LOCUS LT726992 5246 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV-Sport-Raf-1E, complete sequence.
ACCESSION LT726992
VERSION LT726992.1
KEYWORDS .
SOURCE Mammalian expression vector pSV-Sport-Raf-1E
ORGANISM Mammalian expression vector pSV-Sport-Raf-1E
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5246)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5246
/organism="Mammalian expression vector pSV-Sport-Raf-1E"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5264."
/db_xref="taxon:1945160"
regulatory 1..282
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 239..314
/note="SV40 ori"
regulatory 345..361
/note="SP6 promoter"
/regulatory_class="promoter"
CDS 395..>2338
/transl_table=11
/note="hRaf1"
/protein_id="SJL87143.1"
/translation="MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASD
DGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLL
HEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLL
NGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMR
ESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRM
IEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRG
QRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRN
EVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIAR
QTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGS
VLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYA
SPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPS
LHRAAHTEDINACTLTTSPRLPVF"
misc_feature 2348..2386
/note="E-tag"
regulatory complement(2566..2582)
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 2671..2710
/note="intron of SV40 small t-antigen"
regulatory complement(2947..3043)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 2947..3043
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(3156..3558)
/note="pMB1 ori"
CDS complement(4089..4949)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87144.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5145..5202
/note="lacUV5 promoter"
/regulatory_class="promoter"
BASE COUNT 1391 a 1291 c 1218 g 1346 t
ORIGIN
1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta
241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat
361 agaaggtacg cctgcaggta ccggtccgga attcatggag cacatacagg gagcttggaa
421 gacgatcagc aatggttttg gattcaaaga tgccgtgttt gatggctcca gctgcatctc
481 tcctacaata gttcagcagt ttggctatca gcgccgggca tcagatgatg gcaaactcac
541 agatccttct aagacaagca acactatccg tgttttcttg ccgaacaagc aaagaacagt
601 ggtcaatgtg cgaaatggaa tgagcttgca tgactgcctt atgaaagcac tcaaggtgag
661 gggcctgcaa ccagagtgct gtgcagtgtt cagacttctc cacgaacaca aaggtaaaaa
721 agcacgctta gattggaata ctgatgctgc gtctttgatt ggagaagaac ttcaagtaga
781 tttcctggat catgttcccc tcacaacaca caactttgct cggaagacgt tcctgaagct
841 tgccttctgt gacatctgtc agaaattcct gctcaatgga tttcgatgtc agacttgtgg
901 ctacaaattt catgagcact gtagcaccaa agtacctact atgtgtgtgg actggagtaa
961 catcagacaa ctcttattgt ttccaaattc cactattggt gatagtggag tcccagcact
1021 accttctttg actatgcgtc gtatgcgaga gtctgtttcc aggatgcctg ttagttctca
1081 gcacagatat tctacacctc acgccttcac ctttaacacc tccagtccct catctgaagg
1141 ttccctctcc cagaggcaga ggtcgacatc cacacctaat gtccacatgg tcagcaccac
1201 cctgcctgtg gacagcagga tgattgagga tgcaattcga agtcacagcg aatcagcctc
1261 accttcagcc ctgtccagta gccccaacaa tctgagccca acaggctggt cacagccgaa
1321 aacccccgtg ccagcacaaa gagagcgggc accagtatct gggacccagg agaaaaacaa
1381 aattaggcct cgtggacaga gagattcaag ctattattgg gaaatagaag ccagtgaagt
1441 gatgctgtcc actcggattg ggtcaggctc ttttggaact gtttataagg gtaaatggca
1501 cggagatgtt gcagtaaaga tcctaaaggt tgtcgaccca accccagagc aattccaggc
1561 cttcaggaat gaggtggctg ttctgcgcaa aacacggcat gtgaacattc tgcttttcat
1621 ggggtacatg acaaaggaca acctggcaat tgtgacccag tggtgcgagg gcagcagcct
1681 ctacaaacac ctgcatgtcc aggagaccaa gtttcagatg ttccagctaa ttgacattgc
1741 ccggcagacg gctcagggaa tggactattt gcatgcaaag aacatcatcc atagagacat
1801 gaaatccaac aatatatttc tccatgaagg cttaacagtg aaaattggag attttggttt
1861 ggcaacagta aagtcacgct ggagtggttc tcagcaggtt gaacaaccta ctggctctgt
1921 cctctggatg gccccagagg tgatccgaat gcaggataac aacccattca gtttccagtc
1981 ggatgtctac tcctatggca tcgtattgta tgaactgatg acgggggagc ttccttattc
2041 tcacatcaac aaccgagatc agatcatctt catggtgggc cgaggatatg cctccccaga
2101 tcttagtaag ctatataaga actgccccaa agcaatgaag aggctggtag ctgactgtgt
2161 gaagaaagta aaggaagaga ggcctctttt tccccagatc ctgtcttcca ttgagctgct
2221 ccaacactct ctaccgaaga tcaaccggag cgcttccgag ccatccttgc atcgggcagc
2281 ccacactgag gatatcaatg cttgcacgct gaccacgtcc ccgaggctgc ctgtcttcgc
2341 ggccgcaggt gcgccggtgc cgtatccgga tccgctggaa ccgcgtgccg catagactgt
2401 tgaaagttgt ttagcaaaac ctcatacaga aaattcattt actaacgtct ggaaagacga
2461 caaaacttta gatcgttacg ctaactatga gggctgtctg tggaatgtcg actctagagg
2521 atccaagctt acgtacgcgt gcatgcgacg tcatagctct ctccctatag tgagtcgtat
2581 tataagctag cttgggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
2641 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
2701 tgttaaacta gctgcatatg cttgctgctt gagagttttg cttactgagt atgatttatg
2761 aaaatattat acacaggagc tagtgattct aattgtttgt gtattttaga ttcacagtcc
2821 caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa tcagccatac
2881 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
2941 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
3001 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
3061 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcctgcatt aatgaatcgg
3121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
3181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
3241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
3301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
3361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
3421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
3481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
3541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
3601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
3661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
3721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
3781 gacagtattt ggtatctgcg gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
3841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
3901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
3961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
4021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
4081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
4141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
4201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
4261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
4321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
4381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
4441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
4501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
4561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
4621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
4681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
4741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
4801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
4861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
4921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
4981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
5041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
5101 atcatgacat taacctataa aaataggcgt atacgaggcc ctttcactca ttaggcaccc
5161 caggctttac actttatagc ttccggctcg tataatgtgt ggaattgtga gcggataaca
5221 atttcacaca ggaaacagca tcgatg
//