LOCUS LT726904 7591 bp DNA circular SYN 06-FEB-2017 DEFINITION Vector pCal-n-AK, complete sequence. ACCESSION LT726904 VERSION LT726904.1 KEYWORDS . SOURCE Vector pCal-n-AK ORGANISM Vector pCal-n-AK other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7591) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7591 /organism="Vector pCal-n-AK" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5461." /db_xref="taxon:1945271" regulatory complement(213..260) /note="T7 terminator" /regulatory_class="terminator" CDS complement(<331..2142) /transl_table=11 /note="hAPI2" /protein_id="SJL86895.1" /translation="MDIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVP VSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSV NNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRS SYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPK DNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQL ASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQV QASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSR SLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKN RMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAAT VFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVS IVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS" misc_feature complement(2137..2139) /note="Kozak" misc_feature complement(2176..2193) /note="thrombin recognition site" misc_feature complement(2194..2271) /note="calmodulin-binding peptide tag" misc_feature complement(2275..2319) /note="5' UTR of T7 gene 10, incl. RBS" regulatory complement(2321..2362) /note="T7 promoter with lac operator" /regulatory_class="promoter" regulatory 2671..2748 /note="lacI promoter" /regulatory_class="promoter" CDS 2749..3831 /transl_table=11 /note="lacI" /protein_id="SJL86896.1" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" CDS 5143..5334 /transl_table=11 /note="rop" /protein_id="SJL86897.1" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH ADELYRSCLARFGDDGENL" rep_origin complement(5580..5982) /note="pMB1 ori" CDS complement(6523..7383) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86898.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1831 a 1951 c 1871 g 1938 t ORIGIN 1 ttgtcatgtt tgacagctta tcttcgatta gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 301 cgggctttgt tagcagccgg atctaagctc tgaaagaaat gtacgaactg tacccttgat 361 tgtactccta caaataggac actttcttaa agaaggagca caatctttgc atactactag 421 atgaccacaa ggaataaaca ctatggacac ttctttgtcc atacacactt tacatgttct 481 ttcttcttgt agtctccgca attgttcttc cactggtaga tctgaaacat cttctgtggg 541 aatatatttt atgtcctgtt gcacaaataa atgctcatat aacacagctt cagcttcttg 601 cagagagttt ctgaatacag tggctgcaat atttcctttt actaaaatcg tatcaatcag 661 ttctcttgct tgtaaagacg tctgtgtctt ctgtttaata acatcatgtt cttgttcatt 721 aataattccg gcagttagta gactatccag gattggaatt acacaagtca aatgttgaaa 781 aagtgccatt ctattcttcc ggattaataa taaatcattt gattcttttt cctcagttgc 841 tctttctctc tcctcttccc ttatttcatc ttctgcattg agtaagtcta acacaagatc 901 attgactagt ctataattct ctccagttgc taggattttt ctctgaactg tctgttttac 961 caggcttcta ctaaagccca tttccacggc agcattaatc acaggagtat tcatcatgat 1021 tgcatcttct gaatggtctt ctccaggttc aaaatggata attgatgact ctgcattttc 1081 atctcctggg ctgtctgatg tggatagcag ctgttcaagt agatgagggt aactggcttg 1141 aacttgacgg atgaactcct gtcctttaat tcttatcaag tactcacacc ttggaaacca 1201 cttggcatgt tgaacccatg gatcatctcc agattcccaa cacctgagtc caccatcaca 1261 gcaaaagcat ttgacatcat cactgttacc cacataataa aaacccgcac ttgcaagctg 1321 ctcaggatta actagaacac tagagggcca gttaaagaat gttttaaagc gggctgcatg 1381 tgtctgcatg ctcagattag aaactgtgta tcttgaagtg tcttgaagct gattttctat 1441 aaatgggcat ttgggaaaat gtctcaggtg ttctgacata gcattatcct tcggttccca 1501 attgctcaat tttccaccac aggcaaagca agccactctg tctccaggtc ctatgtagta 1561 aaagcctgct tttgccagat ctgttggcga cagaaaagtc aatggccatg tctgaaaagt 1621 aagtaatctg gcattttcgt tattcattgc acagtggtag gaacttctca tcaaggcaga 1681 aaaatcttga tttgctctgg agtttacagg atttgatgga gagtttgaat aagagccacg 1741 gaaatatcca ctgttttctg tacccggaag taatgagtgt gtggaatttg ttactgaaga 1801 aggaaaagta ggctgagagg tagcttccaa gttgttaacg gaatttagac tctgaacgaa 1861 tctgcagcta ggatacaact ttttatgctt ttcagtagga ctgtctcctc ttttccagtt 1921 atccagcatc aggccacaac agaagcattt gaccttgtca ttcacaccag tgtaatagaa 1981 accagcacga gcaagactcc tttctgagac aggaacccca gcaggaaaag tggaatacgt 2041 agacattcgg tacagttcac atgacaagtc gtatttcagt tcaaacgtgt tggcgctttt 2101 catcaaattt gataagaata tgctgttttc tactatgtcc atggtggcag gatccatgga 2161 gtctagaatt cccggggatc cacgcggaac cagaagtgcc ccggaggatg agattttctt 2221 aaagcggttg gctgctgaga cggctatgaa attctttttc catcgtcgct tcatatgtat 2281 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat 2341 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac 2401 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca 2461 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg 2521 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat 2581 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg 2641 agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc 2701 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta 2761 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 2821 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 2881 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 2941 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 3001 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 3061 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 3121 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 3181 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 3241 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 3301 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 3361 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 3421 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 3481 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 3541 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 3601 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 3661 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 3721 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 3781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 3841 aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc 3901 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 3961 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt 4021 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg 4081 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga 4141 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt 4201 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg 4261 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct 4321 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac 4381 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc 4441 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac 4501 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat 4561 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg 4621 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg 4681 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact 4741 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg 4801 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa 4861 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg 4921 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt 4981 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac 5041 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc 5101 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa 5161 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc 5221 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat 5281 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 5341 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 5401 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 5461 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 5521 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 5581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 5641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5701 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5761 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5821 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 5881 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 5941 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6001 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6061 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6121 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 6181 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 6241 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 6301 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6361 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6421 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 6541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6601 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6661 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 6721 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 6781 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 6841 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 6901 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 6961 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7021 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7081 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 7141 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7201 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7261 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7321 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 7381 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7501 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 7561 gcgtatcacg aggccctttc gtcttcaaga a //