LOCUS LT726942 8447 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCD-F-HLH-MK-bioC, complete sequence. ACCESSION LT726942 VERSION LT726942.1 KEYWORDS . SOURCE Mammalian expression vector pCD-F-HLH-MK-bioC ORGANISM Mammalian expression vector pCD-F-HLH-MK-bioC other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8447) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..8447 /organism="Mammalian expression vector pCD-F-HLH-MK-bioC" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5681." /db_xref="taxon:1944907" regulatory 235..818 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 863..879 /note="T7 promoter" /regulatory_class="promoter" misc_feature 923..946 /note="FLAG epitope" CDS 968..>1447 /transl_table=11 /note="HLH dimerization domain" /protein_id="SJL87022.1" /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSYA SSTPLHVPVPRALRMEEDSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNT FEMNGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKPRILFSPFFHPGNSIHTQP " misc_feature 971..973 /note="Kozak" CDS 1463..>3934 /transl_table=11 /note="hMLT" /protein_id="SJL87023.1" /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSEL LDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCT VTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQW FKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRS VDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYM VPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDEAVECTEDELNNLGHPDNKEQT TDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMR NAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQ EKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLAN GIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQG TEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPE IIMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHL VFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSS AATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHF SRSNVPVETTDEIPFSFSDRLRISEK" misc_feature 1466..1468 /note="Kozak" misc_feature 2948..2950 /note="stability creator" misc_feature 3410..3412 /note="PCR artefact" misc_feature 3941..3964 /note="SGSSGSSG linker" misc_feature 3965..4009 /note="bio-tag" regulatory 4044..4268 /note="BGH polyA" /regulatory_class="terminator" rep_origin 4314..4742 /note="f1 ori" regulatory 4747..5028 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 4985..5060 /note="SV40 ori" CDS 5152..5946 /transl_table=11 /note="Tn5 neomycin resistance" /protein_id="SJL87024.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory complement(6120..6194) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 6120..6194 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(6333..6416) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(6508..6910) /note="pMB1 ori" CDS complement(7451..8311) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87025.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2088 a 2085 c 2116 g 2158 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 961 tgccaccatg gcgctgttgg gggacccgct acaggccctg ccgccctcgg ccgcccccac 1021 ggggccgctg ctcgcccctc cggccggcgc gaccctcaac cgcctgcggg agccgctgct 1081 gcggaggctc agctacgctt cctcgacgcc acttcatgtt ccagtgcctc gagcgctcag 1141 gatggaggaa gactcgatcc gcctgcctgc gcacctgcgc ttgcagccaa tttactggag 1201 cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt taaggccaat 1261 tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca aagaggactt 1321 tcgctatcga tctcctcatt caggtgatgt gctctatgaa ctccttcagc atattctgaa 1381 gcagaggaaa cctcggattc ttttttcacc attcttccac cctggaaact ctatacacac 1441 acagccggtt gatcctgcca ccatggcgct gttgggggac ccgctacagg ccctgccgcc 1501 ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc tcaaccgcct 1561 gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg agggccgggg 1621 ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca gttgcctaga 1681 cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc tgtgtctgct 1741 gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc tgcaggctat 1801 ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta ctgtaaaccc 1861 agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg caactggaca 1921 tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg gaaatacatc 1981 agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct gtcgagttaa 2041 taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg acatcccaga 2101 gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct gtgttgaacc 2161 aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg ctgttggaag 2221 ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg agaccaaaaa 2281 gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt gtcatgtata 2341 taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa gaacagatga 2401 ggcagtggag tgcactgaag atgaattaaa taatcttggt catcctgata ataaagagca 2461 aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa atatgaatta 2521 ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga ctaacttact 2581 gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg agatgcgtaa 2641 tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat tatattatgc 2701 aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg ctccaaatcc 2761 atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc aagaaaaaga 2821 aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact acgatgatac 2881 cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat atgccacgtg 2941 tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa tctttatgaa 3001 atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg atgaagttgc 3061 agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga ttcgaagtag 3121 tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt ctgctgaatc 3181 tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta tgtgtcttaa 3241 gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca atgtcatgat 3301 catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg cctacgttac 3361 tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca cgcctgaaga 3421 aactggcagc tacttggtat caaaggatct tcccaagcat tgcctctata ccagactcag 3481 ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat atcagtactc 3541 aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac ctctcattgc 3601 taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt gtcttatgtc 3661 taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt atcactcatt 3721 gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta atgttacacc 3781 agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt tcgctcacca 3841 tgcttcatgt cattttagta gaagtaatgt gccagtagag acaactgatg aaataccatt 3901 tagtttctct gacaggctca gaatttctga aaaagggccc agcggaagtt caggttcatc 3961 cggaggcctg aacgacatct tcgaggccca gaagatcgag tggcacgagt aagggcccgt 4021 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 4081 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 4141 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 4201 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 4261 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 4321 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 4381 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 4441 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 4501 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 4561 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 4621 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 4681 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 4741 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 4801 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 4861 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 4921 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 4981 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 5041 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 5101 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 5161 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 5221 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 5281 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 5341 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 5401 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 5461 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 5521 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 5581 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 5641 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 5701 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 5761 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 5821 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 5881 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 5941 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 6001 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 6061 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 6121 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6181 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6241 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 6301 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 6361 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 6421 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 6481 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 6541 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6601 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 7021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 7081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 7141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 7201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 7261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 7321 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 7381 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 7441 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7501 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7561 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7621 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7681 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7741 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7801 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7861 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7921 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7981 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 8041 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 8101 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 8161 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 8221 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 8281 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 8341 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 8401 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //