LOCUS LT726989 6373 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hPTBdelnls, complete sequence.
ACCESSION LT726989
VERSION LT726989.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hPTBdelnls
ORGANISM Mammalian expression vector pCAGGS-E-hPTBdelnls
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6373)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6373
/organism="Mammalian expression vector
pCAGGS-E-hPTBdelnls"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5259."
/db_xref="taxon:1944779"
misc_feature 11..49
/note="E-tag"
CDS 59..1570
/transl_table=11
/note="hPTBdelnls"
/protein_id="SJL87136.1"
/translation="MVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAF
IEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNS
VQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKII
TFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKS
RDYTRPDLPSGDSQPSLDQTMAAAFGAPGIISASPYAGAGFPPTFAIPQAAGLSVPNV
HGALAPLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYG
DVQRVKILFNKKENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREG
QEDQGLTKDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLFSSNG
GVVKGFKFFQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKSTI"
regulatory 1658..1749
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1750..2106
/note="3' FR of beta-globin"
regulatory complement(2184..2266)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2408..2483
/note="SV40 ori"
regulatory 2514..2588
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2514..2588)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2703..3104)
/note="pMB1 ori"
CDS complement(3645..4505)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87137.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4641..5018
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5024..6365
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5393..6317
/note="intron AG promoter"
BASE COUNT 1332 a 1810 c 1771 g 1460 t
ORIGIN
1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat
61 ggtcccctct agagtgatcc acatccggaa gctccccatc gacgtcacgg agggggaagt
121 catctccctg gggctgccct ttgggaaggt caccaacctc ctgatgctga aggggaaaaa
181 ccaggccttc atcgagatga acacggagga ggctgccaac accatggtga actactacac
241 ctcggtgacc cctgtgctgc gcggccagcc catctacatc cagttctcca accacaagga
301 gctgaagacc gacagctctc ccaaccaggc gcgggcccag gcggccctgc aggcggtgaa
361 ctcggtccag tcggggaacc tggccttggc tgcctcggcg gcggccgtgg acgcagggat
421 ggcgatggcc gggcagagcc ccgtgctcag gatcatcgtg gagaacctct tctaccctgt
481 gaccctggat gtgctgcacc agattttctc caagttcggc acagtgttga agatcatcac
541 cttcaccaag aacaaccagt tccaggccct gctgcagtat gcggaccccg tgagcgccca
601 gcacgccaag ctgtcgctgg acgggcagaa catctacaac gcctgctgca cgctgcgcat
661 cgacttttcc aagctcacca gcctcaacgt caagtacaac aatgacaaga gccgtgacta
721 cacacgccca gacctgcctt ccggggacag ccagccctcg ctggaccaga ccatggccgc
781 ggccttcggt gcacctggta taatctcagc ctctccgtat gcaggagctg gtttccctcc
841 cacctttgcc attcctcaag ctgcaggcct ttccgttccg aacgtccacg gcgccctggc
901 ccccctggcc atcccctcgg cggcggcggc agctgcggcg gcaggtcgga tcgccatccc
961 gggcctggcg ggggcaggaa attctgtatt gctggtcagc aacctcaacc cagagagagt
1021 cacaccccaa agcctcttta ttcttttcgg cgtctacggt gacgtgcagc gcgtgaagat
1081 cctgttcaat aagaaggaga acgccctagt gcagatggcg gacggcaacc aggcccagct
1141 ggccatgagc cacctgaacg ggcacaagct gcacgggaag cccatccgca tcacgctctc
1201 gaagcaccag aacgtgcagc tgccccgcga gggccaggag gaccagggcc tgaccaagga
1261 ctacggcaac tcacccctgc accgcttcaa gaagccgggc tccaagaact tccagaacat
1321 attcccgccc tcggccacgc tgcacctctc caacatcccg ccctcagtct ccgaggagga
1381 tctcaaggtc ctgttttcca gcaatggggg cgtcgtcaaa ggattcaagt tcttccagaa
1441 ggaccgcaag atggcactga tccagatggg ctccgtggag gaggcggtcc aggccctcat
1501 tgacctgcac aaccacgacc tcggggagaa ccaccacctg cgggtctcct tctccaagtc
1561 caccatctag ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
1621 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
1681 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
1741 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
1801 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
1861 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
1921 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
1981 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
2041 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
2101 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
2161 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
2221 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
2281 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
2341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
2401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
2461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
2521 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
2581 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
2641 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
2701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
2761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
2821 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
2881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
2941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
3241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
3301 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
3361 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
3421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
3481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
3541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
3601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
3661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
3721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
3781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
3841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
3901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
3961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
4381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
4441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
4501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
4561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
4621 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
4681 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
4741 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
4801 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
4861 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
4921 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
4981 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
5041 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
5101 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
5161 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
5221 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
5281 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
5341 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
5401 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
5461 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
5521 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
5581 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
5641 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
5701 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
5761 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
5821 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
5881 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
5941 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
6001 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
6061 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
6121 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
6181 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
6241 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
6301 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
6361 gcaaagaatt gaa
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