LOCUS LT726914 6173 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-Flag-IKKalpha-LHS, complete sequence. ACCESSION LT726914 VERSION LT726914.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-Flag-IKKalpha-LHS ORGANISM Mammalian expression vector pCAGGS-Flag-IKKalpha-LHS other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6173) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6173 /organism="Mammalian expression vector pCAGGS-Flag-IKKalpha-LHS" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5487." /db_xref="taxon:1944850" misc_feature 13..36 /note="FLAG epitope" CDS 43..1371 /transl_table=11 /note="hIKKalpha-LHS" /protein_id="SJL86929.1" /translation="MDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGI NTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSD CVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLT KMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHY AEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHS YSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADN TVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLS CVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE" regulatory 1459..1550 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 1551..1907 /note="3' FR of beta-globin" regulatory complement(1985..2067) /note="lac promoter" /regulatory_class="promoter" rep_origin 2209..2284 /note="SV40 ori" regulatory 2315..2389 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2315..2389) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(2504..2905) /note="pMB1 ori" CDS complement(3446..4306) /transl_table=11 /note="ampicillin" /protein_id="SJL86930.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4446..4823 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4829..6170 /note="AG promoter" /regulatory_class="promoter" misc_feature 5198..6122 /note="intron AG promoter" BASE COUNT 1440 a 1525 c 1654 g 1554 t ORIGIN 1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggatca cattttgaat 61 ttgaagatag tacacatcct aaatatgact tctgcaaaga taatttcttt tctgttacca 121 cctgatgaaa gtcttcattc actacagtct cgtattgagc gtgaaactgg aataaatact 181 ggttctcaag aacttctttc agagacagga atttctctgg atcctcggaa accagcctct 241 caatgtgttc tagatggagt tagaggctgt gatagctata tggtttattt gtttgataaa 301 agtaaaactg tatatgaagg gccatttgct tccagaagtt tatctgattg tgtaaattat 361 attgtacagg acagcaaaat acagcttcca attatacagc tgcgtaaagt gtgggctgaa 421 gcagtgcact atgtgtctgg actaaaagaa gactatagca ggctctttca gggacaaagg 481 gcagcaatgt taagtcttct tagatataat gctaacttaa caaaaatgaa gaacactttg 541 atctcagcat cacaacaact gaaagctaaa ttggagtttt ttcacaaaag cattcagctt 601 gacttggaga gatacagcga gcagatgacg tatgggatat cttcagaaaa aatgctaaaa 661 gcatggaaag aaatggaaga aaaggccatc cactatgctg aggttggtgt cattggatac 721 ctggaggatc agattatgtc tttgcatgct gaaatcatgg agctacagaa gagcccctat 781 ggaagacgtc agggagactt gatggaatct ctggaacagc gtgccattga tctatataag 841 cagttaaaac acagaccttc agatcactcc tacagtgaca gcacagagat ggtgaaaatc 901 attgtgcaca ctgtgcagag tcaggaccgt gtgctcaagg agctgtttgg tcatttgagc 961 aagttgttgg gctgtaagca gaagattatt gatctactcc ctaaggtgga agtggccctc 1021 agtaatatca aagaagctga caatactgtc atgttcatgc agggaaaaag gcagaaagaa 1081 atatggcatc tccttaaaat tgcctgtaca cagagttctg cccggtccct tgtaggatcc 1141 agtctagaag gtgcagtaac ccctcagaca tcagcatggc tgcccccgac ttcagcagaa 1201 catgatcatt ctctgtcatg tgtggtaact cctcaagatg gggagacttc agcacaaatg 1261 atagaagaaa atttgaactg ccttggccat ttaagcacta ttattcatga ggcaaatgag 1321 gaacagggca atagtatgat gaatcttgat tggagttggt taacagaatg actcgaggaa 1381 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg 1441 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag 1501 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt 1561 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat 1621 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac 1681 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta 1741 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt 1801 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc 1861 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag 1921 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 1981 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg 2041 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 2101 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca 2161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 2221 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 2281 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg 2341 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 2401 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa 2461 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 2521 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 2581 gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag 2641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 2701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 2761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 3001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 3061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3121 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 3181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 3241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 3301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 3361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 3421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 4321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 4381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt 4441 cgatcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 4501 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 4561 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 4621 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 4681 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 4741 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 4801 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 4861 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 4921 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg 4981 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 5041 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 5101 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg 5161 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 5221 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 5281 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 5341 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 5401 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 5461 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 5521 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 5581 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 5641 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 5701 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg 5761 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 5821 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 5881 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 5941 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 6001 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 6061 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 6121 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa //