LOCUS LT726976 6218 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pPTB-HcRED, complete sequence.
ACCESSION LT726976
VERSION LT726976.1
KEYWORDS .
SOURCE Mammalian expression vector pPTB-HcRED
ORGANISM Mammalian expression vector pPTB-HcRED
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6218)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6218
/organism="Mammalian expression vector pPTB-HcRED"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5218."
/db_xref="taxon:1945054"
regulatory 1..568
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
CDS 639..>2309
/transl_table=11
/note="hPTB"
/protein_id="SJL87097.1"
/translation="MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDS
KKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMN
TEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSG
NLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTK
NNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYT
RPDLPSGDSQPSLDQTMAAAFGAPGIISASPYAGAGFPPTFAIPQAAGLSVPNVHGAL
APLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQR
VKILFNKKENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQ
GLTKDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLFSSNGGVVK
GFKFFQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKSTI"
CDS 2316..3002
/transl_table=11
/note="HcRed1"
/protein_id="SJL87098.1"
/translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRI
HVTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGI
LTAHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNV
MALKVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVA
RYSDLPEKAN"
rep_origin complement(3134..3589)
/note="f1 ori"
regulatory 3590..3685
/note="amp promoter"
/regulatory_class="promoter"
misc_feature 3686..3720
/note="5' UTR of amp, incl. RBS"
regulatory 3741..4022
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3979..4054
/note="SV40 ori"
CDS 4114..4908
/transl_table=11
/note="neomycin"
/protein_id="SJL87099.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5099..5168
/note="HSV-TK polyA"
/regulatory_class="terminator"
rep_origin 5886..6212
/note="pMB1 ori"
BASE COUNT 1440 a 1787 c 1680 g 1311 t
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attccaccat ggacggcatt gtcccagata
661 tagccgttgg tacaaagcgg ggatctgacg agcttttctc tacttgtgtc actaacggac
721 cgtttatcat gagcagcaac tcggcttctg cagcaaacgg aaatgacagc aagaagttca
781 aaggtgacag ccgaagtgca ggcgtcccct ctagagtgat ccacatccgg aagctcccca
841 tcgacgtcac ggagggggaa gtcatctccc tggggctgcc ctttgggaag gtcaccaacc
901 tcctgatgct gaaggggaaa aaccaggcct tcatcgagat gaacacggag gaggctgcca
961 acaccatggt gaactactac acctcggtga cccctgtgct gcgcggccag cccatctaca
1021 tccagttctc caaccacaag gagctgaaga ccgacagctc tcccaaccag gcgcgggccc
1081 aggcggccct gcaggcggtg aactcggtcc agtcggggaa cctggccttg gctgcctcgg
1141 cggcggccgt ggacgcaggg atggcgatgg ccgggcagag ccccgtgctc aggatcatcg
1201 tggagaacct cttctaccct gtgaccctgg atgtgctgca ccagattttc tccaagttcg
1261 gcacagtgtt gaagatcatc accttcacca agaacaacca gttccaggcc ctgctgcagt
1321 atgcggaccc cgtgagcgcc cagcacgcca agctgtcgct ggacgggcag aacatctaca
1381 acgcctgctg cacgctgcgc atcgactttt ccaagctcac cagcctcaac gtcaagtaca
1441 acaatgacaa gagccgtgac tacacacgcc cagacctgcc ttccggggac agccagccct
1501 cgctggacca gaccatggcc gcggccttcg gtgcacctgg tataatctca gcctctccgt
1561 atgcaggagc tggtttccct cccacctttg ccattcctca agctgcaggc ctttccgttc
1621 cgaacgtcca cggcgccctg gcccccctgg ccatcccctc ggcggcggcg gcagctgcgg
1681 cggcaggtcg gatcgccatc ccgggcctgg cgggggcagg aaattctgta ttgctggtca
1741 gcaacctcaa cccagagaga gtcacacccc aaagcctctt tattcttttc ggcgtctacg
1801 gtgacgtgca gcgcgtgaag atcctgttca ataagaagga gaacgcccta gtgcagatgg
1861 cggacggcaa ccaggcccag ctggccatga gccacctgaa cgggcacaag ctgcacggga
1921 agcccatccg catcacgctc tcgaagcacc agaacgtgca gctgccccgc gagggccagg
1981 aggaccaggg cctgaccaag gactacggca actcacccct gcaccgcttc aagaagccgg
2041 gctccaagaa cttccagaac atattcccgc cctcggccac gctgcacctc tccaacatcc
2101 cgccctcagt ctccgaggag gatctcaagg tcctgttttc cagcaatggg ggcgtcgtca
2161 aaggattcaa gttcttccag aaggaccgca agatggcact gatccagatg ggctccgtgg
2221 aggaggcggt ccaggccctc attgacctgc acaaccacga cctcggggag aaccaccacc
2281 tgcgggtctc cttctccaag tccaccatcg gatccatggt gagcggcctg ctgaaggaga
2341 gtatgcgcat caagatgtac atggagggca ccgtgaacgg ccactacttc aagtgcgagg
2401 gcgagggcga cggcaacccc ttcgccggca cccagagcat gagaatccac gtgaccgagg
2461 gcgcccccct gcccttcgcc ttcgacatcc tggccccctg ctgcgagtac ggcagcagga
2521 ccttcgtgca ccacaccgcc gagatccccg acttcttcaa gcagagcttc cccgagggct
2581 tcacctggga gagaaccacc acctacgagg acggcggcat cctgaccgcc caccaggaca
2641 ccagcctgga gggcaactgc ctgatctaca aggtgaaggt gcacggcacc aacttccccg
2701 ccgacggccc cgtgatgaag aacaagagcg gcggctggga gcccagcacc gaggtggtgt
2761 accccgagaa cggcgtgctg tgcggccgga acgtgatggc cctgaaggtg ggcgaccggc
2821 acctgatctg ccaccactac accagctacc ggagcaagaa ggccgtgcgc gccctgacca
2881 tgcccggctt ccacttcacc gacatccggc tccagatgct gcggaagaag aaggacgagt
2941 acttcgagct gtacgaggcc agcgtggccc ggtacagcga cctgcccgag aaggccaact
3001 gagttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
3061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
3121 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
3181 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
3241 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
3301 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
3361 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
3421 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
3481 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
3541 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
3601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
3661 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
3721 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
3781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
3841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
3901 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
3961 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
4021 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
4081 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
4141 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
4201 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
4261 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
4321 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
4381 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
4441 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
4501 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
4561 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
4621 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
4681 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
4741 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
4801 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
4861 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
4921 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
4981 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
5041 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
5101 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
5161 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
5221 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
5281 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
5341 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
5401 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
5461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
5521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
5581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
5641 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
5701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
5761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
5821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
5881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
5941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
6001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
6061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
6121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
6181 cccctgattc tgtggataac cgtattaccg ccatgcat
//