LOCUS LT726897 6879 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pRH012, complete sequence.
ACCESSION LT726897
VERSION LT726897.1
KEYWORDS .
SOURCE Vector pRH012
ORGANISM Vector pRH012
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6879)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6879
/organism="Vector pRH012"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5423."
/db_xref="taxon:1945382"
rep_origin 536..646
/note="RK2/RP4 oriT"
regulatory 968..984
/note="T7 promoter"
/regulatory_class="promoter"
CDS complement(1319..2134)
/transl_table=11
/note="kanamycin"
/protein_id="SJL86847.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
regulatory complement(2312..2436)
/note="attR1"
/regulatory_class="attenuator"
regulatory 2444..2496
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 2545..3204
/transl_table=11
/note="chloramphenicol"
/protein_id="SJL86848.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 3546..3851
/transl_table=11
/note="ccdB"
/protein_id="SJL86849.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 3892..4016
/note="attR2"
/regulatory_class="attenuator"
CDS 4045..4764
/transl_table=11
/note="EGFP"
/protein_id="SJL86850.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory complement(4804..4820)
/note="T3 promoter"
/regulatory_class="promoter"
regulatory complement(4884..4967)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5059..5461)
/note="pMB1 ori"
CDS complement(6002..6862)
/transl_table=11
/note="ampicillin"
/protein_id="SJL86851.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1735 a 1758 c 1718 g 1668 t
ORIGIN
1 ggggatcggt cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct
61 tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc
121 ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc
181 tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc
241 gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg
301 ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag
361 ccctggccga cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc
421 tcaatcgctc ttcgttcgtc tgaaagcagt acaccttgat aggtgggctg cctttcctgg
481 gtggcttggt ttaatcagcc atccgctggc cttcatccgg tacgccgccg gaggccggcc
541 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga
601 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga
661 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg
721 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaaga
781 tccgtcgaat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
841 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
901 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
961 gtgattgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgacctgca
1021 gggggggggg ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc
1081 tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg
1141 taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg
1201 ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc
1261 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
1321 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
1381 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
1441 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
1501 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
1561 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
1621 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
1681 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
1741 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
1801 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
1861 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
1921 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
1981 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
2041 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
2101 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
2161 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
2221 gattttgaga cacaacgtgg ctttcccccc cccccctgca ggtcgagctc aagcttcgaa
2281 ttctgcagtc gacggtaccg cgggcccatc aacaagtttg tacaaaaaag ctgaacgaga
2341 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
2401 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
2461 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga
2521 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
2581 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
2641 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
2701 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
2761 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
2821 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
2881 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
2941 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
3001 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
3061 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
3121 catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
3181 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
3241 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
3301 agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca
3361 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
3421 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
3481 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acagggactg
3541 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
3601 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
3661 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
3721 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
3781 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
3841 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
3901 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
3961 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat
4021 gggatccacc ggccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
4081 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
4141 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
4201 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
4261 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
4321 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
4381 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
4441 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
4501 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
4561 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
4621 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
4681 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
4741 ggcatggacg agctgtacaa gtaaagcggc cgccaccgcg gtggagctcc agcttttgtt
4801 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
4861 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
4921 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
4981 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
5041 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
5101 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
5161 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
5221 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
5281 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
5341 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
5401 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
5461 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
5521 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
5581 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
5641 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
5701 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
5761 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
5821 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
5881 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
5941 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
6001 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
6061 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
6121 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
6181 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
6241 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
6301 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
6361 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
6421 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
6481 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
6541 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
6601 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
6661 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
6721 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
6781 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
6841 cacggaaatg ttgaatactc atactcttcc tttttcaat
//