LOCUS LT726997 6161 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAG-hHNRPK, complete sequence.
ACCESSION LT726997
VERSION LT726997.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAG-hHNRPK
ORGANISM Mammalian expression vector pCAGGS-FLAG-hHNRPK
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6161)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6161
/organism="Mammalian expression vector pCAGGS-FLAG-hHNRPK"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5269."
/db_xref="taxon:1944829"
misc_feature 13..36
/note="FLAG epitope"
CDS 43..1437
/transl_table=11
/note="hHNRPK"
/protein_id="SJL87153.1"
/translation="METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVE
LRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILK
KIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGII
GVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPI
KGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPS
RRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYD
GMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQV
TIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYL
LQNSVKQYADVEGF"
regulatory 1447..1538
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1539..1895
/note="3' FR of beta-globin"
regulatory complement(1973..2055)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2197..2272
/note="SV40 ori"
regulatory complement(2303..2377)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 2303..2377
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2492..2893)
/note="pMB1 ori"
CDS complement(3434..4294)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87154.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4434..4811
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4817..6158
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5186..6110
/note="intron AG promoter"
BASE COUNT 1403 a 1561 c 1684 g 1513 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggaaac tgaacagcca
61 gaagaaacct tccctaacac tgaaaccaat ggtgaatttg gtaaacgccc tgcagaagat
121 atggaagagg aacaagcatt taaaagatct agaaacactg atgagatggt tgaattacgc
181 attctgcttc agagcaagaa tgctggggca gtgattggaa aaggaggcaa gaatattaag
241 gctctccgta cagactacaa tgccagtgtt tcagtcccag acagcagtgg ccccgagcgc
301 atattgagta tcagtgctga tattgaaaca attggagaaa ttctgaagaa aatcatccct
361 accttggaag agggcctgca gttgccatca cccactgcaa ccagccagct cccgctcgaa
421 tctgatgctg tggaatgctt aaattaccaa cactataaag gaagtgactt tgactgcgag
481 ttgaggctgt tgattcatca gagtctagca ggaggaatta ttggggtcaa aggtgctaaa
541 attaaagaac ttcgagagaa cactcaaacc accatcaagc ttttccagga atgctgtcct
601 cattccactg acagagttgt tcttattgga ggaaaacccg atagggttgt agagtgcata
661 aagatcatcc ttgatcttat atctgagtct cccatcaaag gacgtgcaca gccttatgat
721 cccaattttt acgatgaaac ctatgattat ggtggtttta caatgatgtt tgatgaccgt
781 cgcggacgcc cagtgggatt tcccatgcgg ggaagaggtg gttttgacag aatgcctcct
841 ggtcggggtg ggcgtcccat gcctccatct agaagagatt atgatgatat gagccctcgt
901 cgaggaccac ctccccctcc tcccggacga ggcggccggg gtggtagcag agctcggaat
961 cttcctcttc ctccaccacc accacctaga gggggagacc tcatggccta tgacagaaga
1021 gggagacctg gagaccgtta cgacggcatg gttggtttca gtgctgatga aacttgggac
1081 tctgcaatag atacatggag cccatcagaa tggcagatgg cttatgaacc acagggtggc
1141 tccggatatg attattccta tgcagggggt cgtggctcat atggtgatct tggtggacct
1201 attattacta cacaagtaac tattcccaaa gatttggctg gatctattat tggcaaaggt
1261 ggtcagcgga ttaaacaaat ccgtcatgag tcgggagctt cgatcaaaat tgatgagcct
1321 ttagaaggat ccgaagatcg gatcattacc attacaggaa cacaggacca gatacagaat
1381 gcacagtatt tgctgcagaa cagtgtgaag cagtatgcag atgttgaagg attctaagtc
1441 gacgaagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
1501 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
1561 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
1621 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
1681 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
1741 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
1801 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
1861 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
1921 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
1981 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca
2041 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
2101 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
2161 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
2221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
2281 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
2341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
2401 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
2461 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
2521 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
2581 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
2641 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2701 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2761 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2821 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2881 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2941 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
3001 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
3061 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
3121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
3181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3241 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3301 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3361 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3421 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
3481 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
3541 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
3601 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
3661 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
3721 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
3781 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
3841 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
3901 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
3961 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
4021 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
4081 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
4141 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
4201 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
4261 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
4321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg atcgacattg
4441 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
4501 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
4561 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
4621 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
4681 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
4741 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
4801 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
4861 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
4921 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
4981 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
5041 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt
5101 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
5161 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
5221 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
5281 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
5341 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
5401 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
5461 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
5521 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
5581 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
5641 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
5701 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc
5761 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
5821 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
5881 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
5941 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
6001 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
6061 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
6121 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a
//