LOCUS LT726956 4864 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pGL3casp11p-Del6, complete sequence.
ACCESSION LT726956
VERSION LT726956.1
KEYWORDS .
SOURCE Reporter vector pGL3casp11p-Del6
ORGANISM Reporter vector pGL3casp11p-Del6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4864)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4864
/organism="Reporter vector pGL3casp11p-Del6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5186."
/db_xref="taxon:1945214"
regulatory 21..131
/note="86 bp mCASP-11 promoter fragm."
/regulatory_class="promoter"
CDS 134..1786
/transl_table=11
/note="LUCm"
/protein_id="SJL87054.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 1874..1970
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(1874..1970)
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2183..2585)
/note="pMB1 ori"
CDS complement(3126..3986)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87055.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 4118..4573
/note="f1 ori"
regulatory 4704..4857
/note="synthetic polyA"
/regulatory_class="terminator"
BASE COUNT 1265 a 1162 c 1161 g 1276 t
ORIGIN
1 ggtaccgagc tcttacgcgt agcagctctt tcaacatctc ctggaagtcc ccgcctccct
61 atgctaagga aatgcccagt aactttcatt ttactctgtc aagctgtctt cacagtgcga
121 aagaactgag gccatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
181 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
241 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
301 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
361 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
421 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
481 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
541 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
601 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
661 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
721 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
781 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
841 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
901 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag
961 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc
1021 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg
1081 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat
1141 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
1201 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
1261 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat
1321 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
1381 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga
1441 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc
1501 catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga
1561 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
1621 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
1681 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
1741 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg
1801 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
1861 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
1921 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
1981 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
2041 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
2101 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
2161 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
2221 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
2281 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
2341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
2401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
2461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
2521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
2581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
2641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
2701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
2761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
2821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
2881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
2941 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
3001 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
3061 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
3121 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
3181 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
3241 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
3301 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
3361 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
3421 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
3481 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
3541 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
3601 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
3661 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
3721 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
3781 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
3841 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
3901 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
3961 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
4021 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
4081 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
4141 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
4201 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
4261 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
4321 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
4381 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
4441 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
4501 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
4561 acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
4621 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta
4681 cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
4741 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
4801 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc
4861 gata
//