LOCUS LT726996 5819 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAG-hUNRIP, complete sequence.
ACCESSION LT726996
VERSION LT726996.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAG-hUNRIP
ORGANISM Mammalian expression vector pCAGGS-FLAG-hUNRIP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5819)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5819
/organism="Mammalian expression vector pCAGGS-FLAG-hUNRIP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5268."
/db_xref="taxon:1944842"
misc_feature 13..36
/note="FLAG epitope"
CDS 43..1095
/transl_table=11
/note="hUNRIP"
/protein_id="SJL87151.1"
/translation="MAMRQTPLTCSGHTRPVVDLAFSGITPYGYFLISACKDGKPMLR
QGDTGDWIGTFLGHKGAVWGATLNKDATKAATAAADFTAKVWDAVSGDELMTLAHKHI
VKTVDFTQDSNYLLTGGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQILS
ADDKTVRLWDHATMTEVKSLNFNMSVSSMEYIPEGEILVITYGRSIAFHSAVSLDPIK
SFEAPATINSASLHPEKEFLVAGGEDFKLYKYDYNSGEELESYKGHFGPIHCVRFSPD
GELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEEDSGELAKPKIGFPETTEEELEEIA
SENSECIFPSAPDVKA"
regulatory 1105..1196
/note="beta-globin polyA"
/regulatory_class="terminator"
regulatory complement(1631..1713)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1855..1930
/note="SV40 ori"
regulatory complement(1961..2035)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1961..2035
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2150..2551)
/note="pMB1 ori"
CDS complement(3092..3952)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87152.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4092..4469
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4475..5816
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4844..5768
/note="intron AG promoter"
BASE COUNT 1313 a 1448 c 1585 g 1473 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggcaat gagacagacg
61 ccgctcacct gctctggcca cacgcgaccc gtggttgatt tggccttcag tggcatcacg
121 ccttatgggt atttcttaat cagcgcttgc aaagatggta aacctatgct acgccaggga
181 gatacaggag actggattgg aacatttttg ggtcataaag gtgctgtttg gggtgcaaca
241 ctgaataagg atgccaccaa agcagctaca gcagctgcag atttcacagc caaagtgtgg
301 gatgctgtct caggagatga attgatgacc ctggctcata aacacattgt caagactgtg
361 gatttcacgc aggatagtaa ttatttgtta accgggggac aggataaact gttacgcata
421 tatgacttga acaaacctga agcagaacct aaggaaatta gtggtcatac ttctggtata
481 aaaaaagctc tgtggtgcag tgaggataaa cagattcttt ctgctgatga caaaactgtt
541 cgactttggg atcatgctac tatgacagaa gtgaaatctc taaattttaa tatgtctgtt
601 agtagtatgg aatatattcc tgagggagag attttggtta taacttatgg acgatctatt
661 gcttttcata gtgcagtaag tttggaccca attaaatcct ttgaagctcc tgcaaccatc
721 aattctgcat ctcttcatcc tgagaaagaa tttcttgttg caggcggtga agattttaaa
781 ctttataagt atgattataa tagtggagaa gaattagaat cctacaaggg acactttggt
841 cctattcact gtgtgagatt tagtcctgat ggagaactct atgccagtgg ttcagaagat
901 ggaacattga gactatggca aactgtggta ggaaaaacgt atggcctttg gaaatgtgtg
961 cttcctgaag aagatagtgg tgagctggca aagccaaaga ttggttttcc agagacaaca
1021 gaagaggagc tagaagaaat tgcttcagag aattcagagt gcatctttcc ttcagctcct
1081 gatgttaagg cctgagtcga cgaagatctt tttccctctg ccaaaaatta tggggacatc
1141 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
1201 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
1261 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
1321 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
1381 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
1441 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
1501 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
1561 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
1621 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa
1681 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
1741 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
1921 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
1981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
2041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
2101 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
2161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
2221 tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag
2281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
2341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
2521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
2941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
3001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
3061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
3121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
3181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
3241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
3301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
3361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
3421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
3481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
3541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
3601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
3661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
3721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
3781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
3841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
3901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
3961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
4021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
4081 ctgggtcgat cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
4141 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
4201 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
4261 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
4321 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
4381 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
4441 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
4501 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
4561 ttgtgcagcg atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg
4621 cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct
4681 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
4741 gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc
4801 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
4861 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
4921 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
4981 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
5041 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
5101 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
5161 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
5221 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
5281 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
5341 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc
5401 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
5461 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
5521 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
5581 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
5641 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
5701 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
5761 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa
//