LOCUS LT726946 4708 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLc28LIG8, complete sequence.
ACCESSION LT726946
VERSION LT726946.1
KEYWORDS .
SOURCE Bacterial expression vector pPLc28LIG8
ORGANISM Bacterial expression vector pPLc28LIG8
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4708)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4708
/organism="Bacterial expression vector pPLc28LIG8"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 574."
/db_xref="taxon:1944698"
misc_feature 18..537
/note="5' UTR of phage T4 ligase, incl. RBS"
CDS 538..2001
/transl_table=11
/note="phage T4 ligase"
/protein_id="SJL87034.1"
/translation="MILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQ
YYIKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDV
EVLRRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKA
DGARCFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGEL
VYHEQVKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQC
MKFQVWDYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVI
YKKYIDQGLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGF
ILESECGKIKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGR
TDYVKLFLPIAIRLREDKTKANTFEDVFGDFHEVTGL"
rep_origin complement(2293..2695)
/note="pMB1 ori"
CDS complement(3236..4096)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87035.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4462..4592
/note="PL promoter"
/regulatory_class="promoter"
BASE COUNT 1386 a 987 c 1072 g 1263 t
ORIGIN
1 ttccggatcc ggccaagctt ttttcaccaa aggaaaataa agaaagtttt gatagtgaaa
61 aagctataaa agatgtaatt gaaaagatga ttaaaaacga ttcttttcct tggaacacta
121 cctggagaac tcctggattt aatccttata atccatatca tccatatcag acgcatccgt
181 ttcagacatg gaactatatt aagcccggcg gtatagagta tttgtataat agacctactt
241 gtggtgataa tattttccaa ggagcattct aatgtttgtt gttcacacta tttatgaaaa
301 tgaaggtaat actacacgtg attacggtca cgtaaatcaa ttttttagat gcaatccaga
361 attccgagct caaaaagacg aacgaatttt taaaaaatgt gtagagcaag gtttcattta
421 tatcaagcac tggatgcaag gaaataaagt tagaaccacg taccacaggt ctttgactga
481 gcttaatgat gaattgattt ataatagagc tgtaaaccaa actctaaagg atgaacaatg
541 attcttaaaa ttctgaacga aatagcatct attggttcaa ctaaacagaa gcaagcaatt
601 cttgaaaaga ataaagataa tgaattgctt aaacgagtat atcgtctgac ttattctcgt
661 gggttacagt attatatcaa gaaatggcct aaacctggta ttgctaccca gagttttgga
721 atgttgactc ttaccgatat gcttgacttc attgaattca cattagctac tcggaaattg
781 actggaaatg cagcaattga ggaattaact ggatatatca ccgatggtaa aaaagatgat
841 gttgaagttt tgcgtcgagt gatgatgcga gaccttgaat gtggtgcttc agtatctatt
901 gcaaacaaag tttggccagg tttaattcct gaacaacctc aaatgctcgc aagttcttat
961 gatgaaaaag gcattaataa gaatatcaaa tttccagcct ttgctcagtt aaaagctgat
1021 ggagctcggt gttttgctga agttagaggt gatgaattag atgatgttcg tcttttatca
1081 cgagctggta atgaatatct aggattagat cttcttaagg aagagttaat taaaatgacc
1141 gctgaagccc gccagattca tccagaaggt gtgttgattg atggcgaatt ggtataccat
1201 gagcaagtta aaaaggagcc agaaggccta gattttcttt ttgatgctta tcctgaaaac
1261 agtaaagcta aagaattcgc cgaagtagct gaatcacgta ctgcttctaa tggaatcgcc
1321 aataaatctt taaagggaac catttctgaa aaagaagcac aatgcatgaa gtttcaggtc
1381 tgggattatg tcccgttggt agaaatatac agtcttcctg catttcgttt gaaatatgat
1441 gtacgttttt ctaaactaga acaaatgaca tctggatatg ataaagtaat tttaattgaa
1501 aaccaggtag taaataacct agatgaagct aaggtaattt ataaaaagta tattgaccaa
1561 ggtcttgaag gtattattct caaaaatatc gatggattat gggaaaatgc tcgttcaaaa
1621 aatctttata aatttaaaga agtaattgat gttgatttaa aaattgtagg aatttatcct
1681 caccgtaaag accctactaa agcgggtgga tttattcttg agtcagagtg tggaaaaatt
1741 aaggtaaatg ctggttcagg cttaaaagat aaagccggtg taaaatcgca tgaacttgac
1801 cgtactcgca ttatggaaaa ccaaaattat tatattggaa aaattctaga gtgcgaatgc
1861 aacggttggt taaaatctga tggccgcact gattacgtta aattatttct tccgattgcg
1921 attcgtttac gtgaagataa aactaaagct aatacattcg aagatgtatt tggtgatttt
1981 catgaggtaa ctggtctatg aaagcttggc tgcctcgcgc gtttcggtga tgacggtgaa
2041 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
2101 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
2161 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
2221 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
2281 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
2341 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
2401 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
2461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
2521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
2581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
2641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
2701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
2761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
2821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
2881 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
2941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
3001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
3061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
3121 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
3181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
3241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
3301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
3361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
3421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
3481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
3541 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
3601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
3661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
3721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
3781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
3841 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
3901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
3961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
4021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
4081 aatgttgaat actcatactc ttcctttttc aatattatgt aagcagacag ttttattgtt
4141 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
4201 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
4261 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
4321 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
4381 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgccggtg atgccggcca
4441 cgatgcgtcc ggcgtagagg atctctcacc taccaaacaa tgcccccctg caaaaaataa
4501 attcatataa aaaacataca gataaccatc tgcggtgata aattatctct ggcggtgttg
4561 acataaatac cactggcggt gatactgagc acatcagcag gacgcactga ccaccatgaa
4621 ggtgacgctc ttaaaattaa gccctgaaga agggcagcat tcaaagcaga aggctttggg
4681 gtgtgtgata cgaaacgaag cattggaa
//