LOCUS LT726973 5340 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-deltaPTB, complete sequence.
ACCESSION LT726973
VERSION LT726973.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-deltaPTB
ORGANISM Mammalian expression vector pCAGGS-deltaPTB
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5340)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5340
/organism="Mammalian expression vector pCAGGS-deltaPTB"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5215."
/db_xref="taxon:1944758"
CDS 8..628
/transl_table=11
/note="deltaPTB"
/protein_id="SJL87092.1"
/translation="MVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAF
IEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNS
VQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKII
TFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKL"
misc_feature 20..274
/note="RRM1"
misc_feature 395..625
/note="RRM2"
regulatory 630..721
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 722..1078
/note="3' FR of beta-globin"
regulatory complement(1156..1238)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1380..1455
/note="SV40 ori"
regulatory complement(1486..1560)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1486..1560
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1675..2076)
/note="pMB1 ori"
CDS complement(2617..3477)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87093.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3613..3990
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3996..5337
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4365..5289
/note="intron AG promoter"
BASE COUNT 1117 a 1455 c 1488 g 1280 t
ORIGIN
1 ttccaccatg gtcccctcta gagtgatcca catccggaag ctccccatcg acgtcacgga
61 gggggaagtc atctccctgg ggctgccctt tgggaaggtc accaacctcc tgatgctgaa
121 ggggaaaaac caggccttca tcgagatgaa cacggaggag gctgccaaca ccatggtgaa
181 ctactacacc tcggtgaccc ctgtgctgcg cggccagccc atctacatcc agttctccaa
241 ccacaaggag ctgaagaccg acagctctcc caaccaggcg cgggcccagg cggccctgca
301 ggcggtgaac tcggtccagt cggggaacct ggccttggct gcctcggcgg cggccgtgga
361 cgcagggatg gcgatggccg ggcagagccc cgtgctcagg atcatcgtgg agaacctctt
421 ctaccctgtg accctggatg tgctgcacca gattttctcc aagttcggca cagtgttgaa
481 gatcatcacc ttcaccaaga acaaccagtt ccaggccctg ctgcagtatg cggaccccgt
541 gagcgcccag cacgccaagc tgtcgctgga cgggcagaac atctacaacg cctgctgcac
601 gctgcgcatc gacttttcca agctctagag atctttttcc ctctgccaaa aattatgggg
661 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
721 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
781 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
841 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
901 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
961 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
1021 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
1081 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
1141 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg
1201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
1261 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
1321 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
1381 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
1441 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
1621 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
1681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
1741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat
1801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
1861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
1921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
1981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
2041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
2101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
2161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
2221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
2641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
2701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
2761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
2821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
2881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
2941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
3001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
3061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
3121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
3181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
3241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
3421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
3481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
3541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
3601 gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
3661 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
3721 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
3781 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
3841 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
3901 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
3961 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
4021 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
4081 tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg
4141 gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc
4201 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
4261 cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct
4321 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
4381 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
4441 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
4501 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
4561 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
4621 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
4681 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
4741 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
4801 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
4861 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc
4921 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
4981 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
5041 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
5101 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
5161 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
5221 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
5281 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//