg ccggctgtgt tttcctgctg atcagcgtcc
1621 tcctcctgag tgggctcacc tggcagcgga gacagaggaa gagtagaaga acaatctagg
1681 cggccgctcg agtctctaga gaaaaaacct cccacacctc cccctgaacc tgaaacataa
1741 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
1801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
1861 gtccaaactc atcaatgtat cttatcatgt ctggatcccc gggtaccgag ctcgaattaa
1921 ttcctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
2041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
2101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
2161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
2221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
2281 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
2341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
2401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
2461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
2521 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
2581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
2641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
3001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
3061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
3121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
3181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
3241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
3301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
3361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
3421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
3481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
3541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
3601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3721 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3841 ccgaaaagtg ccacctgc
//