LOCUS LT726953 4932 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pGL3casp11p-Del3, complete sequence.
ACCESSION LT726953
VERSION LT726953.1
KEYWORDS .
SOURCE Reporter vector pGL3casp11p-Del3
ORGANISM Reporter vector pGL3casp11p-Del3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4932)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4932
/organism="Reporter vector pGL3casp11p-Del3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5183."
/db_xref="taxon:1945211"
regulatory 21..174
/note="154 bp mCASP-11 promoter fragment"
/regulatory_class="promoter"
misc_feature 175..199
/note="5' UTR of mCASP-11"
CDS 202..1854
/transl_table=11
/note="LUCm"
/protein_id="SJL87048.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 1942..2038
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(1942..2038)
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2251..2653)
/note="pMB1 ori"
CDS complement(3194..4054)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87049.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 4186..4641
/note="f1 ori"
regulatory 4772..4925
/note="synthetic polyA"
/regulatory_class="terminator"
BASE COUNT 1284 a 1174 c 1179 g 1295 t
ORIGIN
1 ggtaccgagc tcttacgcgt aattcaggaa aagggaggcc agttttatga gttacagggg
61 atttccccac agcagttgat actttctgag cagctctttc aacatctcct ggaagtcccc
121 gcctccctat gctaaggaaa tgcccagtaa ctttcatttt actctgtcaa gctgtcttca
181 cagtgcgaaa gaactgaggc catggaagac gccaaaaaca taaagaaagg cccggcgcca
241 ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
301 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac
361 gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
421 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
481 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
541 ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg
601 caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat
661 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
721 cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca
781 ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga
841 actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
901 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
961 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
1021 ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc
1081 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
1141 tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg
1201 ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct gattacaccc
1261 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
1321 gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga
1381 ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
1441 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
1501 atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa
1561 ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc
1621 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
1681 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
1741 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
1801 aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag
1861 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
1921 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
1981 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
2041 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
2101 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
2161 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
2221 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
2281 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
2341 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
2401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
2641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
2761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
2821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
2881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
2941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3061 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
3241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
3301 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
3361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
3421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
3481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
3541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
3601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
3661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
3721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
3781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
3841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
3901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
3961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
4021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
4081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4141 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
4201 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
4261 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
4321 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
4381 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
4441 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
4501 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
4561 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
4621 aaatattaac gtttacaatt tcccattcgc cattcaggct gcgcaactgt tgggaagggc
4681 gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata
4741 ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct
4801 gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac
4861 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt
4921 tctctatcga ta
//