LOCUS LT726998 5747 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-FLAG-hHuR, complete sequence. ACCESSION LT726998 VERSION LT726998.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-FLAG-hHuR ORGANISM Mammalian expression vector pCAGGS-FLAG-hHuR other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5747) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5747 /organism="Mammalian expression vector pCAGGS-FLAG-hHuR" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5270." /db_xref="taxon:1944830" misc_feature 13..36 /note="FLAG epitope" CDS 43..1023 /transl_table=11 /note="hHuR" /protein_id="SJL87155.1" /translation="MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIG EVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEV IKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEA EEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRF RFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVI RDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK" regulatory 1033..1124 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 1125..1481 /note="3' FR of beta-globin" regulatory complement(1559..1641) /note="lac promoter" /regulatory_class="promoter" rep_origin 1783..1858 /note="SV40 ori" regulatory complement(1889..1963) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 1889..1963 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(2078..2479) /note="pMB1 ori" CDS complement(3020..3880) /transl_table=11 /note="ampicillin" /protein_id="SJL87156.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4020..4397 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4403..5744 /note="AG promoter" /regulatory_class="promoter" misc_feature 4772..5696 /note="intron AG promoter" BASE COUNT 1256 a 1521 c 1592 g 1378 t ORIGIN 1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatgtctaa tggttatgaa 61 gaccacatgg ccgaagactg caggggtgac atcgggagaa cgaatttgat cgtcaactac 121 ctccctcaga acatgaccca ggatgagtta cgaagcctgt tcagcagcat tggtgaagtt 181 gaatctgcaa aacttattcg ggataaagta gcaggacaca gcttgggcta tggctttgtg 241 aactacgtga ccgcgaagga tgcagagaga gcgatcaaca cgctgaacgg cttgaggctc 301 cagtcaaaaa ccattaaggt gtcgtatgct cgcccgagct cagaggtgat caaagacgcc 361 aacttgtaca tcagcgggct cccgcggacc atgacccaga aggacgtaga agacatgttc 421 tctcggtttg ggcggatcat caactcgcgg gtcctcgtgg atcagactac aggtttgtcc 481 agaggggttg cgtttatccg gtttgacaaa cggtcggagg cagaagaggc aattaccagt 541 ttcaatggtc ataaaccccc aggttcctct gagcccatca cagtgaagtt tgcagccaac 601 cccaaccaga acaaaaacgt ggcactcctc tcgcagctgt accactcgcc agcgcgacgg 661 ttcggaggcc ccgttcacca ccaggcgcag agattcaggt tctcccccat gggcgtcgat 721 cacatgagcg ggctctctgg cgtcaacgtg ccaggaaacg cctcctccgg ctggtgcatt 781 ttcatctaca acctggggca ggatgccgac gaggggatcc tctggcagat gtttgggccg 841 tttggtgccg tcaccaatgt gaaagtgatc cgcgacttca acaccaacaa gtgcaaaggg 901 tttggctttg tgaccatgac aaactatgaa gaagccgcga tggccatagc cagcctgaac 961 ggctaccgcc tgggggacaa aatcttacag gtttccttca aaaccaacaa gtcccacaaa 1021 taagtcgacg aagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 1081 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 1141 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 1201 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 1261 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 1321 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 1381 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 1441 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag 1501 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 1561 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa 1621 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 1681 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 1741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 1801 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 1861 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa 1921 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 1981 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc 2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 2161 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc 2221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 2461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 2881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 2941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 3661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 3901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 3961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg 4021 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 4081 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 4141 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 4201 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 4261 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 4321 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 4381 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 4441 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 4501 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg 4561 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 4621 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 4681 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 4741 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 4801 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 4861 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 4921 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 4981 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 5041 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 5101 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 5161 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 5221 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 5281 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg 5341 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 5401 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 5461 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 5521 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 5581 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 5641 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 5701 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa //