LOCUS LT726903 9714 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pAS2-mABIN-2, complete sequence. ACCESSION LT726903 VERSION LT726903.1 KEYWORDS . SOURCE Yeast expression vector pAS2-mABIN-2 ORGANISM Yeast expression vector pAS2-mABIN-2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9714) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..9714 /organism="Yeast expression vector pAS2-mABIN-2" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5448." /db_xref="taxon:1945461" rep_origin complement(2..982) /note="2mu ori" regulatory complement(1060..1164) /note="FLP terminator" /regulatory_class="terminator" CDS 1884..2558 /transl_table=11 /note="TRP1" /protein_id="SJL86890.1" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK" rep_origin complement(2661..3116) /note="f1 ori" regulatory complement(3312..3586) /note="CYC1 terminator" /regulatory_class="terminator" CDS complement(3855..4304) /transl_table=11 /note="CYH2" /protein_id="SJL86891.1" /translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA" regulatory 5075..5478 /note="ADH1 promoter" /regulatory_class="promoter" CDS 5501..>5941 /transl_table=11 /note="GALbd" /protein_id="SJL86892.1" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" misc_feature 5957..5983 /note="HA epitope" CDS 6026..7318 /transl_table=11 /note="mABIN-2" /protein_id="SJL86893.1" /translation="MSSGDPRSGRQDGAPRAAAALCGLYHEAGQQLQRLKDQLAARDA LIASLRTRLAALEGHTAPSLVDALLDQVERFREQLRRQEEGASETQLRQEVERLTERL EEKEREMQQLMSQPQHEQEKEVVLLRRSVAEKEKARAASDVLCRSLADETHQLRRTLA ATAHMCQHLAKCLDERQCAQGDAGEKSPAELEQTSSDASGQSVIKKLQEENRLLKQKV THVEDLNAKWQRYDASRDEYVKGLHAQLKRRQVPLEPELMKKEISRLNRQLEEKISDC AEANQELTAMRMSRDTALERVQMLEQQILAYKDDFKSERADRERAHSRIQELEEKIMS LMYQVSQRQDSREPGPCRIHTGNKTAKYLEMDALEHVTPGGWRPESRSQQMEPSAEGG HVCTAQRGQGDLQCPHCLRCFSDEQGEAFLRHLSECCQ" regulatory 7352..7453 /note="ADH1 terminator" /regulatory_class="terminator" regulatory complement(7606..7689) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(7781..8183) /note="pMB1 ori" CDS complement(8724..9584) /transl_table=11 /note="ampicillin" /protein_id="SJL86894.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2691 a 2076 c 2299 g 2648 t ORIGIN 1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 6001 ggccatggag gccccgggga tccgtatgtc gtctggggac ccaaggtctg gtagacagga 6061 cggggccccg cgtgcggccg cagcgctctg tggcctgtac cacgaggccg gccagcaact 6121 acagcgcctg aaggatcagc tggccgcgcg tgacgccctc atcgcgagcc tccgcacccg 6181 cctcgcggct ctggaagggc acacggcgcc gtcactcgtg gacgcacttc tggatcaggt 6241 ggagcgcttc cgtgagcagc tgcgacgaca ggaggaaggc gcttcggaga cccagctgcg 6301 gcaggaagtt gaaagactta cggagcgtct agaggaaaaa gagagggaga tgcaacagct 6361 gatgagccag cctcagcatg agcaagagaa ggaggtagtc ttgcttcggc gaagtgtggc 6421 agagaaggag aaagccaggg ccgccagtga tgttctgtgc cgctccttgg ctgatgagac 6481 ccaccaactg cgcaggacat tggcagccac tgcccacatg tgccaacatc tggccaaatg 6541 tctggatgaa cgacagtgtg cacagggaga cgctggggag aaaagccctg ctgagctaga 6601 gcaaacaagc agcgatgctt ctggccagag tgttattaag aagttacagg aagaaaatcg 6661 actgttaaaa cagaaggtga ctcatgtaga agacctcaat gctaagtggc agcgttatga 6721 tgcaagtagg gacgaatatg tgaaggggtt gcatgcccag ctaaagaggc ggcaggtccc 6781 tctggagcct gagctgatga agaaggagat ttcccgactt aacagacagt tggaggagaa 6841 aataagtgac tgtgcggaag caaaccagga gctgacagcc atgaggatgt cccgggacac 6901 tgcgctggag cgagtgcaga tgctagaaca gcagattctt gcttacaagg atgacttcaa 6961 atcagaaagg gcagatcggg aacgagcgca cagtaggatt caagagctgg aggaaaagat 7021 catgtccttg atgtaccaag tgtcccagag acaggactcc cgggagccag gaccctgtcg 7081 gattcatacg gggaacaaaa ctgccaagta cttagagatg gatgcactgg agcatgtgac 7141 ccctggcggc tggaggcctg agtctaggtc ccaacagatg gaaccttctg cagagggtgg 7201 gcatgtgtgc acagcccaga gaggtcaggg tgaccttcag tgccctcatt gcctgcggtg 7261 cttcagtgat gagcaaggcg aggcattcct caggcacctg tctgagtgct gccaatgaag 7321 atccgtcgac ctgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat 7381 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 7441 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 7501 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca 7561 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 7621 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt 7681 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct ggattaatga 7741 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 7801 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 7861 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 7921 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 7981 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 8041 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 8101 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 8161 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 8221 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 8281 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 8341 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 8401 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 8461 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 8521 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 8581 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 8641 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 8701 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 8761 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 8821 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 8881 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 8941 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 9001 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 9061 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 9121 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 9181 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 9241 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 9301 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 9361 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 9421 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 9481 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 9541 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 9601 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 9661 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acct //