LOCUS LT726990 6535 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hPTB, complete sequence.
ACCESSION LT726990
VERSION LT726990.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hPTB
ORGANISM Mammalian expression vector pCAGGS-E-hPTB
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6535)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6535
/organism="Mammalian expression vector pCAGGS-E-hPTB"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5260."
/db_xref="taxon:1944778"
misc_feature 11..49
/note="E-tag"
CDS 59..1732
/transl_table=11
/note="hPTB"
/protein_id="SJL87138.1"
/translation="MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDS
KKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMN
TEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSG
NLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTK
NNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYT
RPDLPSGDSQPSLDQTMAAAFGAPGIISASPYAGAGFPPTFAIPQAAGLSVPNVHGAL
APLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQR
VKILFNKKENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQ
GLTKDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLFSSNGGVVK
GFKFFQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKSTI"
regulatory 1820..1911
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1912..2268
/note="3' FR of beta-globin"
regulatory complement(2346..2428)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2570..2645
/note="SV40 ori"
regulatory 2676..2750
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2676..2750)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2865..3266)
/note="pMB1 ori"
CDS complement(3807..4667)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87139.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4803..5180
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5186..6527
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5555..6479
/note="intron AG promoter"
BASE COUNT 1377 a 1848 c 1815 g 1495 t
ORIGIN
1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat
61 ggacggcatt gtcccagata tagccgttgg tacaaagcgg ggatctgacg agcttttctc
121 tacttgtgtc actaacggac cgtttatcat gagcagcaac tcggcttctg cagcaaacgg
181 aaatgacagc aagaagttca aaggtgacag ccgaagtgca ggcgtcccct ctagagtgat
241 ccacatccgg aagctcccca tcgacgtcac ggagggggaa gtcatctccc tggggctgcc
301 ctttgggaag gtcaccaacc tcctgatgct gaaggggaaa aaccaggcct tcatcgagat
361 gaacacggag gaggctgcca acaccatggt gaactactac acctcggtga cccctgtgct
421 gcgcggccag cccatctaca tccagttctc caaccacaag gagctgaaga ccgacagctc
481 tcccaaccag gcgcgggccc aggcggccct gcaggcggtg aactcggtcc agtcggggaa
541 cctggccttg gctgcctcgg cggcggccgt ggacgcaggg atggcgatgg ccgggcagag
601 ccccgtgctc aggatcatcg tggagaacct cttctaccct gtgaccctgg atgtgctgca
661 ccagattttc tccaagttcg gcacagtgtt gaagatcatc accttcacca agaacaacca
721 gttccaggcc ctgctgcagt atgcggaccc cgtgagcgcc cagcacgcca agctgtcgct
781 ggacgggcag aacatctaca acgcctgctg cacgctgcgc atcgactttt ccaagctcac
841 cagcctcaac gtcaagtaca acaatgacaa gagccgtgac tacacacgcc cagacctgcc
901 ttccggggac agccagccct cgctggacca gaccatggcc gcggccttcg gtgcacctgg
961 tataatctca gcctctccgt atgcaggagc tggtttccct cccacctttg ccattcctca
1021 agctgcaggc ctttccgttc cgaacgtcca cggcgccctg gcccccctgg ccatcccctc
1081 ggcggcggcg gcagctgcgg cggcaggtcg gatcgccatc ccgggcctgg cgggggcagg
1141 aaattctgta ttgctggtca gcaacctcaa cccagagaga gtcacacccc aaagcctctt
1201 tattcttttc ggcgtctacg gtgacgtgca gcgcgtgaag atcctgttca ataagaagga
1261 gaacgcccta gtgcagatgg cggacggcaa ccaggcccag ctggccatga gccacctgaa
1321 cgggcacaag ctgcacggga agcccatccg catcacgctc tcgaagcacc agaacgtgca
1381 gctgccccgc gagggccagg aggaccaggg cctgaccaag gactacggca actcacccct
1441 gcaccgcttc aagaagccgg gctccaagaa cttccagaac atattcccgc cctcggccac
1501 gctgcacctc tccaacatcc cgccctcagt ctccgaggag gatctcaagg tcctgttttc
1561 cagcaatggg ggcgtcgtca aaggattcaa gttcttccag aaggaccgca agatggcact
1621 gatccagatg ggctccgtgg aggaggcggt ccaggccctc attgacctgc acaaccacga
1681 cctcggggag aaccaccacc tgcgggtctc cttctccaag tccaccatct agctcgagga
1741 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
1801 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
1861 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
1921 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
1981 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
2041 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
2101 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
2161 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
2221 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
2281 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
2341 caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt
2401 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
2461 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
2521 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
2581 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
2641 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
2701 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
2761 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
2821 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
2881 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
2941 ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa
3001 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
3061 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
3121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
3181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
3241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
3301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
3361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
3421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
3481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
3541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
3601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
3661 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
3721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
3781 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
3841 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
3901 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
3961 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
4021 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
4081 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
4141 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
4201 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
4261 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
4321 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
4381 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
4441 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
4501 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
4561 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
4621 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
4681 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
4741 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
4801 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
4861 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
4921 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
4981 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
5041 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
5101 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
5161 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
5221 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
5281 gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg
5341 gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
5401 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
5461 gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc
5521 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
5581 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
5641 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
5701 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
5761 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
5821 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
5881 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
5941 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
6001 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
6061 ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg
6121 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
6181 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
6241 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
6301 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
6361 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
6421 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
6481 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgaa
//