LOCUS LT726916 6980 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-Flag-IKKalpha-deltaLZ, complete
sequence.
ACCESSION LT726916
VERSION LT726916.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-Flag-IKKalpha-deltaLZ
ORGANISM Mammalian expression vector pCAGGS-Flag-IKKalpha-deltaLZ
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6980)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6980
/organism="Mammalian expression vector
pCAGGS-Flag-IKKalpha-deltaLZ"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5489."
/db_xref="taxon:1944844"
misc_feature 13..36
/note="FLAG epitope"
CDS 43..2178
/transl_table=11
/note="hIKKalpha-deltaLZ"
/protein_id="SJL86933.1"
/translation="MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIK
SCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGD
LRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKII
HKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGY
RPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWL
QLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESL
HSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKT
VYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA
ASIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIM
ELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRV
LKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIAC
TQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNC
LGHLSTIIHEANEEQGNSMMNLDWSWLTE"
regulatory 2266..2357
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 2358..2714
/note="3' FR of beta-globin"
regulatory complement(2792..2874)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 3016..3091
/note="SV40 ori"
regulatory 3122..3196
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3122..3196)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3311..3712)
/note="pMB1 ori"
CDS complement(4253..5113)
/transl_table=11
/note="ampicillin"
/protein_id="SJL86934.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5253..5630
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5636..6977
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 6005..6929
/note="intron AG promoter"
BASE COUNT 1663 a 1673 c 1873 g 1771 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg
61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc
121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt
181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag
241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg
301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg
361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt
421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa
481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg
541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag
601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc
661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag
721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa
781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt
841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag
901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac
961 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt
1021 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga
1081 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa
1141 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg
1201 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt
1261 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg
1321 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag
1381 ggacaaaggg cagctagcat tcagcttgac ttggagagat acagcgagca gatgacgtat
1441 gggatatctt cagaaaaaat gctaaaagca tggaaagaaa tggaagaaaa ggccatccac
1501 tatgctgagg ttggtgtcat tggatacctg gaggatcaga ttatgtcttt gcatgctgaa
1561 atcatggagc tacagaagag cccctatgga agacgtcagg gagacttgat ggaatctctg
1621 gaacagcgtg ccattgatct atataagcag ttaaaacaca gaccttcaga tcactcctac
1681 agtgacagca cagagatggt gaaaatcatt gtgcacactg tgcagagtca ggaccgtgtg
1741 ctcaaggagc tgtttggtca tttgagcaag ttgttgggct gtaagcagaa gattattgat
1801 ctactcccta aggtggaagt ggccctcagt aatatcaaag aagctgacaa tactgtcatg
1861 ttcatgcagg gaaaaaggca gaaagaaata tggcatctcc ttaaaattgc ctgtacacag
1921 agttctgccc ggtcccttgt aggatccagt ctagaaggtg cagtaacccc tcagacatca
1981 gcatggctgc ccccgacttc agcagaacat gatcattctc tgtcatgtgt ggtaactcct
2041 caagatgggg agacttcagc acaaatgata gaagaaaatt tgaactgcct tggccattta
2101 agcactatta ttcatgaggc aaatgaggaa cagggcaata gtatgatgaa tcttgattgg
2161 agttggttaa cagaatgact cgaggaattc actcctcagg tgcaggctgc ctatcagaag
2221 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
2281 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
2341 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
2401 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
2461 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
2521 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
2581 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
2641 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
2701 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
2761 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
2821 gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
2881 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
2941 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
3001 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
3061 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
3121 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
3181 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
3241 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
3301 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
3361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata
3421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
3481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
3541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
3601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
3661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
3721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
3781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
3841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
3901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
3961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
4021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
4081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
4141 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
4261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
4321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
4381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
4441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
4501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
4561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
4621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
4681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
4741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
4801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
4861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
4921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
4981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
5041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
5101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
5161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
5221 catttccccg aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata
5281 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
5341 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
5401 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
5461 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
5521 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
5581 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
5641 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
5701 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc
5761 cccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc
5821 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
5881 gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt
5941 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
6001 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
6061 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
6121 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
6181 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
6241 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
6301 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
6361 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
6421 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
6481 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
6541 ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
6601 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
6661 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
6721 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
6781 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
6841 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
6901 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
6961 ctcatcattt tggcaaagaa
//