LOCUS LT726912 5756 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-Flag-IKKalpha-K, complete
sequence.
ACCESSION LT726912
VERSION LT726912.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-Flag-IKKalpha-K
ORGANISM Mammalian expression vector pCAGGS-Flag-IKKalpha-K
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5756)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5756
/organism="Mammalian expression vector
pCAGGS-Flag-IKKalpha-K"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5485."
/db_xref="taxon:1944848"
misc_feature 13..36
/note="FLAG epitope"
CDS 43..954
/transl_table=11
/note="hIKKalpha-K"
/protein_id="SJL86925.1"
/translation="MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIK
SCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGD
LRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKII
HKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGY
RPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWL
QLMLNWDPQQRGGPVDLTLKQPRCFVL"
regulatory 1042..1133
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1134..1490
/note="3' FR of beta-globin"
regulatory complement(1568..1650)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1792..1867
/note="SV40 ori"
regulatory 1898..1972
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1898..1972)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2087..2488)
/note="pMB1 ori"
CDS complement(3029..3889)
/transl_table=11
/note="ampicillin"
/protein_id="SJL86926.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4029..4406
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4412..5753
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4781..5705
/note="intron AG promoter"
BASE COUNT 1283 a 1447 c 1583 g 1443 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg
61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc
121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt
181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag
241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg
301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg
361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt
421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa
481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg
541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag
601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc
661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag
721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa
781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt
841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag
901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt atgactcgag
961 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
1021 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
1081 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
1141 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
1201 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
1261 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
1321 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
1381 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
1441 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
1501 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
1561 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga
1621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
1681 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
1741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
1801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
1861 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
1921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
1981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
2041 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
2101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
2161 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa
2221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
2281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
2341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
2401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
2461 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
2521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
2581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
2641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
2701 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
2761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
2821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
2881 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
2941 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
3001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
3061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
3121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
3181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
3241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
3301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
3361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
3421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
3481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
3541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
3601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
3661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
3721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
3781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
3841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
3901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
3961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
4021 ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
4081 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
4141 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
4201 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
4261 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
4321 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
4381 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
4441 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
4501 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga
4561 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg
4621 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
4681 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg
4741 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
4801 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
4861 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
4921 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
4981 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
5041 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
5101 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
5161 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
5221 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
5281 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga
5341 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
5401 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
5461 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
5521 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
5581 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
5641 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
5701 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa
//