LOCUS LT726926 11283 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pAS2.1-MLT3b, complete sequence.
ACCESSION LT726926
VERSION LT726926.1
KEYWORDS .
SOURCE Yeast expression vector pAS2.1-MLT3b
ORGANISM Yeast expression vector pAS2.1-MLT3b
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 11283)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..11283
/organism="Yeast expression vector pAS2.1-MLT3b"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5542."
/db_xref="taxon:1945446"
rep_origin complement(2..982)
/note="2mu ori"
regulatory complement(1060..1164)
/note="FLP terminator"
/regulatory_class="terminator"
CDS 1884..2558
/transl_table=11
/note="TRP1"
/protein_id="SJL86968.1"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK"
rep_origin complement(2661..3116)
/note="f1 ori"
regulatory complement(3312..3586)
/note="CYC1 terminator"
/regulatory_class="terminator"
CDS complement(3855..4304)
/transl_table=11
/note="CYH2"
/protein_id="SJL86969.1"
/translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN
MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI
DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA"
regulatory 5075..5478
/note="ADH1 promoter"
/regulatory_class="promoter"
CDS 5501..>5941
/transl_table=11
/note="GALbd"
/protein_id="SJL86970.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
CDS <5993..>7555
/codon_start=1
/transl_table=11
/note="hMLT fragment"
/protein_id="SJL86971.1"
/translation="VEIIIGRTDEAVECTEDELNNLGHPDNKEQTTDQPLAKDKVALL
IGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKG
VYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDM
CRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLANGIFMKFLKDRLLE
DKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQGTEYSAESLVRNLQ
WAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPEIIMCDAYVTDFPL
DLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHLVFTVCLSYQYSGL
EDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSSAATSGGAGHYHSL
QDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHFSRSNVPVETTDEI
PFSFSDRLRISEK"
misc_feature 6569..6571
/note="stability creator"
misc_feature 7031..7033
/note="PCR artefact"
regulatory 7583..7684
/note="ADH1 terminator"
/regulatory_class="terminator"
CDS complement(7751..8959)
/transl_table=11
/note="IS10R"
/protein_id="SJL86972.1"
/translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIR
TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNL
FTHGYALGKL"
misc_feature complement(9137..9174)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(9175..9258)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(9350..9752)
/note="pMB1 ori"
CDS complement(10293..11153)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86973.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 3187 a 2299 c 2526 g 3271 t
ORIGIN
1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc
3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc
5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
5941 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcgtagaaat
6001 catcatagga agaacagatg aggcagtgga gtgcactgaa gatgaattaa ataatcttgg
6061 tcatcctgat aataaagagc aaacaactga ccagcctttg gcgaaggaca aggttgccct
6121 tttgatagga aatatgaatt accgggagca ccccaagctc aaagctcctt tggtggatgt
6181 gtacgaattg actaacttac tgagacagct ggacttcaaa gtggtttcac tgttggatct
6241 tactgaatat gagatgcgta atgctgtgga tgagttttta ctccttttag acaagggagt
6301 atatgggtta ttatattatg caggacatgg ttatgaaaat tttgggaaca gcttcatggt
6361 ccccgttgat gctccaaatc catataggtc tgaaaattgt ctgtgtgtac aaaatatact
6421 gaaattgatg caagaaaaag aaactggact taatgtgttc ttattggata tgtgtaggaa
6481 aagaaatgac tacgatgata ccattccaat cttggatgca ctaaaagtca ccgccaatat
6541 tgtgtttgga tatgccacgt gtcaaggagc tgaagctttt gaaatccagc attctggatt
6601 ggcaaatgga atctttatga aatttttaaa agacagatta ttagaagata agaaaatcac
6661 tgtgttactg gatgaagttg cagaagatat gggtaagtgt caccttacca aaggcaaaca
6721 ggctctagag attcgaagta gtttatctga gaagagagca cttactgatc caatacaggg
6781 aacagaatat tctgctgaat ctcttgtgcg gaatctacag tgggccaagg ctcatgaact
6841 tccagaaagt atgtgtctta agtttgactg tggtgttcag attcaattag gatttgcagc
6901 tgagttttcc aatgtcatga tcatctatac aagtatagtt tacaaaccac cggagataat
6961 aatgtgtgat gcctacgtta ctgattttcc acttgatcta gatattgatc caaaagatgc
7021 aaataaaggc acgcctgaag aaactggcag ctacttggta tcaaaggatc ttcccaagca
7081 ttgcctctat accagactca gttcactgca aaaattaaag gaacatctag tcttcacagt
7141 atgtttatca tatcagtact caggattgga agatactgta gaggacaagc aggaagtgaa
7201 tgttgggaaa cctctcattg ctaaattaga catgcatcga ggtttgggaa ggaagacttg
7261 ctttcaaact tgtcttatgt ctaatggtcc ttaccagagt tctgcagcca cctcaggagg
7321 agcagggcat tatcactcat tgcaagaccc attccatggt gtttaccatt cacatcctgg
7381 taatccaagt aatgttacac cagcagatag ctgtcattgc agccggactc cagatgcatt
7441 tatttcaagt ttcgctcacc atgcttcatg tcattttagt agaagtaatg tgccagtaga
7501 gacaactgat gaaataccat ttagtttctc tgacaggctc agaatttctg aaaaagtcga
7561 cctgcagcca agctaattcc gggcgaattt cttatgattt atgattttta ttattaaata
7621 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
7681 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcactg
7741 agagatcccc tcataatttc cccaaagcgt aaccatgtgt gaataaattt tgagctagta
7801 gggttgcagc cacgagtaag tcttcccttg ttattgtgta gccagaatgc cgcaaaactt
7861 ccatgcctaa gcgaactgtt gagagtacgt ttcgatttct gactgtgtta gcctggaagt
7921 gcttgtccca accttgtttc tgagcatgaa cgcccgcaag ccaacatgtt agttgaagca
7981 tcagggcgat tagcagcatg atatcaaaac gctctgagct gctcgttcgg ctatggcgta
8041 ggcctagtcc gtaggcagga cttttcaagt ctcggaaggt ttcttcaatc tgcattcgct
8101 tcgaatagat attaacaagt tgtttgggtg ttcgaatttc aacaggtaag ttagttgcta
8161 gaacccatgg ctcctttgcc gacgctgagt agattttagg tgacgggtgg tgacaatgag
8221 tccgtgtcga gcgctgattt tttcggcctt tagagcgaga tttatacaat agaatttggc
8281 atgagattgg attgctttta gtcagcctct tatagcctaa agtctttgag tgactagatg
8341 acatatcatg taagttgctg ataggtttcc agttttccgc tcctaggtct gcatattgta
8401 cttttcctct tactcgactt aaccagtacc aacccagctt ctcaacggat ttataccatg
8461 gcactttaaa gccagcatca ctgacaatga gcggtgtggt gttactcggt agaatgctcg
8521 caaggtcggc tagaaattgg tcatgagctt tctttgaaca ttgctctgaa agcgggaacg
8581 ctttctcata aagagtaaca gaacgaccgt gtagtgcgac tgaagctcgc aataccataa
8641 gtcgtttttg ctcacgaata tcagaccagt caacaagtac aatgggcatc gtattgcccg
8701 aacagataaa gctagcatgc caacggtata cagcgagtcg ctctttgtgg aggtgacgat
8761 tacctaacaa tcggtcgatt cgtttgatgt tatgttttgt tctcgctttg gttggcaggt
8821 tacggccaag ttcggtaaga gtgagagttt tacagtcaag taatgcgtgg caagccaacg
8881 ttaagctgtt gagtcgtttt aagtgtaatt cggggcagaa ttggtaaaga gagtcgtgta
8941 aaatatcgag ttcgcacatc ttgttgtctg attattgatt tttcgcgaaa ccatttgatc
9001 atatgacaag atgtgtatcc accttaactt aatgattttt accaaaatca ttaggggatt
9061 catcagggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg
9121 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
9181 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc
9241 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
9301 gattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
9361 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
9421 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
9481 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
9541 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
9601 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
9661 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
9721 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
9781 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
9841 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tagtaacagg
9901 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
9961 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
10021 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
10081 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
10141 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
10201 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
10261 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
10321 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
10381 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
10441 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
10501 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
10561 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
10621 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
10681 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
10741 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
10801 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
10861 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
10921 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
10981 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
11041 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
11101 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
11161 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
11221 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
11281 cct
//