LOCUS LT726944 7370 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMSCV-F-BirA, complete sequence.
ACCESSION LT726944
VERSION LT726944.1
KEYWORDS .
SOURCE Vector pMSCV-F-BirA
ORGANISM Vector pMSCV-F-BirA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7370)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7370
/organism="Vector pMSCV-F-BirA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5689."
/db_xref="taxon:1945353"
regulatory 1..513
/note="5' MSCV-PCMV LTR"
/regulatory_class="promoter"
misc_feature 516..1404
/note="psi+ extended packaging signal"
misc_feature 1443..1466
/note="FLAG epitope"
CDS 1479..2444
/transl_table=11
/note="birA"
/protein_id="SJL87030.1"
/translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
DWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
SGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
regulatory 2506..3013
/note="mPGK1 promoter"
/regulatory_class="promoter"
CDS 3033..3632
/transl_table=11
/note="puromycin resistance"
/protein_id="SJL87031.1"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
regulatory 3733..4245
/note="3' MSCV-PCMV LTR"
/regulatory_class="terminator"
regulatory complement(4458..4541)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4633..5035)
/note="pMB1 ori"
CDS complement(5576..6436)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87032.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1687 a 2011 c 1986 g 1686 t
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaaactt aagcttacca
1441 tggattacaa ggacgatgac gataaggtac ccggatccat gaaggataac accgtgccac
1501 tgaaattgat tgccctgtta gcgaacggtg aatttcactc tggcgagcag ttgggtgaaa
1561 cgctgggaat gagccgggcg gctattaata aacacattca gacactgcgt gactggggcg
1621 ttgatgtctt taccgttccg ggtaaaggat acagcctgcc tgagcctatc cagttactta
1681 atgctaaaca gatattgggt cagctggatg gcggtagtgt agccgtgctg ccagtgattg
1741 actccacgaa tcagtacctt cttgatcgta tcggagagct taaatcgggc gatgcttgca
1801 ttgcagaata ccagcaggct ggccgtggtc gccggggtcg gaaatggttt tcgccttttg
1861 gcgcaaactt atatttgtcg atgttctggc gtctggaaca aggcccggcg gcggcgattg
1921 gtttaagtct ggttatcggt atcgtgatgg cggaagtatt acgcaagctg ggtgcagata
1981 aagttcgtgt taaatggcct aatgacctct atctgcagga tcgcaagctg gcaggcattc
2041 tggtggagct gactggcaaa actggcgatg cggcgcaaat agtcattgga gccgggatca
2101 acatggcaat gcgccgtgtt gaagagagtg tcgttaatca ggggtggatc acgctgcagg
2161 aagcggggat caatctcgat cgtaatacgt tggcggccat gctaatacgt gaattacgtg
2221 ctgcgttgga actcttcgaa caagaaggat tggcacctta tctgtcgcgc tgggaaaagc
2281 tggataattt tattaatcgc ccagtgaaac ttatcattgg tgataaagaa atatttggca
2341 tttcacgcgg aatagacaaa cagggggctt tattacttga gcaggatgga ataataaaac
2401 cctggatggg cggtgaaata tccctgcgta gtgcagaaaa ataagaattc tgcagatatc
2461 cagcacagtg gcggccgctc gagtctagag ggcccgttta acgaattcta ccgggtaggg
2521 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
2581 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
2641 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
2701 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
2761 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
2821 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
2881 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
2941 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
3001 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc
3061 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
3121 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
3181 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
3241 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
3301 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
3361 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
3421 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
3481 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
3541 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
3601 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
3661 ccgaaaggag cgcacgaccc catgcatcga taaaataaaa gattttattt agtctccaga
3721 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
3781 tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac
3841 agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
3901 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac
3961 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
4021 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
4081 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc
4141 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
4201 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct
4261 tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat
4321 tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg
4381 cagaagagct ggggagaatt aattcgtaat catggtcata gctgtttcct gtgtgaaatt
4441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
4501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
4561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
4621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
4681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
4741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
4801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
4861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
4921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
4981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
5041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
5101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
5161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
5221 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
5281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
5341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
5401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
5461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
5521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
5581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
5641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
5701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
5761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
5821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
5881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
5941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
6001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
6061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
6121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
6181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
6241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
6301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
6361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
6421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
6481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
6541 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
6601 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
6661 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
6721 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
6781 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
6841 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
6901 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
6961 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
7021 cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
7081 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
7141 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
7201 gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc
7261 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga
7321 gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
//