LOCUS LT726943 8432 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCD-F-HLH-MK-E4A-bio, complete
sequence.
ACCESSION LT726943
VERSION LT726943.1
KEYWORDS .
SOURCE Mammalian expression vector pCD-F-HLH-MK-E4A-bio
ORGANISM Mammalian expression vector pCD-F-HLH-MK-E4A-bio
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8432)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8432
/organism="Mammalian expression vector
pCD-F-HLH-MK-E4A-bio"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5683."
/db_xref="taxon:1944908"
regulatory 235..818
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory 863..879
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 923..946
/note="FLAG epitope"
CDS 968..>1447
/transl_table=11
/note="HLH dimerization domain"
/protein_id="SJL87026.1"
/translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSYA
SSTPLHVPVPRALRMEEDSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNT
FEMNGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKPRILFSPFFHPGNSIHTQP
"
misc_feature 971..973
/note="Kozak"
CDS 1463..>3934
/transl_table=11
/note="hMLT mutant"
/protein_id="SJL87027.1"
/translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSEL
LDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCT
VTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQW
FKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRS
VDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYM
VPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDAAVACTEDELNNLGHPDNKEQT
TDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMR
NAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQ
EKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLAN
GIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQG
TEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPE
IIMCDAYVTDFPLDLDIDPKDANKGTPEATGSYLVSKDLPKHCLYTRLSSLQKLKEHL
VFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSS
AATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHF
SRSNVPVATTDEIPFSFSDRLRISEK"
misc_feature 1466..1468
/note="Kozak"
misc_feature 2399..2401
/note="E313A"
misc_feature 2408..2410
/note="E316A"
misc_feature 2948..2950
/note="stability creator"
misc_feature 3410..3412
/note="PCR artefact"
misc_feature 3419..3427
/note="E653A"
misc_feature 3875..3880
/note="E806A"
misc_feature 3950..3994
/note="bio-tag"
regulatory 4029..4253
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 4299..4727
/note="f1 ori"
regulatory 4732..5013
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4970..5045
/note="SV40 ori"
CDS 5137..5931
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87028.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 6105..6179
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(6105..6179)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(6318..6401)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(6493..6895)
/note="pMB1 ori"
CDS complement(7436..8296)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87029.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2079 a 2087 c 2110 g 2156 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc
961 tgccaccatg gcgctgttgg gggacccgct acaggccctg ccgccctcgg ccgcccccac
1021 ggggccgctg ctcgcccctc cggccggcgc gaccctcaac cgcctgcggg agccgctgct
1081 gcggaggctc agctacgctt cctcgacgcc acttcatgtt ccagtgcctc gagcgctcag
1141 gatggaggaa gactcgatcc gcctgcctgc gcacctgcgc ttgcagccaa tttactggag
1201 cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt taaggccaat
1261 tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca aagaggactt
1321 tcgctatcga tctcctcatt caggtgatgt gctctatgaa ctccttcagc atattctgaa
1381 gcagaggaaa cctcggattc ttttttcacc attcttccac cctggaaact ctatacacac
1441 acagccggtt gatcctgcca ccatggcgct gttgggggac ccgctacagg ccctgccgcc
1501 ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc tcaaccgcct
1561 gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg agggccgggg
1621 ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca gttgcctaga
1681 cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc tgtgtctgct
1741 gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc tgcaggctat
1801 ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta ctgtaaaccc
1861 agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg caactggaca
1921 tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg gaaatacatc
1981 agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct gtcgagttaa
2041 taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg acatcccaga
2101 gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct gtgttgaacc
2161 aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg ctgttggaag
2221 ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg agaccaaaaa
2281 gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt gtcatgtata
2341 taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa gaacagatgc
2401 ggcagtggca tgcactgaag atgaattaaa taatcttggt catcctgata ataaagagca
2461 aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa atatgaatta
2521 ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga ctaacttact
2581 gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg agatgcgtaa
2641 tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat tatattatgc
2701 aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg ctccaaatcc
2761 atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc aagaaaaaga
2821 aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact acgatgatac
2881 cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat atgccacgtg
2941 tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa tctttatgaa
3001 atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg atgaagttgc
3061 agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga ttcgaagtag
3121 tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt ctgctgaatc
3181 tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta tgtgtcttaa
3241 gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca atgtcatgat
3301 catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg cctacgttac
3361 tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca cgcctgaagc
3421 aaccggtagc tacttggtat caaaggatct tcccaagcat tgcctctata ccagactcag
3481 ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat atcagtactc
3541 aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac ctctcattgc
3601 taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt gtcttatgtc
3661 taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt atcactcatt
3721 gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta atgttacacc
3781 agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt tcgctcacca
3841 tgcttcatgt cattttagta gaagtaatgt gccagtcgcg acaactgatg aaataccatt
3901 tagtttctct gacaggctca gaatttctga aaaagggccc gtttccggag gcctgaacga
3961 catcttcgag gcccagaaga tcgagtggca cgagtaaggg cccgtttaaa cccgctgatc
4021 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
4081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
4141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
4201 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
4261 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
4321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
4381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
4441 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
4501 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
4561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
4621 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
4681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
4741 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
4801 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
4861 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
4921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
4981 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
5041 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
5101 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
5161 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
5221 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
5281 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
5341 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
5401 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
5461 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
5521 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
5581 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
5641 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
5701 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
5761 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
5821 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
5881 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
5941 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
6001 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
6061 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
6121 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
6181 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
6241 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
6301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
6361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
6421 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
6481 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
6541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
6601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
6661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
6721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
6781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
6841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
6901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
6961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
7021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
7081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
7141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
7201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
7261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
7321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
7381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
7441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
7501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
7561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
7621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
7681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
7741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
7801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
7861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
7921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
7981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
8041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
8101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
8161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
8221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
8281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
8341 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
8401 gcacatttcc ccgaaaagtg ccacctgacg tc
//