LOCUS LT726906 7575 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pCal-c-AK, complete sequence.
ACCESSION LT726906
VERSION LT726906.1
KEYWORDS .
SOURCE Vector pCal-c-AK
ORGANISM Vector pCal-c-AK
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7575)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7575
/organism="Vector pCal-c-AK"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5463."
/db_xref="taxon:1945268"
regulatory complement(213..260)
/note="T7 terminator"
/regulatory_class="terminator"
misc_feature complement(325..402)
/note="calmodulin-binding peptide tag"
misc_feature complement(409..426)
/note="thrombin recognition site"
CDS complement(<433..2244)
/transl_table=11
/note="hAPI2"
/protein_id="SJL86904.1"
/translation="MDIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVP
VSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSV
NNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRS
SYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPK
DNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQL
ASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQV
QASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSR
SLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKN
RMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAAT
VFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVS
IVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS"
misc_feature complement(2239..2241)
/note="Kozak"
misc_feature complement(2261..2303)
/note="5' UTR of T7 gene 10, incl. RBS"
regulatory complement(2305..2346)
/note="T7 promoter with lac operator"
/regulatory_class="promoter"
regulatory 2655..2732
/note="lacI promoter"
/regulatory_class="promoter"
CDS 2733..3815
/transl_table=11
/note="lacI"
/protein_id="SJL86905.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
CDS 5127..5318
/transl_table=11
/note="rop"
/protein_id="SJL86906.1"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
ADELYRSCLARFGDDGENL"
rep_origin complement(5564..5966)
/note="pMB1 ori"
CDS complement(6507..7367)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86907.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1828 a 1948 c 1865 g 1934 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atcaaagtgc cccggaggat gagattttct taaagcggtt
361 ggctgctgag acggctatga aattcttttt ccatcgtcgc ttggtaccat tcccacgtgg
421 atacatggat cctgaaagaa atgtacgaac tgtacccttg attgtactcc tacaaatagg
481 acactttctt aaagaaggag cacaatcttt gcatactact agatgaccac aaggaataaa
541 cactatggac acttctttgt ccatacacac tttacatgtt ctttcttctt gtagtctccg
601 caattgttct tccactggta gatctgaaac atcttctgtg ggaatatatt ttatgtcctg
661 ttgcacaaat aaatgctcat ataacacagc ttcagcttct tgcagagagt ttctgaatac
721 agtggctgca atatttcctt ttactaaaat cgtatcaatc agttctcttg cttgtaaaga
781 cgtctgtgtc ttctgtttaa taacatcatg ttcttgttca ttaataattc cggcagttag
841 tagactatcc aggattggaa ttacacaagt caaatgttga aaaagtgcca ttctattctt
901 ccggattaat aataaatcat ttgattcttt ttcctcagtt gctctttctc tctcctcttc
961 ccttatttca tcttctgcat tgagtaagtc taacacaaga tcattgacta gtctataatt
1021 ctctccagtt gctaggattt ttctctgaac tgtctgtttt accaggcttc tactaaagcc
1081 catttccacg gcagcattaa tcacaggagt attcatcatg attgcatctt ctgaatggtc
1141 ttctccaggt tcaaaatgga taattgatga ctctgcattt tcatctcctg ggctgtctga
1201 tgtggatagc agctgttcaa gtagatgagg gtaactggct tgaacttgac ggatgaactc
1261 ctgtccttta attcttatca agtactcaca ccttggaaac cacttggcat gttgaaccca
1321 tggatcatct ccagattccc aacacctgag tccaccatca cagcaaaagc atttgacatc
1381 atcactgtta cccacataat aaaaacccgc acttgcaagc tgctcaggat taactagaac
1441 actagagggc cagttaaaga atgttttaaa gcgggctgca tgtgtctgca tgctcagatt
1501 agaaactgtg tatcttgaag tgtcttgaag ctgattttct ataaatgggc atttgggaaa
1561 atgtctcagg tgttctgaca tagcattatc cttcggttcc caattgctca attttccacc
1621 acaggcaaag caagccactc tgtctccagg tcctatgtag taaaagcctg cttttgccag
1681 atctgttggc gacagaaaag tcaatggcca tgtctgaaaa gtaagtaatc tggcattttc
1741 gttattcatt gcacagtggt aggaacttct catcaaggca gaaaaatctt gatttgctct
1801 ggagtttaca ggatttgatg gagagtttga ataagagcca cggaaatatc cactgttttc
1861 tgtacccgga agtaatgagt gtgtggaatt tgttactgaa gaaggaaaag taggctgaga
1921 ggtagcttcc aagttgttaa cggaatttag actctgaacg aatctgcagc taggatacaa
1981 ctttttatgc ttttcagtag gactgtctcc tcttttccag ttatccagca tcaggccaca
2041 acagaagcat ttgaccttgt cattcacacc agtgtaatag aaaccagcac gagcaagact
2101 cctttctgag acaggaaccc cagcaggaaa agtggaatac gtagacattc ggtacagttc
2161 acatgacaag tcgtatttca gttcaaacgt gttggcgctt ttcatcaaat ttgataagaa
2221 tatgctgttt tctactatgt ccatggtggc aggatccatg gtatatctcc ttcttaaagt
2281 taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc
2341 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat
2401 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
2461 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
2521 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
2581 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
2641 gcgtcgagat cccggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc
2701 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg
2761 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
2821 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
2881 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
2941 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
3001 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
3061 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
3121 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
3181 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
3241 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
3301 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
3361 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
3421 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
3481 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
3541 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
3601 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
3661 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
3721 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
3781 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag
3841 ctcactcatt aggcaccggg atctcgaccg atgcccttga gagccttcaa cccagtcagc
3901 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
3961 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
4021 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
4081 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
4141 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
4201 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
4261 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
4321 gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc
4381 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
4441 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
4501 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
4561 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
4621 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
4681 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
4741 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
4801 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
4861 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
4921 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc
4981 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat
5041 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca
5101 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg
5161 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg
5221 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct
5281 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
5341 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
5401 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
5461 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt
5521 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
5581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
5641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
5701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
5761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
5821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
5881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
5941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
6001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
6061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
6121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
6181 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
6241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
6301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
6361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
6421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
6481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
6541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
6601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
6661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
6721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
6781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
6841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
6901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
6961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
7021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
7081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
7141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
7201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
7261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
7321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
7381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
7441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
7501 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
7561 tttcgtcttc aagaa
//