LOCUS LT726921 8276 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCD-A7M8K, complete sequence.
ACCESSION LT726921
VERSION LT726921.1
KEYWORDS .
SOURCE Mammalian expression vector pCD-A7M8K
ORGANISM Mammalian expression vector pCD-A7M8K
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8276)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8276
/organism="Mammalian expression vector pCD-A7M8K"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5537."
/db_xref="taxon:1944902"
regulatory 235..818
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory 863..879
/note="T7 promoter"
/regulatory_class="promoter"
CDS 933..>2256
/transl_table=11
/note="hAPI2 fragment"
/protein_id="SJL86945.1"
/translation="MDIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVP
VSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSV
NNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRS
SYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPK
DNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQL
ASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQV
QASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSR
SLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKES"
misc_feature 936..938
/note="Kozak"
misc_feature 2184..2186
/note="PCR artefact"
CDS <2257..>3770
/codon_start=3
/transl_table=11
/note="hMLT fragment"
/protein_id="SJL86946.1"
/translation="ELNNLGHPDNKEQTTDQPLAKDKVALLIGNMNYREHPKLKAPLV
DVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGN
SFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDMCRKRNDYDDTIPILDAL
KVTANIVFGYATCQGAEAFEIQHSGLANGIFMKFLKDRLLEDKKITVLLDEVAEDMGK
CHLTKGKQALEIRSSLSEKRALTDPIQGTEYSAESLVRNLQWAKAHELPESMCLKFDC
GVQIQLGFAAEFSNVMIIYTSIVYKPPEIIMCDAYVTDFPLDLDIDPKDANKGTPEET
GSYLVSKDLPKHCLYTRLSSLQKLKEHLVFTVCLSYQYSGLEDTVEDKQEVNVGKPLI
AKLDMHRGLGRKTCFQTCLMSNGPYQSSAATSGGAGHYHSLQDPFHGVYHSHPGNPSN
VTPADSCHCSRTPDAFISSFAHHASCHFSRSNVPVETTDEIPFSFSDRLRISEK"
misc_feature 2784..2786
/note="stability creator"
misc_feature 3246..3248
/note="PCR artefact"
misc_feature 3780..3809
/note="Myc epitope"
misc_feature 3825..3842
/note="His-tag"
regulatory 3871..4095
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 4141..4569
/note="f1 ori"
regulatory 4574..4855
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4812..4887
/note="SV40 ori"
CDS 4979..5773
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL86947.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory complement(5949..6023)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 5949..6023
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(6162..6245)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(6337..6739)
/note="pMB1 ori"
CDS complement(7280..8140)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86948.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2141 a 1955 c 1994 g 2186 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagcttggt accgagctcg gatcctgcca ccatggacat agtagaaaac agcatattct
961 tatcaaattt gatgaaaagc gccaacacgt ttgaactgaa atacgacttg tcatgtgaac
1021 tgtaccgaat gtctacgtat tccacttttc ctgctggggt tcctgtctca gaaaggagtc
1081 ttgctcgtgc tggtttctat tacactggtg tgaatgacaa ggtcaaatgc ttctgttgtg
1141 gcctgatgct ggataactgg aaaagaggag acagtcctac tgaaaagcat aaaaagttgt
1201 atcctagctg cagattcgtt cagagtctaa attccgttaa caacttggaa gctacctctc
1261 agcctacttt tccttcttca gtaacaaatt ccacacactc attacttccg ggtacagaaa
1321 acagtggata tttccgtggc tcttattcaa actctccatc aaatcctgta aactccagag
1381 caaatcaaga tttttctgcc ttgatgagaa gttcctacca ctgtgcaatg aataacgaaa
1441 atgccagatt acttactttt cagacatggc cattgacttt tctgtcgcca acagatctgg
1501 caaaagcagg cttttactac ataggacctg gagacagagt ggcttgcttt gcctgtggtg
1561 gaaaattgag caattgggaa ccgaaggata atgctatgtc agaacacctg agacattttc
1621 ccaaatgccc atttatagaa aatcagcttc aagacacttc aagatacaca gtttctaatc
1681 tgagcatgca gacacatgca gcccgcttta aaacattctt taactggccc tctagtgttc
1741 tagttaatcc tgagcagctt gcaagtgcgg gtttttatta tgtgggtaac agtgatgatg
1801 tcaaatgctt ttgctgtgat ggtggactca ggtgttggga atctggagat gatccatggg
1861 ttcaacatgc caagtggttt ccaaggtgtg agtacttgat aagaattaaa ggacaggagt
1921 tcatccgtca agttcaagcc agttaccctc atctacttga acagctgcta tccacatcag
1981 acagcccagg agatgaaaat gcagagtcat caattatcca ttttgaacct ggagaagacc
2041 attcagaaga tgcaatcatg atgaatactc ctgtgattaa tgctgccgtg gaaatgggct
2101 ttagtagaag cctggtaaaa cagacagttc agagaaaaat cctagcaact ggagagaatt
2161 atagactagt caatgatctt gtgttggact tactcaatgc agaagatgaa ataagggaag
2221 aggagagaga aagagcaact gaggaaaaag aatcaaatga attaaataat cttggtcatc
2281 ctgataataa agagcaaaca actgaccagc ctttggcgaa ggacaaggtt gcccttttga
2341 taggaaatat gaattaccgg gagcacccca agctcaaagc tcctttggtg gatgtgtacg
2401 aattgactaa cttactgaga cagctggact tcaaagtggt ttcactgttg gatcttactg
2461 aatatgagat gcgtaatgct gtggatgagt ttttactcct tttagacaag ggagtatatg
2521 ggttattata ttatgcagga catggttatg aaaattttgg gaacagcttc atggtccccg
2581 ttgatgctcc aaatccatat aggtctgaaa attgtctgtg tgtacaaaat atactgaaat
2641 tgatgcaaga aaaagaaact ggacttaatg tgttcttatt ggatatgtgt aggaaaagaa
2701 atgactacga tgataccatt ccaatcttgg atgcactaaa agtcaccgcc aatattgtgt
2761 ttggatatgc cacgtgtcaa ggagctgaag cttttgaaat ccagcattct ggattggcaa
2821 atggaatctt tatgaaattt ttaaaagaca gattattaga agataagaaa atcactgtgt
2881 tactggatga agttgcagaa gatatgggta agtgtcacct taccaaaggc aaacaggctc
2941 tagagattcg aagtagttta tctgagaaga gagcacttac tgatccaata cagggaacag
3001 aatattctgc tgaatctctt gtgcggaatc tacagtgggc caaggctcat gaacttccag
3061 aaagtatgtg tcttaagttt gactgtggtg ttcagattca attaggattt gcagctgagt
3121 tttccaatgt catgatcatc tatacaagta tagtttacaa accaccggag ataataatgt
3181 gtgatgccta cgttactgat tttccacttg atctagatat tgatccaaaa gatgcaaata
3241 aaggcacgcc tgaagaaact ggcagctact tggtatcaaa ggatcttccc aagcattgcc
3301 tctataccag actcagttca ctgcaaaaat taaaggaaca tctagtcttc acagtatgtt
3361 tatcatatca gtactcagga ttggaagata ctgtagagga caagcaggaa gtgaatgttg
3421 ggaaacctct cattgctaaa ttagacatgc atcgaggttt gggaaggaag acttgctttc
3481 aaacttgtct tatgtctaat ggtccttacc agagttctgc agccacctca ggaggagcag
3541 ggcattatca ctcattgcaa gacccattcc atggtgttta ccattcacat cctggtaatc
3601 caagtaatgt tacaccagca gatagctgtc attgcagccg gactccagat gcatttattt
3661 caagtttcgc tcaccatgct tcatgtcatt ttagtagaag taatgtgcca gtagagacaa
3721 ctgatgaaat accatttagt ttctctgaca ggctcagaat ttctgaaaaa gggcccttcg
3781 aacaaaaact catctcagaa gaggatctga atatgcatac cggtcatcat caccatcacc
3841 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
3901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
3961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
4021 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
4081 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
4381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
4441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
4501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
4561 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
4621 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
4681 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
4741 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
4801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
4861 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
4921 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
4981 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
5041 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
5101 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
5161 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
5221 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
5281 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
5341 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
5401 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
5461 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
5521 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
5581 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
5641 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
5701 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
5761 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
5821 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
5881 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
5941 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
6001 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
6061 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
6121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
6181 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
6241 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
6301 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
6361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
6421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
6481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
6541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
6601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
6661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
6721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
6781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
6841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
6901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
6961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
7021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
7081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
7141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
7201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
7261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
7321 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
7381 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
7441 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
7501 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
7561 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
7621 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
7681 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
7741 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
7801 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
7861 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
7921 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
7981 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
8041 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
8101 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
8161 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
8221 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//