LOCUS       LT726909                8251 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Vector pCal-n-MK, complete sequence.
ACCESSION   LT726909
VERSION     LT726909.1
KEYWORDS    .
SOURCE      Vector pCal-n-MK
  ORGANISM  Vector pCal-n-MK
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Vector pCal-n-MK"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 5466."
                     /db_xref="taxon:1945272"
     regulatory      complement(213..260)
                     /note="T7 terminator"
                     /regulatory_class="terminator"
     CDS             complement(<331..2802)
                     /transl_table=11
                     /note="hMLT"
                     /protein_id="SJL86917.1"
                     /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSEL
                     LDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCT
                     VTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQW
                     FKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRS
                     VDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYM
                     VPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDEAVECTEDELNNLGHPDNKEQT
                     TDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMR
                     NAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQ
                     EKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLAN
                     GIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQG
                     TEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPE
                     IIMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHL
                     VFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSS
                     AATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHF
                     SRSNVPVETTDEIPFSFSDRLRISEK"
     misc_feature    complement(853..855)
                     /note="PCR artefact"
     misc_feature    complement(1315..1317)
                     /note="stability creator"
     misc_feature    complement(2797..2799)
                     /note="Kozak"
     misc_feature    complement(2836..2853)
                     /note="thrombin recognition site"
     misc_feature    complement(2854..2931)
                     /note="calmodulin-binding peptide tag"
     misc_feature    complement(2935..2979)
                     /note="5' UTR of T7 gene 10, incl. RBS"
     regulatory      complement(2981..3022)
                     /note="T7 promoter with lac operator"
                     /regulatory_class="promoter"
     regulatory      3331..3408
                     /note="lacI promoter"
                     /regulatory_class="promoter"
     CDS             3409..4491
                     /transl_table=11
                     /note="lacI"
                     /protein_id="SJL86918.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     CDS             5803..5994
                     /transl_table=11
                     /note="rop"
                     /protein_id="SJL86919.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     rep_origin      complement(6240..6642)
                     /note="pMB1 ori"
     CDS             complement(7183..8043)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL86920.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         1985 a         2155 c         2031 g         2080 t
ORIGIN      
        1 ttgtcatgtt tgacagctta tcttcgatta gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
      301 cgggctttgt tagcagccgg atctaagctc tttttcagaa attctgagcc tgtcagagaa
      361 actaaatggt atttcatcag ttgtctctac tggcacatta cttctactaa aatgacatga
      421 agcatggtga gcgaaacttg aaataaatgc atctggagtc cggctgcaat gacagctatc
      481 tgctggtgta acattacttg gattaccagg atgtgaatgg taaacaccat ggaatgggtc
      541 ttgcaatgag tgataatgcc ctgctcctcc tgaggtggct gcagaactct ggtaaggacc
      601 attagacata agacaagttt gaaagcaagt cttccttccc aaacctcgat gcatgtctaa
      661 tttagcaatg agaggtttcc caacattcac ttcctgcttg tcctctacag tatcttccaa
      721 tcctgagtac tgatatgata aacatactgt gaagactaga tgttccttta atttttgcag
      781 tgaactgagt ctggtataga ggcaatgctt gggaagatcc tttgatacca agtagctgcc
      841 agtttcttca ggcgtgcctt tatttgcatc ttttggatca atatctagat caagtggaaa
      901 atcagtaacg taggcatcac acattattat ctccggtggt ttgtaaacta tacttgtata
      961 gatgatcatg acattggaaa actcagctgc aaatcctaat tgaatctgaa caccacagtc
     1021 aaacttaaga cacatacttt ctggaagttc atgagccttg gcccactgta gattccgcac
     1081 aagagattca gcagaatatt ctgttccctg tattggatca gtaagtgctc tcttctcaga
     1141 taaactactt cgaatctcta gagcctgttt gcctttggta aggtgacact tacccatatc
     1201 ttctgcaact tcatccagta acacagtgat tttcttatct tctaataatc tgtcttttaa
     1261 aaatttcata aagattccat ttgccaatcc agaatgctgg atttcaaaag cttcagctcc
     1321 ttgacacgtg gcatatccaa acacaatatt ggcggtgact tttagtgcat ccaagattgg
     1381 aatggtatca tcgtagtcat ttcttttcct acacatatcc aataagaaca cattaagtcc
     1441 agtttctttt tcttgcatca atttcagtat attttgtaca cacagacaat tttcagacct
     1501 atatggattt ggagcatcaa cggggaccat gaagctgttc ccaaaatttt cataaccatg
     1561 tcctgcataa tataataacc catatactcc cttgtctaaa aggagtaaaa actcatccac
     1621 agcattacgc atctcatatt cagtaagatc caacagtgaa accactttga agtccagctg
     1681 tctcagtaag ttagtcaatt cgtacacatc caccaaagga gctttgagct tggggtgctc
     1741 ccggtaattc atatttccta tcaaaagggc aaccttgtcc ttcgccaaag gctggtcagt
     1801 tgtttgctct ttattatcag gatgaccaag attatttaat tcatcttcag tgcactccac
     1861 tgcctcatct gttcttccta tgatgatttc taccttcttg ctatcttgac tgtctcgatc
     1921 attatataca tgacaccagt aggttccttg gtgttccaaa tccacataag gcaccatgta
     1981 tagctttttg gtctcatgtg ttaatggtaa ttcatttttg aaccactggt agtgaggaat
     2041 agggcttcca acagcaacac actgtaaaac caatgtgctg cctggcatca gcttttggga
     2101 agttggttca acacagattt gcaacttgga ttcagagacg ccatcaacac ttctctggaa
     2161 gctctctggg atgtcgcaaa catccagctg tgaccactgg ctgaattcaa aggtgaaatt
     2221 gttattaact cgacagacat aaaagcctgc atcttttaca tgcactgcat taaaaataag
     2281 ctctgatgta tttccatttg gaatctcttt attcattttg aaccactgat attgaacaaa
     2341 aggatgtcca gttgcccggc aacacagttt cacaaactgt ccagccaaga ctgcctttga
     2401 ctctgggttt acagtaatct ttattcctgg ggggctgaga agctgaagaa cttcagtgtg
     2461 ttccatagcc tgcaggaaat cactcaattc tgtgactgtg caaccttttt cacccattaa
     2521 cttcagcaga cacaggctgg ggcttccttc aggctccagt accttaagag aacactgctc
     2581 caggtctagg caactgaggc ggaggcgccc gcgactcccc gccagctccg ccagtctcct
     2641 ccagccccgg ccctcgggcg cctgatccag gagctcgctg agcctccgca gcagcggctc
     2701 ccgcaggcgg ttgagggtcg cgccggccgg aggggcgagc agcggccccg tgggggcggc
     2761 cgagggcggc agggcctgta gcgggtcccc caacagcgcc atggtggcag gatccatgga
     2821 gtctagaatt cccggggatc cacgcggaac cagaagtgcc ccggaggatg agattttctt
     2881 aaagcggttg gctgctgaga cggctatgaa attctttttc catcgtcgct tcatatgtat
     2941 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat
     3001 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac
     3061 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     3121 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     3181 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     3241 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     3301 agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc
     3361 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta
     3421 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg
     3481 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag
     3541 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt
     3601 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa
     3661 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc
     3721 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt
     3781 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg
     3841 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa
     3901 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg
     3961 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat
     4021 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc
     4081 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt
     4141 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt
     4201 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg
     4261 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg
     4321 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg
     4381 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     4441 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     4501 aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc
     4561 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     4621 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     4681 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     4741 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     4801 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     4861 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     4921 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     4981 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac
     5041 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     5101 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     5161 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
     5221 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     5281 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg
     5341 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     5401 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     5461 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     5521 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     5581 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     5641 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     5701 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     5761 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
     5821 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc
     5881 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat
     5941 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6001 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6061 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     6121 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     6181 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     6241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6841 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
     7501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     7801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8161 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     8221 gcgtatcacg aggccctttc gtcttcaaga a
//