LOCUS LT726978 7025 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hTRIF, complete sequence.
ACCESSION LT726978
VERSION LT726978.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hTRIF
ORGANISM Mammalian expression vector pCAGGS-E-hTRIF
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7025)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7025
/organism="Mammalian expression vector pCAGGS-E-hTRIF"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5226."
/db_xref="taxon:1944785"
misc_feature 16..54
/note="E-tag"
CDS <64..2199
/codon_start=1
/transl_table=11
/note="hTRIF"
/protein_id="SJL87103.1"
/translation="ACTGPSLPSAFDILGAAGQDKLLYLKHKLKTPRPGCQGQDLLHA
MVLLKLGQETEARISLEALKADAVARLVARQWAGVDSTEDPEEPPDVSWAVARLYHLL
AEEKLCPASLRDVAYQEAVRTLSSRDDHRLGELQDEARNRCGWDIAGDPGSIRTLQSN
LGCLPPSSALPSGTRSLPRPIDGVSDWSQGCSLRSTGSPASLASNLEISQSPTMPFLS
LHRSPHGPSKLCDDPQASLVPEPVPGGCQEPEEMSWPPSGEIASPPELPSSPPPGLPE
VAPDATSTGLPDTPAAPETSTNYPVECTEGSAGPQSLPLPILEPVKNPCSVKDQTPLQ
LSVEDTTSPNTKPCPPTPTTPETSPPPPPPPPSSTPCSAHLTPSSLFPSSLESSSEQK
FYNFVILHARADEHIALRVREKLEALGVPDGATFCEDFQVPGRGELSCLQDAIDHSAF
IILLLTSNFDCRLSLHQVNQAMMSNLTRQGSPDCVIPFLPLESSPAQLSSDTASLLSG
LVRLDEHSQIFARKVANTFKPHRLQARKAMWRKEQDTRALREQSQHLDGERMQAAALN
AAYSAYLQSYLSYQAQMEQLQVAFGSHMSFGTGAPYGARMPFGGQVPLGAPPPFPTWP
GCPQPPPLHAWQAGTPPPPSPQPAAFPQSLPFPQSPAFPTASPAPPQSPGLQPLIIHH
AQMVQLGLNNHMWNQRGSQAPEDKTQEAE"
regulatory 2315..2406
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 2407..2763
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2841..2923)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 3065..3140
/note="SV40 ori"
regulatory 3171..3245
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3171..3245)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3360..3761)
/note="pMB1 ori"
CDS complement(4302..5162)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87104.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5298..5675
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5681..7022
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 6050..6974
/note="intron AG promoter"
BASE COUNT 1430 a 2089 c 1945 g 1561 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctgcctgca caggcccatc acttcctagc gccttcgaca ttctaggtgc agcaggccag
121 gacaagctct tgtatctgaa gcacaaactg aagaccccac gcccaggctg ccaggggcag
181 gacctcctgc atgccatggt tctcctgaag ctgggccagg aaactgaggc caggatctct
241 ctagaggcat tgaaggccga tgcggtggcc cggctggtgg cccgccagtg ggctggcgtg
301 gacagcaccg aggacccaga ggagccccca gatgtgtcct gggctgtggc ccgcttgtac
361 cacctgctgg ctgaggagaa gctgtgcccc gcctcgctgc gggacgtggc ctaccaggaa
421 gccgtccgca ccctcagctc cagggacgac caccggctgg gggaacttca ggatgaggcc
481 cgaaaccggt gtgggtggga cattgctggg gatccaggga gcatccggac gctccagtcc
541 aatctgggct gcctcccacc atcctcggct ttgccctctg ggaccaggag cctcccacgc
601 cccattgacg gtgtttcgga ctggagccaa gggtgctccc tgcgatccac tggcagccct
661 gcctccctgg ccagcaactt ggaaatcagc cagtccccta ccatgccctt cctcagcctg
721 caccgcagcc cacatgggcc cagcaagctc tgtgacgacc cccaggccag cttggtgccc
781 gagcctgtcc ccggtggctg ccaggagcct gaggagatga gctggccgcc atcgggggag
841 attgccagcc caccagagct gccaagcagc ccacctcctg ggcttcccga agtggcccca
901 gatgcaacct ccactggcct ccctgatacc cccgcagctc cagaaaccag caccaactac
961 ccagtggagt gcaccgaggg gtctgcaggc ccccagtctc tccccttgcc tattctggag
1021 ccggtcaaaa acccctgctc tgtcaaagac cagacgccac tccaactttc tgtagaagat
1081 accacctctc caaataccaa gccgtgccca cctactccca ccaccccaga aacatcccct
1141 cctcctcctc ctcctcctcc ttcatctact ccttgttcag ctcacctgac cccctcctcc
1201 ctgttccctt cctccctgga atcatcatcg gaacagaaat tctataactt tgtgatcctc
1261 cacgccaggg cagacgaaca catcgccctg cgggttcggg agaagctgga ggcccttggc
1321 gtgcccgacg gggccacctt ctgcgaggat ttccaggtgc cggggcgcgg ggagctgagc
1381 tgcctgcagg acgccataga ccactcagct ttcatcatcc tacttctcac ctccaacttc
1441 gactgtcgcc tgagcctgca ccaggtgaac caagccatga tgagcaacct cacgcgacag
1501 gggtcgccag actgtgtcat ccccttcctg cccctggaga gctccccggc ccagctcagc
1561 tccgacacgg ccagcctgct ctccgggctg gtgcggctgg acgaacactc ccagatcttc
1621 gccaggaagg tggccaacac cttcaagccc cacaggcttc aggcccgaaa ggccatgtgg
1681 aggaaggaac aggacacccg agccctgcgg gaacagagcc aacacctgga cggtgagcgg
1741 atgcaggcgg cggcactgaa cgcagcctac tcagcctacc tccagagcta cttgtcctac
1801 caggcacaga tggagcagct ccaggtggct tttgggagcc acatgtcatt tgggactggg
1861 gcgccctatg gggctcgaat gccctttggg ggccaggtgc ccctgggagc cccgccaccc
1921 tttcccactt ggccggggtg cccgcagccg ccacccctgc acgcatggca ggctggcacc
1981 cccccaccgc cctccccaca gccagcagcc tttccacagt cactgccctt cccgcagtcc
2041 ccagccttcc ctacggcctc acccgcaccc cctcagagcc cagggctgca acccctcatt
2101 atccaccacg cacagatggt acagctgggg ctgaacaacc acatgtggaa ccagagaggg
2161 tcccaggcgc ccgaggacaa gacgcaggag gcagaatgac ccgggacatt aaacgcgtgg
2221 gtaccggatc cggcaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
2281 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
2341 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
2401 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
2461 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
2521 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
2581 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
2641 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
2701 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
2761 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
2821 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
2881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
2941 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
3001 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
3121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
3181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
3241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
3301 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
3361 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
3421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag
3481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
3541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
3601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
3661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
3721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
3781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
3841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
3901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
3961 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
4021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
4081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
4141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
4201 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
4261 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
4321 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
4381 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
4441 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
4501 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
4561 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
4621 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
4681 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
4741 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
4801 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
4861 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
4921 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
4981 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
5041 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
5101 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
5161 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
5221 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
5281 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
5341 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
5401 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
5461 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
5521 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
5581 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
5641 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
5701 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
5761 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg
5821 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg
5881 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
5941 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc
6001 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
6061 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
6121 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
6181 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
6241 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
6301 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
6361 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
6421 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
6481 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
6541 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg
6601 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
6661 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
6721 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
6781 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
6841 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
6901 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
6961 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
7021 aagaa
//