LOCUS LT726924 10338 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pAS2.1-MLT3a, complete sequence.
ACCESSION LT726924
VERSION LT726924.1
KEYWORDS .
SOURCE Yeast expression vector pAS2.1-MLT3a
ORGANISM Yeast expression vector pAS2.1-MLT3a
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 10338)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..10338
/organism="Yeast expression vector pAS2.1-MLT3a"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5540."
/db_xref="taxon:1945445"
rep_origin complement(2..982)
/note="2mu ori"
regulatory complement(1060..1164)
/note="FLP terminator"
/regulatory_class="terminator"
CDS 1884..2558
/transl_table=11
/note="TRP1"
/protein_id="SJL86959.1"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK"
rep_origin complement(2661..3116)
/note="f1 ori"
regulatory complement(3312..3586)
/note="CYC1 terminator"
/regulatory_class="terminator"
CDS complement(3855..4304)
/transl_table=11
/note="CYH2"
/protein_id="SJL86960.1"
/translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN
MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI
DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA"
regulatory 5075..5478
/note="ADH1 promoter"
/regulatory_class="promoter"
CDS 5501..>5941
/transl_table=11
/note="GALbd"
/protein_id="SJL86961.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
CDS <5993..>6610
/codon_start=1
/transl_table=11
/note="hMLT fragment"
/protein_id="SJL86962.1"
/translation="VEIIIGRTDEAVECTEDELNNLGHPDNKEQTTDQPLAKDKVALL
IGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKG
VYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDM
CRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLANG"
misc_feature 6569..6571
/note="stability creator"
regulatory 6638..6739
/note="ADH1 terminator"
/regulatory_class="terminator"
CDS complement(6806..8014)
/transl_table=11
/note="IS10R"
/protein_id="SJL86963.1"
/translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIR
TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNL
FTHGYALGKL"
misc_feature complement(8192..8229)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(8230..8313)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(8405..8807)
/note="pMB1 ori"
CDS complement(9348..10208)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86964.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2891 a 2119 c 2330 g 2998 t
ORIGIN
1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc
3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc
5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
5941 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcgtagaaat
6001 catcatagga agaacagatg aggcagtgga gtgcactgaa gatgaattaa ataatcttgg
6061 tcatcctgat aataaagagc aaacaactga ccagcctttg gcgaaggaca aggttgccct
6121 tttgatagga aatatgaatt accgggagca ccccaagctc aaagctcctt tggtggatgt
6181 gtacgaattg actaacttac tgagacagct ggacttcaaa gtggtttcac tgttggatct
6241 tactgaatat gagatgcgta atgctgtgga tgagttttta ctccttttag acaagggagt
6301 atatgggtta ttatattatg caggacatgg ttatgaaaat tttgggaaca gcttcatggt
6361 ccccgttgat gctccaaatc catataggtc tgaaaattgt ctgtgtgtac aaaatatact
6421 gaaattgatg caagaaaaag aaactggact taatgtgttc ttattggata tgtgtaggaa
6481 aagaaatgac tacgatgata ccattccaat cttggatgca ctaaaagtca ccgccaatat
6541 tgtgtttgga tatgccacgt gtcaaggagc tgaagctttt gaaatccagc attctggatt
6601 ggcaaatgga gtcgacctgc agccaagcta attccgggcg aatttcttat gatttatgat
6661 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
6721 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
6781 tcaggtatag cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
6841 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
6901 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
6961 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
7021 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
7081 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
7141 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
7201 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
7261 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
7321 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
7381 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
7441 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
7501 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
7561 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
7621 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
7681 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
7741 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
7801 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
7861 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
7921 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg cagaattggt
7981 aaagagagtc gtgtaaaata tcgagttcgc acatcttgtt gtctgattat tgatttttcg
8041 cgaaaccatt tgatcatatg acaagatgtg tatccacctt aacttaatga tttttaccaa
8101 aatcattagg ggattcatca gggtatagca tgaggtcgct cttattgacc acacctctac
8161 cggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
8221 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
8281 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
8341 ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
8401 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
8461 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
8521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
8581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
8641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
8701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
8761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
8821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
8881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
8941 gccactagta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
9001 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
9061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
9121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
9181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
9241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
9301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
9361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
9421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
9481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
9541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
9601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
9661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
9721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
9781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
9841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
9901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
9961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
10021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
10081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
10141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
10201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
10261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
10321 ccccgaaaag tgccacct
//