LOCUS LT726904 7591 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pCal-n-AK, complete sequence.
ACCESSION LT726904
VERSION LT726904.1
KEYWORDS .
SOURCE Vector pCal-n-AK
ORGANISM Vector pCal-n-AK
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7591)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7591
/organism="Vector pCal-n-AK"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5461."
/db_xref="taxon:1945271"
regulatory complement(213..260)
/note="T7 terminator"
/regulatory_class="terminator"
CDS complement(<331..2142)
/transl_table=11
/note="hAPI2"
/protein_id="SJL86895.1"
/translation="MDIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVP
VSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSV
NNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRS
SYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPK
DNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQL
ASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQV
QASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSR
SLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKN
RMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAAT
VFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVS
IVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS"
misc_feature complement(2137..2139)
/note="Kozak"
misc_feature complement(2176..2193)
/note="thrombin recognition site"
misc_feature complement(2194..2271)
/note="calmodulin-binding peptide tag"
misc_feature complement(2275..2319)
/note="5' UTR of T7 gene 10, incl. RBS"
regulatory complement(2321..2362)
/note="T7 promoter with lac operator"
/regulatory_class="promoter"
regulatory 2671..2748
/note="lacI promoter"
/regulatory_class="promoter"
CDS 2749..3831
/transl_table=11
/note="lacI"
/protein_id="SJL86896.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
CDS 5143..5334
/transl_table=11
/note="rop"
/protein_id="SJL86897.1"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
ADELYRSCLARFGDDGENL"
rep_origin complement(5580..5982)
/note="pMB1 ori"
CDS complement(6523..7383)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86898.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1831 a 1951 c 1871 g 1938 t
ORIGIN
1 ttgtcatgtt tgacagctta tcttcgatta gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atctaagctc tgaaagaaat gtacgaactg tacccttgat
361 tgtactccta caaataggac actttcttaa agaaggagca caatctttgc atactactag
421 atgaccacaa ggaataaaca ctatggacac ttctttgtcc atacacactt tacatgttct
481 ttcttcttgt agtctccgca attgttcttc cactggtaga tctgaaacat cttctgtggg
541 aatatatttt atgtcctgtt gcacaaataa atgctcatat aacacagctt cagcttcttg
601 cagagagttt ctgaatacag tggctgcaat atttcctttt actaaaatcg tatcaatcag
661 ttctcttgct tgtaaagacg tctgtgtctt ctgtttaata acatcatgtt cttgttcatt
721 aataattccg gcagttagta gactatccag gattggaatt acacaagtca aatgttgaaa
781 aagtgccatt ctattcttcc ggattaataa taaatcattt gattcttttt cctcagttgc
841 tctttctctc tcctcttccc ttatttcatc ttctgcattg agtaagtcta acacaagatc
901 attgactagt ctataattct ctccagttgc taggattttt ctctgaactg tctgttttac
961 caggcttcta ctaaagccca tttccacggc agcattaatc acaggagtat tcatcatgat
1021 tgcatcttct gaatggtctt ctccaggttc aaaatggata attgatgact ctgcattttc
1081 atctcctggg ctgtctgatg tggatagcag ctgttcaagt agatgagggt aactggcttg
1141 aacttgacgg atgaactcct gtcctttaat tcttatcaag tactcacacc ttggaaacca
1201 cttggcatgt tgaacccatg gatcatctcc agattcccaa cacctgagtc caccatcaca
1261 gcaaaagcat ttgacatcat cactgttacc cacataataa aaacccgcac ttgcaagctg
1321 ctcaggatta actagaacac tagagggcca gttaaagaat gttttaaagc gggctgcatg
1381 tgtctgcatg ctcagattag aaactgtgta tcttgaagtg tcttgaagct gattttctat
1441 aaatgggcat ttgggaaaat gtctcaggtg ttctgacata gcattatcct tcggttccca
1501 attgctcaat tttccaccac aggcaaagca agccactctg tctccaggtc ctatgtagta
1561 aaagcctgct tttgccagat ctgttggcga cagaaaagtc aatggccatg tctgaaaagt
1621 aagtaatctg gcattttcgt tattcattgc acagtggtag gaacttctca tcaaggcaga
1681 aaaatcttga tttgctctgg agtttacagg atttgatgga gagtttgaat aagagccacg
1741 gaaatatcca ctgttttctg tacccggaag taatgagtgt gtggaatttg ttactgaaga
1801 aggaaaagta ggctgagagg tagcttccaa gttgttaacg gaatttagac tctgaacgaa
1861 tctgcagcta ggatacaact ttttatgctt ttcagtagga ctgtctcctc ttttccagtt
1921 atccagcatc aggccacaac agaagcattt gaccttgtca ttcacaccag tgtaatagaa
1981 accagcacga gcaagactcc tttctgagac aggaacccca gcaggaaaag tggaatacgt
2041 agacattcgg tacagttcac atgacaagtc gtatttcagt tcaaacgtgt tggcgctttt
2101 catcaaattt gataagaata tgctgttttc tactatgtcc atggtggcag gatccatgga
2161 gtctagaatt cccggggatc cacgcggaac cagaagtgcc ccggaggatg agattttctt
2221 aaagcggttg gctgctgaga cggctatgaa attctttttc catcgtcgct tcatatgtat
2281 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat
2341 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac
2401 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
2461 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
2521 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
2581 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
2641 agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc
2701 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta
2761 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg
2821 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag
2881 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt
2941 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa
3001 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc
3061 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt
3121 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg
3181 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa
3241 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg
3301 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat
3361 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc
3421 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt
3481 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt
3541 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg
3601 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg
3661 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg
3721 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
3781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
3841 aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc
3901 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
3961 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
4021 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
4081 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
4141 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
4201 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
4261 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
4321 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac
4381 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
4441 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
4501 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
4561 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
4621 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg
4681 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
4741 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
4801 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
4861 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
4921 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
4981 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
5041 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
5101 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
5161 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc
5221 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat
5281 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
5341 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
5401 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
5461 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
5521 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
5581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
5641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
5701 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
5761 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
5821 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
5881 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
5941 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
6001 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
6061 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
6121 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
6181 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
6241 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
6301 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
6361 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
6421 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
6481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
6541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
6601 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
6661 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
6721 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
6781 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
6841 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
6901 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
6961 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
7021 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
7081 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
7141 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
7201 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
7261 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
7321 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
7381 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
7441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
7501 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
7561 gcgtatcacg aggccctttc gtcttcaaga a
//