LOCUS LT726962 6021 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV-Sport-di-FR-HIFdel1, complete
sequence.
ACCESSION LT726962
VERSION LT726962.1
KEYWORDS .
SOURCE Mammalian expression vector pSV-Sport-di-FR-HIFdel1
ORGANISM Mammalian expression vector pSV-Sport-di-FR-HIFdel1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6021)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6021
/organism="Mammalian expression vector
pSV-Sport-di-FR-HIFdel1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5203."
/db_xref="taxon:1945143"
CDS 83..1735
/transl_table=11
/note="LUCm"
/protein_id="SJL87067.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
misc_feature 1743..1932
/note="HIFdel1"
CDS 1933..2868
/transl_table=11
/note="rLUC"
/protein_id="SJL87068.1"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
regulatory complement(2960..2976)
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 3065..3104
/note="intron SV40 small t-antigen"
regulatory complement(3341..3437)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 3341..3437
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(3550..3952)
/note="pMB1 ori"
CDS complement(4483..5343)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87069.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5539..5596
/note="lacUV5 promoter"
/regulatory_class="promoter"
regulatory 5641..5922
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 5879..5954
/note="SV40 ori"
regulatory 5985..6001
/note="SP6 promoter"
/regulatory_class="promoter"
BASE COUNT 1621 a 1380 c 1421 g 1599 t
ORIGIN
1 cgagctctta cgcgtgctag cccgggctcg agatctgcga tctaagtaag cttggcattc
61 cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc
121 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
181 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
241 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
301 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
361 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
421 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
481 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
541 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
601 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
661 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
721 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
781 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
841 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
901 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
961 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
1021 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
1081 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
1141 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
1201 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
1261 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
1321 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
1381 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
1441 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
1501 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
1561 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
1621 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
1681 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta
1741 gacctaggaa cccgagccgg agctcagcga gcgcagcctg cagctcccgc ctcgccgtcc
1801 cggggggcgt cccgcctccc accccgcctc tggacttgtc tctttctccg cgcgcgcgga
1861 cagagcgggc gcttaggccc gagcgagccc gggggccgcc ggccgggaag acaacgcggg
1921 caccgattcg ccatggcttc gaaagtttat gatccagaac aaaggaaacg gatgataact
1981 ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc ttgattcatt tattaattat
2041 tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct
2101 tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca
2161 gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag gttacttgat
2221 cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt
2281 gtcggccatg attggggtgc ttgtttggca tttcattata gctatgagca tcaagataag
2341 atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg
2401 cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa aatggttttg
2461 gagaataact tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca
2521 gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca
2581 ttatcatggc ctcgtgaaat cccgttagta aaaggtggta aacctgacgt tgtacaaatt
2641 gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat gtttattgaa
2701 tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt tcctaatact
2761 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
2821 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataaga attcccgggt
2881 cgacgagctc actagtcggc ggccgctcta gaggatccaa gcttacgtac gcgtgcatgc
2941 gacgtcatag ctctctccct atagtgagtc gtattataag ctagcttggg atctttgtga
3001 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
3061 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actagctgca tatgcttgct
3121 gcttgagagt tttgcttact gagtatgatt tatgaaaata ttatacacag gagctagtga
3181 ttctaattgt ttgtgtattt tagattcaca gtcccaaggc tcatttcagg cccctcagtc
3241 ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
3301 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
3361 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
3421 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
3481 cttatcatgt ctggatcctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
3541 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
3661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
3721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
3781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
3841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
3901 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
3961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
4021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
4081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
4141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcggccagt
4201 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
4261 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
4321 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
4381 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
4441 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
4501 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
4561 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
4621 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
4681 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
4741 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
4801 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
4861 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
4921 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
4981 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
5041 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
5101 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
5161 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
5221 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
5281 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
5341 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
5401 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
5461 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
5521 gcgtatacga ggccctttca ctcattaggc accccaggct ttacacttta tagcttccgg
5581 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agcatcgatg
5641 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
5701 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
5761 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
5821 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta
5881 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
5941 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat
6001 agaaggtacg cctgcaggta c
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