LOCUS LT726974 4818 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGST7, complete sequence.
ACCESSION LT726974
VERSION LT726974.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGST7
ORGANISM Mammalian expression vector pCAGGST7
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4818)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4818
/organism="Mammalian expression vector pCAGGST7"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5216."
/db_xref="taxon:1944896"
regulatory 1..17
/note="T7 promoter"
/regulatory_class="promoter"
regulatory 108..199
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 200..556
/note="3' FR of beta-globin"
regulatory complement(634..716)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 858..933
/note="SV40 ori"
regulatory complement(964..1038)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 964..1038
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1153..1554)
/note="pMB1 ori"
CDS complement(2095..2955)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87094.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3091..3468
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3474..4815
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 3843..4767
/note="intron AG promoter"
BASE COUNT 1010 a 1275 c 1341 g 1192 t
ORIGIN
1 taatacgact cactataggg ctcgaggaat tcactcctca ggtgcaggct gcctatcaga
61 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
121 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
181 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
241 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
301 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
361 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
421 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
481 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
541 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
601 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
661 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
721 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
781 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
841 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
961 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
1021 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
1081 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
1141 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
1201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga
1261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
1321 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
1381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
1441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
1501 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
1561 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
1621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
1681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
1741 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
1801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
1861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
1921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
1981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
2041 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
2101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
2161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
2221 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
2281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
2341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
2401 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
2461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
2521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
2581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
2641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
2701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
2761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
2821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
2881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
2941 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
3001 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
3061 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
3121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
3181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
3241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
3301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
3361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
3421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
3481 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
3541 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc
3601 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag
3661 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
3721 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc
3781 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
3841 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
3901 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
3961 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
4021 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
4081 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
4141 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
4201 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
4261 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
4321 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
4381 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
4441 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
4501 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
4561 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
4621 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
4681 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
4741 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
4801 catcattttg gcaaagaa
//