LOCUS LT726949 5000 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-FLAG-hUb-K27, complete sequence. ACCESSION LT726949 VERSION LT726949.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-FLAG-hUb-K27 ORGANISM Mammalian expression vector pCAGGS-FLAG-hUb-K27 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5000) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5000 /organism="Mammalian expression vector pCAGGS-FLAG-hUb-K27" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5896." /db_xref="taxon:1944835" misc_feature 13..36 /note="FLAG epitope" CDS 46..276 /transl_table=11 /note="hUBB mutated mature sequence" /protein_id="SJL87040.1" /translation="MQIFVRTLTGRTITLEVEPSDTIENVKARIQDREGIPPDQQRLI FAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG" misc_feature 61..63 /note="K6R" misc_feature 76..78 /note="K11R" misc_feature 130..132 /note="K29R" misc_feature 142..144 /note="K33R" misc_feature 187..189 /note="K48R" misc_feature 232..234 /note="K63R" regulatory 286..377 /note="3' UTR of rabbit beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 378..734 /note="3' flanking region of rabbit beta-globin" regulatory complement(812..894) /note="lac promoter" /regulatory_class="promoter" rep_origin 1036..1111 /note="SV40 ori" regulatory complement(1142..1216) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 1142..1216 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(1331..1732) /note="pMB1 ori" CDS complement(2273..3133) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87041.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3273..3650 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 3656..4997 /note="AG promoter" /regulatory_class="promoter" misc_feature 4025..4949 /note="intron AG promoter" BASE COUNT 1058 a 1327 c 1398 g 1217 t ORIGIN 1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgcgatgca gatcttcgtg 61 aggaccctga ccggcaggac catcactctg gaggtggagc ccagtgacac catcgaaaat 121 gtgaaggcca gaatccaaga tagggaaggc atcccccccg accagcagag gctcatcttt 181 gcaggcaggc agctggaaga tggccgcact ctttctgact acaacatcca gagggagtcg 241 accctgcacc tggtcctgcg tctcagaggt gggtaggtcg acgaagatct ttttccctct 301 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg 361 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca 421 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca 481 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg 541 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt 601 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat 661 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt 721 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt 781 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 841 gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 901 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa 961 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 1021 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 1081 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 1141 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 1201 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 1261 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg 1321 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 1381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata 1441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 1981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 2041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 2101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 2161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 2221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 2281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 2341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 2401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 2461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 2521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 2581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 2641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 2701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 2761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 2821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 2881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 2941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 3001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 3061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 3121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 3181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 3241 catttccccg aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata 3301 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 3361 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 3421 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 3481 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 3541 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 3601 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt 3661 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta 3721 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc 3781 cccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc 3841 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg 3901 gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt 3961 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc 4021 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg 4081 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt 4141 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg 4201 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct 4261 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg 4321 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg 4381 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc 4441 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg 4501 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg 4561 ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc 4621 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg 4681 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt 4741 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc 4801 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac 4861 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc 4921 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt 4981 ctcatcattt tggcaaagaa //