LOCUS LT726902 6751 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pRH020, complete sequence.
ACCESSION LT726902
VERSION LT726902.1
KEYWORDS .
SOURCE Vector pRH020
ORGANISM Vector pRH020
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6751)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6751
/organism="Vector pRH020"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5431."
/db_xref="taxon:1945387"
rep_origin 536..646
/note="RK2/RP4 oriT"
regulatory 968..984
/note="T7 promoter"
/regulatory_class="promoter"
regulatory complement(1050..1174)
/note="attR1"
/regulatory_class="attenuator"
regulatory 1182..1234
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 1283..1942
/transl_table=11
/note="chloramphenicol"
/protein_id="SJL86886.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 2284..2589
/transl_table=11
/note="ccdB"
/protein_id="SJL86887.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 2630..2754
/note="attR2"
/regulatory_class="attenuator"
CDS 2789..>3442
/transl_table=11
/note="GST"
/protein_id="SJL86888.1"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
"
regulatory complement(3673..3689)
/note="T3 promoter"
/regulatory_class="promoter"
regulatory complement(3753..3836)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(3928..4330)
/note="pMB1 ori"
CDS 5650..6309
/transl_table=11
/note="chloramphenicol"
/protein_id="SJL86889.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
BASE COUNT 1737 a 1576 c 1675 g 1763 t
ORIGIN
1 ggggatcggt cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct
61 tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc
121 ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc
181 tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc
241 gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg
301 ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag
361 ccctggccga cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc
421 tcaatcgctc ttcgttcgtc tgaaagcagt acaccttgat aggtgggctg cctttcctgg
481 gtggcttggt ttaatcagcc atccgctggc cttcatccgg tacgccgccg gaggccggcc
541 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga
601 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga
661 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg
721 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaaga
781 tccgtcgaat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
841 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
901 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
961 gtgattgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
1021 cggtatcgat aagcttatcg atcagatcaa caagtttgta caaaaaagct gaacgagaaa
1081 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
1141 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
1201 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt
1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
1681 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
1861 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
1921 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
1981 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
2041 tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc
2101 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
2161 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
2221 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt
2281 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
2341 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
2401 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
2461 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
2521 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
2581 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
2641 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
2701 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatct
2761 ggggtcgacg gatccccggg taattaatat gtcccctata ctaggttatt ggaaaattaa
2821 gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat atgaagagca
2881 tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga
2941 gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt ctatggccat
3001 catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag agcgtgcaga
3061 gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga gaattgcata
3121 tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg aaatgctgaa
3181 aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg taacccatcc
3241 tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa tgtgcctgga
3301 tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac aaattgataa
3361 gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag ccacgtttgg
3421 tggtggcgac catcctccaa aatcggatct ggttccgcgt tgaggcgcgc cactcgaggg
3481 ggggcccggt accaagatgg cctttggtgg gttgaagaag gaaaaagaca gaaacgactt
3541 aattacctac ttgaaaaaag cctgtgagta aacaggcccc ttttcctttg tcgatatcga
3601 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
3661 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
3721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
3781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
3841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
3901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
3961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
4021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
4081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
4141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
4201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
4261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
4321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
4381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
4441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
4501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
4561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
4621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
4681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
4741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
4801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
4861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
4921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
4981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
5041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
5101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
5161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
5221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
5281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
5341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
5401 gatgcttttc tgtgactggt gagtggggat cctctagagt ccgaataaat acctgtgacg
5461 gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg
5521 gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat
5581 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
5641 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
5701 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
5761 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
5821 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
5881 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
5941 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
6001 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
6061 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
6121 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
6181 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
6241 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
6301 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa
6361 taagtgataa taagcggatg aatggcagaa attcggacct gcaggcatgc aactctagag
6421 gatccccact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
6481 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
6541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
6601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
6661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
6721 tgttgaatac tcatactctt cctttttcaa t
//