LOCUS LT726940 7919 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCD-F-bio-MK, complete sequence.
ACCESSION LT726940
VERSION LT726940.1
KEYWORDS .
SOURCE Mammalian expression vector pCD-F-bio-MK
ORGANISM Mammalian expression vector pCD-F-bio-MK
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7919)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7919
/organism="Mammalian expression vector pCD-F-bio-MK"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5679."
/db_xref="taxon:1944905"
regulatory 235..818
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory 863..879
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 923..946
/note="FLAG epitope"
misc_feature 953..997
/note="bio-tag"
CDS 1013..>3484
/transl_table=11
/note="hMLT"
/protein_id="SJL87016.1"
/translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSEL
LDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCT
VTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQW
FKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRS
VDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYM
VPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDEAVECTEDELNNLGHPDNKEQT
TDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMR
NAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQ
EKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLAN
GIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQG
TEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPE
IIMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHL
VFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSS
AATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHF
SRSNVPVETTDEIPFSFSDRLRISEK"
misc_feature 1016..1018
/note="Kozak"
misc_feature 2498..2500
/note="stability creator"
misc_feature 2960..2962
/note="PCR artefact"
regulatory 3516..3740
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 3786..4214
/note="f1 ori"
regulatory 4219..4500
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4457..4532
/note="SV40 ori"
CDS 4624..5418
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87017.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5592..5666
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5592..5666)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(5805..5888)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5980..6382)
/note="pMB1 ori"
CDS complement(6923..7783)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87018.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1982 a 1916 c 1979 g 2042 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caggcctgaa
961 cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctgcca ccatggcgct
1021 gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc
1081 ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga
1141 gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc tggcggggag
1201 tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta aggtactgga
1261 gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt
1321 cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc agcttctcag
1381 ccccccagga ataaagatta ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt
1441 tgtgaaactg tgttgccggg caactggaca tccttttgtt caatatcagt ggttcaaaat
1501 gaataaagag attccaaatg gaaatacatc agagcttatt tttaatgcag tgcatgtaaa
1561 agatgcaggc ttttatgtct gtcgagttaa taacaatttc acctttgaat tcagccagtg
1621 gtcacagctg gatgtttgcg acatcccaga gagcttccag agaagtgttg atggcgtctc
1681 tgaatccaag ttgcaaatct gtgttgaacc aacttcccaa aagctgatgc caggcagcac
1741 attggtttta cagtgtgttg ctgttggaag ccctattcct cactaccagt ggttcaaaaa
1801 tgaattacca ttaacacatg agaccaaaaa gctatacatg gtgccttatg tggatttgga
1861 acaccaagga acctactggt gtcatgtata taatgatcga gacagtcaag atagcaagaa
1921 ggtagaaatc atcataggaa gaacagatga ggcagtggag tgcactgaag atgaattaaa
1981 taatcttggt catcctgata ataaagagca aacaactgac cagcctttgg cgaaggacaa
2041 ggttgccctt ttgataggaa atatgaatta ccgggagcac cccaagctca aagctccttt
2101 ggtggatgtg tacgaattga ctaacttact gagacagctg gacttcaaag tggtttcact
2161 gttggatctt actgaatatg agatgcgtaa tgctgtggat gagtttttac tccttttaga
2221 caagggagta tatgggttat tatattatgc aggacatggt tatgaaaatt ttgggaacag
2281 cttcatggtc cccgttgatg ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca
2341 aaatatactg aaattgatgc aagaaaaaga aactggactt aatgtgttct tattggatat
2401 gtgtaggaaa agaaatgact acgatgatac cattccaatc ttggatgcac taaaagtcac
2461 cgccaatatt gtgtttggat atgccacgtg tcaaggagct gaagcttttg aaatccagca
2521 ttctggattg gcaaatggaa tctttatgaa atttttaaaa gacagattat tagaagataa
2581 gaaaatcact gtgttactgg atgaagttgc agaagatatg ggtaagtgtc accttaccaa
2641 aggcaaacag gctctagaga ttcgaagtag tttatctgag aagagagcac ttactgatcc
2701 aatacaggga acagaatatt ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc
2761 tcatgaactt ccagaaagta tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg
2821 atttgcagct gagttttcca atgtcatgat catctataca agtatagttt acaaaccacc
2881 ggagataata atgtgtgatg cctacgttac tgattttcca cttgatctag atattgatcc
2941 aaaagatgca aataaaggca cgcctgaaga aactggcagc tacttggtat caaaggatct
3001 tcccaagcat tgcctctata ccagactcag ttcactgcaa aaattaaagg aacatctagt
3061 cttcacagta tgtttatcat atcagtactc aggattggaa gatactgtag aggacaagca
3121 ggaagtgaat gttgggaaac ctctcattgc taaattagac atgcatcgag gtttgggaag
3181 gaagacttgc tttcaaactt gtcttatgtc taatggtcct taccagagtt ctgcagccac
3241 ctcaggagga gcagggcatt atcactcatt gcaagaccca ttccatggtg tttaccattc
3301 acatcctggt aatccaagta atgttacacc agcagatagc tgtcattgca gccggactcc
3361 agatgcattt atttcaagtt tcgctcacca tgcttcatgt cattttagta gaagtaatgt
3421 gccagtagag acaactgatg aaataccatt tagtttctct gacaggctca gaatttctga
3481 aaaagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
3541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
3601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
3661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
3721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
3781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
3841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
3901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
3961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
4021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
4141 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
4201 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
4261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
4321 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
4381 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
4441 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
4501 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
4561 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
4621 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
4681 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
4741 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
4801 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
4861 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
4921 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
4981 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
5041 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
5101 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
5161 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
5221 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
5281 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
5341 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
5401 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
5461 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
5521 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
5581 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
5641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
5701 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
5761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
5821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
5881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
5941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
6001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
6061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
6121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
6181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
6241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
6301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
6361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
6421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
6481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
6541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
6601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
6661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
6721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
6781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
6841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
6901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
6961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
7021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
7081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
7141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
7201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
7261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
7321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
7381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
7441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
7501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
7561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
7621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
7681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
7741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
7801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
7861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//