LOCUS LT726981 6986 bp DNA circular SYN 06-FEB-2017
DEFINITION Suicide vector pKNG101, complete sequence.
ACCESSION LT726981
VERSION LT726981.1
KEYWORDS .
SOURCE Suicide vector pKNG101
ORGANISM Suicide vector pKNG101
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6986)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6986
/organism="Suicide vector pKNG101"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5246."
/db_xref="taxon:1945229"
CDS 696..1499
/transl_table=11
/note="StrA"
/note="streptomycin resistance"
/protein_id="SJL87110.1"
/translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLL
ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
CDS 1499..2335
/transl_table=11
/note="StrB"
/note="streptomycin resistance"
/protein_id="SJL87111.1"
/translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
SY"
rep_origin 2373..2760
/note="R6K ori"
rep_origin complement(3477..3587)
/note="oriT"
CDS 4001..4504
/transl_table=11
/note="insB"
/protein_id="SJL87112.1"
/translation="MPGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
EQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQ"
CDS complement(4642..6063)
/transl_table=11
/note="sacB"
/protein_id="SJL87113.1"
/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS
HITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG
YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT
QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF
DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES
LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS
NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN
KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN
IKGKKTSVVKDSILEQGQLTVNK"
BASE COUNT 1641 a 1677 c 1765 g 1903 t
ORIGIN
1 gtcgacggat ccccgggtac cataagtaga agcagcaacc caagtagctt taccagcatc
61 cggttcaacc agcatagtaa gaatcttact ggacatcggc agttcttcga acagtgcgcc
121 aactaccagc tctttctgca gttcattcag ggcaccggag aacctgcgtg caatccatct
181 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat ccatggatta cgcgttaacc
241 cgggcccgcg gatgcatatg atcttaaggc ctaggtctag agtctttgtt ttgacgccat
301 tagcgtacgt aacaatcctc gttaaaggac aaggacctga gcggaagtgt atcgtacagt
361 agacggagta tactagtata gtctatagtc cgtggaatta ttatatttat ctccgacgat
421 attctcatca gtgaaatcca ggggaattct catgtttgac agcttatcat cgataagctt
481 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
541 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
601 gttatgccgg tactgccggg cctcttgcgg gataactgca cattcgggat atttctctat
661 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
721 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
781 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
841 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
901 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
961 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
1021 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
1081 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
1141 acgaggacaa gagtacgccg ctgcacgatc ttttggctcg tgtcgaacga gagctaccgg
1201 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
1261 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
1321 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
1381 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
1441 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
1501 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
1561 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
1621 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
1681 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
1741 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
1801 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
1861 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
1921 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
1981 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
2041 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
2101 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
2161 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
2221 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
2281 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
2341 tagatccggc ccgatgcgtc cggcgtagag gatctgaaga tcagcagttc aacctgttga
2401 tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac gtactaagct
2461 ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg gctaacgtac
2521 taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt aatataaatc
2581 agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa tatataaggc
2641 ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatgta acgcactgag
2701 aagcccttag agcctctcaa agcaattttc agtgacacag gaacacttaa cggctgacat
2761 gggaattcta ctgtttgggt gtatgagcta tttgcaaagg aaattggtga tgacaaagct
2821 cggcgctatt tgaagaaaat cgactatggc aacgccgatc ctttttgtcc ggtgttgggt
2881 tgaaggtgaa gccggtcggg gccgcagcgg gggccggctt ttcagccttg cccccctgct
2941 tcggccgccg tggctccggc gtcttgggtg ccggcgcggg ttccgcagcc ttggcctgcg
3001 gtgcgggcac atcggcgggc ttggccttga tgtgccgcct ggcgtgcgag cggaacgtct
3061 cgtaggagaa cttgaccttc cccgtttccc gcatgtgctc ccaaatggtg acgagcgcat
3121 agccggacgc taacgccgcc tcgacatccg ccctcaccgc caggaacgca accgcagcct
3181 catcacgccg gcgcttcttg gccgcgcggg attcaaccca ctcggccagc tcgtcggtgt
3241 agctctttgg catcgtctct cgcctgtccc ctcagttcag taatttcctg catttgcctg
3301 tttccagtcg gtagatattc cacaaaacag cagggaagca gcgcttttcc gctgcataac
3361 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
3421 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
3481 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
3541 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg
3601 taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac
3661 ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg
3721 ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa atcacgggcg
3781 tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg
3841 gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca
3901 cgatccgggc aacgttgttg ccattgctgc agttcactta caccgcttct caacccggta
3961 cgcaccataa aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca
4021 ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt
4081 aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg
4141 gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acagtctccg
4201 gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg ggcgtcttat
4261 gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga
4321 atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg
4381 gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc
4441 aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca
4501 ataagttgga gtcattacca aaaggttagg aatacggtta gccatttgcc tgcttttata
4561 tagttcatat gggattcacc tttatgttga taagaaataa aagaaaatgc caataggata
4621 tcggcatttt cttttgcgtt tttatttgtt aactgttaat tgtccttgtt caaggatgct
4681 gtctttgaca acagatgttt tcttgccttt gatgttcagc aggaagcttg gcgcaaacgt
4741 tgattgtttg tctgcgtaga atcctctgtt tgtcatatag cttgtaatca cgacattgtt
4801 tcctttcgct tgaggtacag cgaagtgtga gtaagtaaag gttacatcgt taggatcaag
4861 atccattttt aacacaaggc cagttttgtt cagcggcttg tatgggccag ttaaagaatt
4921 agaaacataa ccaagcatgt aaatatcgtt agacgtaatg ccgtcaatcg tcatttttga
4981 tccgcgggag tcagtgaaca ggtaccattt gccgttcatt ttaaagacgt tcgcgcgttc
5041 aatttcatct gttactgtgt tagatgcaat cagcggtttc atcacttttt tcagtgtgta
5101 atcatcgttt agctcaatca taccgagagc gccgtttgct aactcagccg tgcgtttttt
5161 atcgctttgc agaagttttt gactttcttg acggaagaat gatgtgcttt tgccatagta
5221 tgctttgtta aataaagatt cttcgccttg gtagccatct tcagttccag tgtttgcttc
5281 aaatactaag tatttgtggc ctttatcttc tacgtagtga ggatctctca gcgtatggtt
5341 gtcgcctgag ctgtagttgc cttcatcgat gaactgctgt acattttgat acgtttttcc
5401 gtcaccgtca aagattgatt tataatcctc tacaccgttg atgttcaaag agctgtctga
5461 tgctgatacg ttaacttgtg cagttgtcag tgtttgtttg ccgtaatgtt taccggagaa
5521 atcagtgtag aataaacgga tttttccgtc agatgtaaat gtggctgaac ctgaccattc
5581 ttgtgtttgg tcttttagga tagaatcatt tgcatcgaat ttgtcgctgt ctttaaagac
5641 gcggccagcg tttttccagc tgtcaataga agtttcgccg actttttgat agaacatgta
5701 aatcgatgtg tcatccgcat ttttaggatc tccggctaat gcaaagacga tgtggtagcc
5761 gtgatagttt gcgacagtgc cgtcagcgtt ttgtaatggc cagctgtccc aaacgtccag
5821 gccttttgca gaagagatat ttttaattgt ggacgaatcg aattcaggaa cttgatattt
5881 ttcatttttt tgctgttcag ggatttgcag catatcatgg cgtgtaatat gggaaatgcc
5941 gtatgtttcc ttatatggct tttggttcgt ttctttcgca aacgcttgag ttgcgcctcc
6001 tgccagcagt gcggtagtaa aggttaatac tgttgcttgt tttgcaaact ttttgatgtt
6061 catcgttcat gtctcctttt ttatgtactg tgttagcggt ctgcttcttc cagccctcct
6121 gtttgaagat ggcaagttag ttacgcacaa taaaaaaaga cctaaaatat gtaaggggtg
6181 acgccaaagt atacactttg ccctttacac attttaggtc ttgcctgctt tatcagtaac
6241 aaacccgcgc gatttacttt tcgacctcat tctattagac tctcgtttgg attgcaactg
6301 gtctattttc ctcttttgtt tgatagaaaa tcataaaagg atttgcagac tacgggccta
6361 aagaactaaa aaatctatct gtttcttttc attctctgta ttttttatag tttctgttgc
6421 atgggcataa agttgccttt ttaatcacaa ttcagaaaat atcataatat ctcatttcac
6481 taaataatag tgaacggcag gtatatgtga tgggttaaaa aggatcttaa ggcctaggtc
6541 tagcttggct gcaggggggg gggggggact tccattgttc attccacgga caaaaacaga
6601 gaaaggaaac gacagaggcc aaaaagcctc gctttcagca cctgtcgttt cctttctttt
6661 cagagggtat tttaaataaa aacattaagt tatgacgaag aagaacggaa acgccttaaa
6721 ccggaaaatt ttcataaata gcgaaaaccc gcgaggtcgc cgccccgtaa cctgtcggat
6781 caccggaaag gacccgtaaa gtgataatga ttatcatcta catatcacaa cgtgcgtgga
6841 ggccatcaaa ccacgtcaaa taatcaatta tgacgcaggt atcgtattaa ttgatctgca
6901 tcaacttaac gtaaaaacaa cttcagacaa tacaaatcag cgacactgaa tacggggcaa
6961 cctcatgtcc cccccccccc ctgcag
//