LOCUS LT726941 7919 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCD-F-bio-MK-E4A, complete sequence. ACCESSION LT726941 VERSION LT726941.1 KEYWORDS . SOURCE Mammalian expression vector pCD-F-bio-MK-E4A ORGANISM Mammalian expression vector pCD-F-bio-MK-E4A other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7919) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7919 /organism="Mammalian expression vector pCD-F-bio-MK-E4A" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5680." /db_xref="taxon:1944906" regulatory 235..818 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 863..879 /note="T7 promoter" /regulatory_class="promoter" misc_feature 923..946 /note="FLAG epitope" misc_feature 953..997 /note="bio-tag" CDS 1013..>3484 /transl_table=11 /note="hMLT mutant" /protein_id="SJL87019.1" /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSEL LDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCT VTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQW FKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRS VDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYM VPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDAAVACTEDELNNLGHPDNKEQT TDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMR NAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQ EKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLAN GIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQG TEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPE IIMCDAYVTDFPLDLDIDPKDANKGTPEATGSYLVSKDLPKHCLYTRLSSLQKLKEHL VFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSS AATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHF SRSNVPVATTDEIPFSFSDRLRISEK" misc_feature 1016..1018 /note="Kozak" misc_feature 1949..1951 /note="E313A" misc_feature 1958..1960 /note="E316A" misc_feature 2498..2500 /note="stability creator" misc_feature 2960..2962 /note="PCR artefact" misc_feature 2969..2977 /note="E653A" misc_feature 3425..3430 /note="E806A" regulatory 3516..3740 /note="BGH polyA" /regulatory_class="terminator" rep_origin 3786..4214 /note="f1 ori" regulatory 4219..4500 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 4457..4532 /note="SV40 ori" CDS 4624..5418 /transl_table=11 /note="Tn5 neomycin resistance" /protein_id="SJL87020.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 5592..5666 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(5592..5666) /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(5805..5888) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(5980..6382) /note="pMB1 ori" CDS complement(6923..7783) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87021.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1978 a 1921 c 1978 g 2042 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caggcctgaa 961 cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctgcca ccatggcgct 1021 gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc 1081 ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga 1141 gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc tggcggggag 1201 tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta aggtactgga 1261 gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt 1321 cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc agcttctcag 1381 ccccccagga ataaagatta ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt 1441 tgtgaaactg tgttgccggg caactggaca tccttttgtt caatatcagt ggttcaaaat 1501 gaataaagag attccaaatg gaaatacatc agagcttatt tttaatgcag tgcatgtaaa 1561 agatgcaggc ttttatgtct gtcgagttaa taacaatttc acctttgaat tcagccagtg 1621 gtcacagctg gatgtttgcg acatcccaga gagcttccag agaagtgttg atggcgtctc 1681 tgaatccaag ttgcaaatct gtgttgaacc aacttcccaa aagctgatgc caggcagcac 1741 attggtttta cagtgtgttg ctgttggaag ccctattcct cactaccagt ggttcaaaaa 1801 tgaattacca ttaacacatg agaccaaaaa gctatacatg gtgccttatg tggatttgga 1861 acaccaagga acctactggt gtcatgtata taatgatcga gacagtcaag atagcaagaa 1921 ggtagaaatc atcataggaa gaacagatgc ggcagtggca tgcactgaag atgaattaaa 1981 taatcttggt catcctgata ataaagagca aacaactgac cagcctttgg cgaaggacaa 2041 ggttgccctt ttgataggaa atatgaatta ccgggagcac cccaagctca aagctccttt 2101 ggtggatgtg tacgaattga ctaacttact gagacagctg gacttcaaag tggtttcact 2161 gttggatctt actgaatatg agatgcgtaa tgctgtggat gagtttttac tccttttaga 2221 caagggagta tatgggttat tatattatgc aggacatggt tatgaaaatt ttgggaacag 2281 cttcatggtc cccgttgatg ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca 2341 aaatatactg aaattgatgc aagaaaaaga aactggactt aatgtgttct tattggatat 2401 gtgtaggaaa agaaatgact acgatgatac cattccaatc ttggatgcac taaaagtcac 2461 cgccaatatt gtgtttggat atgccacgtg tcaaggagct gaagcttttg aaatccagca 2521 ttctggattg gcaaatggaa tctttatgaa atttttaaaa gacagattat tagaagataa 2581 gaaaatcact gtgttactgg atgaagttgc agaagatatg ggtaagtgtc accttaccaa 2641 aggcaaacag gctctagaga ttcgaagtag tttatctgag aagagagcac ttactgatcc 2701 aatacaggga acagaatatt ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc 2761 tcatgaactt ccagaaagta tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg 2821 atttgcagct gagttttcca atgtcatgat catctataca agtatagttt acaaaccacc 2881 ggagataata atgtgtgatg cctacgttac tgattttcca cttgatctag atattgatcc 2941 aaaagatgca aataaaggca cgcctgaagc aaccggtagc tacttggtat caaaggatct 3001 tcccaagcat tgcctctata ccagactcag ttcactgcaa aaattaaagg aacatctagt 3061 cttcacagta tgtttatcat atcagtactc aggattggaa gatactgtag aggacaagca 3121 ggaagtgaat gttgggaaac ctctcattgc taaattagac atgcatcgag gtttgggaag 3181 gaagacttgc tttcaaactt gtcttatgtc taatggtcct taccagagtt ctgcagccac 3241 ctcaggagga gcagggcatt atcactcatt gcaagaccca ttccatggtg tttaccattc 3301 acatcctggt aatccaagta atgttacacc agcagatagc tgtcattgca gccggactcc 3361 agatgcattt atttcaagtt tcgctcacca tgcttcatgt cattttagta gaagtaatgt 3421 gccagtcgcg acaactgatg aaataccatt tagtttctct gacaggctca gaatttctga 3481 aaaagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 3541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 3601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 3661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 3721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 3781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 3841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 3901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 3961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 4021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 4081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 4141 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 4201 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 4261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 4321 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 4381 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 4441 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 4501 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 4561 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 4621 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 4681 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 4741 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 4801 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 4861 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 4921 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 4981 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 5041 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 5101 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 5161 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 5221 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 5281 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 5341 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 5401 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 5461 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 5521 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 5581 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 5641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 5701 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 5761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 5821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 5881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 5941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 6001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 6061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 6121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 6181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 6241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 6301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 6361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 6421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 6481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 6601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 6661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 6721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 6781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 6841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 6901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 7021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 7081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 7141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 7201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 7261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 7321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 7381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 7441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 7501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 7561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 7621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 7681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 7741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 7801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 7861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc //