LOCUS       LT726914                6173 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCAGGS-Flag-IKKalpha-LHS, complete
            sequence.
ACCESSION   LT726914
VERSION     LT726914.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCAGGS-Flag-IKKalpha-LHS
  ORGANISM  Mammalian expression vector pCAGGS-Flag-IKKalpha-LHS
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Mammalian expression vector
                     pCAGGS-Flag-IKKalpha-LHS"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 5487."
                     /db_xref="taxon:1944850"
     misc_feature    13..36
                     /note="FLAG epitope"
     CDS             43..1371
                     /transl_table=11
                     /note="hIKKalpha-LHS"
                     /protein_id="SJL86929.1"
                     /translation="MDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGI
                     NTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSD
                     CVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLT
                     KMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHY
                     AEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHS
                     YSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADN
                     TVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLS
                     CVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE"
     regulatory      1459..1550
                     /note="beta-globin polyA"
                     /regulatory_class="terminator"
     misc_feature    1551..1907
                     /note="3' FR of beta-globin"
     regulatory      complement(1985..2067)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      2209..2284
                     /note="SV40 ori"
     regulatory      2315..2389
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     regulatory      complement(2315..2389)
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     rep_origin      complement(2504..2905)
                     /note="pMB1 ori"
     CDS             complement(3446..4306)
                     /transl_table=11
                     /note="ampicillin"
                     /protein_id="SJL86930.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4446..4823
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     regulatory      4829..6170
                     /note="AG promoter"
                     /regulatory_class="promoter"
     misc_feature    5198..6122
                     /note="intron AG promoter"
BASE COUNT         1440 a         1525 c         1654 g         1554 t
ORIGIN      
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggatca cattttgaat
       61 ttgaagatag tacacatcct aaatatgact tctgcaaaga taatttcttt tctgttacca
      121 cctgatgaaa gtcttcattc actacagtct cgtattgagc gtgaaactgg aataaatact
      181 ggttctcaag aacttctttc agagacagga atttctctgg atcctcggaa accagcctct
      241 caatgtgttc tagatggagt tagaggctgt gatagctata tggtttattt gtttgataaa
      301 agtaaaactg tatatgaagg gccatttgct tccagaagtt tatctgattg tgtaaattat
      361 attgtacagg acagcaaaat acagcttcca attatacagc tgcgtaaagt gtgggctgaa
      421 gcagtgcact atgtgtctgg actaaaagaa gactatagca ggctctttca gggacaaagg
      481 gcagcaatgt taagtcttct tagatataat gctaacttaa caaaaatgaa gaacactttg
      541 atctcagcat cacaacaact gaaagctaaa ttggagtttt ttcacaaaag cattcagctt
      601 gacttggaga gatacagcga gcagatgacg tatgggatat cttcagaaaa aatgctaaaa
      661 gcatggaaag aaatggaaga aaaggccatc cactatgctg aggttggtgt cattggatac
      721 ctggaggatc agattatgtc tttgcatgct gaaatcatgg agctacagaa gagcccctat
      781 ggaagacgtc agggagactt gatggaatct ctggaacagc gtgccattga tctatataag
      841 cagttaaaac acagaccttc agatcactcc tacagtgaca gcacagagat ggtgaaaatc
      901 attgtgcaca ctgtgcagag tcaggaccgt gtgctcaagg agctgtttgg tcatttgagc
      961 aagttgttgg gctgtaagca gaagattatt gatctactcc ctaaggtgga agtggccctc
     1021 agtaatatca aagaagctga caatactgtc atgttcatgc agggaaaaag gcagaaagaa
     1081 atatggcatc tccttaaaat tgcctgtaca cagagttctg cccggtccct tgtaggatcc
     1141 agtctagaag gtgcagtaac ccctcagaca tcagcatggc tgcccccgac ttcagcagaa
     1201 catgatcatt ctctgtcatg tgtggtaact cctcaagatg gggagacttc agcacaaatg
     1261 atagaagaaa atttgaactg ccttggccat ttaagcacta ttattcatga ggcaaatgag
     1321 gaacagggca atagtatgat gaatcttgat tggagttggt taacagaatg actcgaggaa
     1381 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     1441 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     1501 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     1561 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     1621 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     1681 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     1741 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     1801 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     1861 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
     1921 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1981 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg
     2041 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2101 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2221 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     2281 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     2341 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2401 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     2461 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2521 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2581 gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag
     2641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3121 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt
     4441 cgatcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     4501 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4561 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4621 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4681 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4741 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4801 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     4861 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     4921 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
     4981 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     5041 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     5101 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
     5161 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     5221 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     5281 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     5341 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     5401 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     5461 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     5521 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     5581 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     5641 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     5701 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
     5761 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     5821 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     5881 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     5941 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     6001 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     6061 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     6121 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa
//