LOCUS LT726913 6302 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-Flag-IKKalpha-KL, complete
sequence.
ACCESSION LT726913
VERSION LT726913.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-Flag-IKKalpha-KL
ORGANISM Mammalian expression vector pCAGGS-Flag-IKKalpha-KL
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6302)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6302
/organism="Mammalian expression vector
pCAGGS-Flag-IKKalpha-KL"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5486."
/db_xref="taxon:1944849"
misc_feature 13..36
/note="FLAG epitope"
CDS 43..1500
/transl_table=11
/note="hIKKalpha-KL"
/protein_id="SJL86927.1"
/translation="MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIK
SCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGD
LRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKII
HKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGY
RPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWL
QLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESL
HSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKT
VYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA
AMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHK"
regulatory 1588..1679
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1680..2036
/note="3' FR of beta-globin"
regulatory complement(2114..2196)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2338..2413
/note="SV40 ori"
regulatory 2444..2518
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2444..2518)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2633..3034)
/note="pMB1 ori"
CDS complement(3575..4435)
/transl_table=11
/note="ampicillin"
/protein_id="SJL86928.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4575..4952
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4958..6299
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5327..6251
/note="intron AG promoter"
BASE COUNT 1461 a 1540 c 1690 g 1611 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg
61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc
121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt
181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag
241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg
301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg
361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt
421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa
481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg
541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag
601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc
661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag
721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa
781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt
841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag
901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac
961 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt
1021 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga
1081 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa
1141 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg
1201 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt
1261 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg
1321 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag
1381 ggacaaaggg cagcaatgtt aagtcttctt agatataatg ctaacttaac aaaaatgaag
1441 aacactttga tctcagcatc acaacaactg aaagctaaat tggagttttt tcacaaatga
1501 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
1561 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
1621 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
1681 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
1741 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
1801 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
1861 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
1921 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
1981 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
2041 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
2101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc
2161 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
2221 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
2281 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
2341 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
2401 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
2461 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
2521 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
2581 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
2641 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
2701 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt
2761 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
2821 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
2881 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
2941 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
3001 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
3061 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
3121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
3181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
3241 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
3301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
3361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
3421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
3481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
3541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
3601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
3661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
3721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
3781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
3841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
3901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
3961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
4021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
4081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
4141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
4201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
4261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
4321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
4381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
4441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
4501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
4561 cacctgggtc gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
4621 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
4681 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
4741 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
4801 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
4861 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
4921 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
4981 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
5041 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg
5101 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc
5161 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
5221 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc
5281 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
5341 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
5401 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
5461 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
5521 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
5581 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
5641 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
5701 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
5761 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
5821 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc
5881 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
5941 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
6001 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
6061 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
6121 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
6181 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
6241 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
6301 aa
//