LOCUS LT726991 8987 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV-Sport-Fluc-PITSLRE-LacZ, complete
sequence.
ACCESSION LT726991
VERSION LT726991.1
KEYWORDS .
SOURCE Mammalian expression vector pSV-Sport-Fluc-PITSLRE-LacZ
ORGANISM Mammalian expression vector pSV-Sport-Fluc-PITSLRE-LacZ
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8987)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8987
/organism="Mammalian expression vector
pSV-Sport-Fluc-PITSLRE-LacZ"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5262."
/db_xref="taxon:1945157"
regulatory 1..282
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 239..314
/note="SV40 ori"
regulatory 345..361
/note="SP6 promoter"
/regulatory_class="promoter"
CDS 465..2117
/transl_table=11
/note="LUCm"
/protein_id="SJL87140.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
misc_feature 2125..3082
/note="Di-1 fragment of p110 PITSLRE (IRES)"
CDS 3085..6144
/transl_table=11
/note="lacZ"
/protein_id="SJL87141.1"
/translation="MEDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS
QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP
ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD
SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE
NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHP
LWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVI
IWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPF
PAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDW
VDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQ
FRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELP
QPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSE
MDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRID
PNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDG
SGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRW
DLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLL
HAEEGTWLNIDGFHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
regulatory complement(6307..6323)
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 6412..6451
/note="intron SV40 small t-antigen"
regulatory 6688..6784
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(6688..6784)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(6897..7299)
/note="pMB1 ori"
CDS complement(7830..8690)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87142.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 8886..8943
/note="lacUV5 promoter"
/regulatory_class="promoter"
BASE COUNT 2333 a 2184 c 2394 g 2076 t
ORIGIN
1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta
241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat
361 agaaggtacg cctgcaggta ccggagctct tacgcgtgct agcccgggct cgagatctgc
421 gatctaagta agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
481 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
601 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
781 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
841 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
901 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
1021 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
1081 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
1141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
1201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
1261 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
1321 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
1381 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
1441 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
1501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
1561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
1621 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
1681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
1741 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
1801 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
1861 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
1921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
1981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
2041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
2101 gaaagatcgc cgtgtaattc tagaaaagtg aaaactttag atgaaattct tcaggaaaag
2161 aaacgaagga aggaacaaga ggagaaagca gagataaaac gcttaaaaaa ttctgatgac
2221 cgggattcca agcgggattc ccttgaggag ggggagctga gagatcactg catggagatc
2281 acaataagga actccccgta tagaagagaa gactcaatgg aagacagagg agaagaagat
2341 gattctttgg ccatcaaacc accccagcaa atgtcttgga aagaaaaagt tcatcacaga
2401 aaagatgaaa agaggaaaga aaaatggaag catgctagag tgaaagaaag agagcacgaa
2461 cgtcggaaac gacatcgaga agaacaggat aaagctcgcc gggaatggga aagacagaag
2521 agaagggaaa tggcaaggga gcattccagg agagaaaggg accgcttgga gcagttagaa
2581 aggaagcggg agcgggagcg caagatgcgg gagcagcaga aggagcagcg ggagcagaag
2641 gagcgcgagc ggcgggcgga ggagcggcgc aaggagcggg aggcccgcag ggaagtgtct
2701 gcacatcacc gaacgatgag agaggactac agcgacaaag tgaaagccag ccactggagt
2761 cgcagcccgc ctcggccgcc gcgggagcgg ttcgagttgg gagacggccg gaagccagta
2821 aaagaagaga aaatggaaga aagggacctg ctgtccgact tacaggacat cagcgacagc
2881 gagaggaaga ccagctcggc cgagtcctcg tcagcagaat caggctcagg ttctgaggaa
2941 gaagaggagg aggaggaaga ggaggaggag gaagggagca ccagtgaaga accagaggag
3001 gaagaggaag aggaggagga ggagaccggc agcaactctg aggaggcatc agagcagtct
3061 gccgaagaag taagtgagga agccatggaa gatcccgtcg ttttacaacg tcgtgactgg
3121 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
3181 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
3241 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
3301 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
3361 gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg
3421 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
3481 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg
3541 cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
3601 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat
3661 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
3721 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
3781 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
3841 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
3901 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa
3961 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
4021 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
4081 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
4141 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
4201 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
4261 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
4321 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
4381 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
4441 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
4501 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
4561 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
4621 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
4681 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
4741 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
4801 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
4861 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
4921 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
4981 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
5041 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
5101 aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc
5161 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
5221 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc
5281 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
5341 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg
5401 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
5461 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
5521 caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt
5581 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
5641 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca
5701 ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac
5761 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac
5821 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
5881 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
5941 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
6001 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
6061 gactcctgga gcccgtcagt atcggcggaa ttacagctga gcgccggtcg ctaccattac
6121 cagttggtct ggtgtcaaaa ataataataa ccggcaggcc atgtctgccc gtattcgcgt
6181 aagaaatcca ttatgtacta tttgggcgag ctcgaattgg ctagagtcga cgagctcact
6241 agtcggcggc cgctctagag gatccaagct tacgtacgcg tgcatgcgac gtcatagctc
6301 tctccctata gtgagtcgta ttataagcta gcttgggatc tttgtgaagg aaccttactt
6361 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
6421 aaaattttta agtgtataat gtgttaaact agctgcatat gcttgctgct tgagagtttt
6481 gcttactgag tatgatttat gaaaatatta tacacaggag ctagtgattc taattgtttg
6541 tgtattttag attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt
6601 catgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
6661 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
6721 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
6781 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
6841 gatcctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
6901 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
6961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
7021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
7081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
7141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
7201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
7261 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
7321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
7381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
7441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
7501 taactacggc tacactagaa ggacagtatt tggtatctgc ggccagttac cttcggaaaa
7561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
7621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
7681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
7741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
7801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
7861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
7921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
7981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
8041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
8101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
8161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
8221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
8281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
8341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
8401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
8461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
8521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
8581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
8641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
8701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
8761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
8821 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatacgaggc
8881 cctttcactc attaggcacc ccaggcttta cactttatag cttccggctc gtataatgtg
8941 tggaattgtg agcggataac aatttcacac aggaaacagc atcgatg
//