LOCUS       LT726918                7082 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCAGGS-Flag-IKKalpha-W740A, complete
            sequence.
ACCESSION   LT726918
VERSION     LT726918.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCAGGS-Flag-IKKalpha-W740A
  ORGANISM  Mammalian expression vector pCAGGS-Flag-IKKalpha-W740A
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Mammalian expression vector
                     pCAGGS-Flag-IKKalpha-W740A"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 5491."
                     /db_xref="taxon:1944851"
     misc_feature    13..36
                     /note="FLAG epitope"
     CDS             43..2280
                     /transl_table=11
                     /note="hIKKalpha-W740A"
                     /protein_id="SJL86937.1"
                     /translation="MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIK
                     SCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGD
                     LRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKII
                     HKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGY
                     RPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWL
                     QLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESL
                     HSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKT
                     VYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA
                     AMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKML
                     KAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAID
                     LYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPK
                     VEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAW
                     LPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDA
                     SWLTE"
     misc_feature    2260..2265
                     /note="W740A"
     regulatory      2368..2459
                     /note="beta-globin polyA"
                     /regulatory_class="terminator"
     misc_feature    2460..2816
                     /note="3' FR of beta-globin"
     regulatory      complement(2894..2976)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      3118..3193
                     /note="SV40 ori"
     regulatory      3224..3298
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     regulatory      complement(3224..3298)
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     rep_origin      complement(3413..3814)
                     /note="pMB1 ori"
     CDS             complement(4355..5215)
                     /transl_table=11
                     /note="ampicillin"
                     /protein_id="SJL86938.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      5355..5732
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     regulatory      5738..7079
                     /note="AG promoter"
                     /regulatory_class="promoter"
     misc_feature    6107..7031
                     /note="intron AG promoter"
BASE COUNT         1705 a         1693 c         1885 g         1799 t
ORIGIN      
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg
       61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc
      121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt
      181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag
      241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg
      301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg
      361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt
      421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa
      481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg
      541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag
      601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc
      661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag
      721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa
      781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt
      841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag
      901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac
      961 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt
     1021 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga
     1081 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa
     1141 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg
     1201 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt
     1261 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg
     1321 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag
     1381 ggacaaaggg cagcaatgtt aagtcttctt agatataatg ctaacttaac aaaaatgaag
     1441 aacactttga tctcagcatc acaacaactg aaagctaaat tggagttttt tcacaaaagc
     1501 attcagcttg acttggagag atacagcgag cagatgacgt atgggatatc ttcagaaaaa
     1561 atgctaaaag catggaaaga aatggaagaa aaggccatcc actatgctga ggttggtgtc
     1621 attggatacc tggaggatca gattatgtct ttgcatgctg aaatcatgga gctacagaag
     1681 agcccctatg gaagacgtca gggagacttg atggaatctc tggaacagcg tgccattgat
     1741 ctatataagc agttaaaaca cagaccttca gatcactcct acagtgacag cacagagatg
     1801 gtgaaaatca ttgtgcacac tgtgcagagt caggaccgtg tgctcaagga gctgtttggt
     1861 catttgagca agttgttggg ctgtaagcag aagattattg atctactccc taaggtggaa
     1921 gtggccctca gtaatatcaa agaagctgac aatactgtca tgttcatgca gggaaaaagg
     1981 cagaaagaaa tatggcatct ccttaaaatt gcctgtacac agagttctgc ccggtccctt
     2041 gtaggatcca gtctagaagg tgcagtaacc cctcagacat cagcatggct gcccccgact
     2101 tcagcagaac atgatcattc tctgtcatgt gtggtaactc ctcaagatgg ggagacttca
     2161 gcacaaatga tagaagaaaa tttgaactgc cttggccatt taagcactat tattcatgag
     2221 gcaaatgagg aacagggcaa tagtatgatg aatcttgatg ccagctggtt aacagaatga
     2281 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     2341 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     2401 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     2461 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     2521 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     2581 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     2641 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     2701 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     2761 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     2821 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc
     2941 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3001 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     3061 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3121 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3181 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
     3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     3361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3481 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt
     3541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4321 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4441 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4501 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5281 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5341 cacctgggtc gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     5401 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     5461 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     5521 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     5581 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     5641 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     5701 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     5761 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     5821 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg
     5881 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc
     5941 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
     6001 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc
     6061 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     6121 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     6181 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     6241 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     6301 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     6361 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     6421 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     6481 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     6541 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     6601 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc
     6661 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     6721 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     6781 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     6841 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     6901 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     6961 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     7021 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     7081 aa
//