LOCUS LT726886 10365 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pAS2-mABIN-1(54-647), complete sequence. ACCESSION LT726886 VERSION LT726886.1 KEYWORDS . SOURCE Yeast expression vector pAS2-mABIN-1(54-647) ORGANISM Yeast expression vector pAS2-mABIN-1(54-647) other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 10365) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..10365 /organism="Yeast expression vector pAS2-mABIN-1(54-647)" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5393." /db_xref="taxon:1945460" rep_origin complement(2..982) /note="2mu ori" regulatory complement(1060..1164) /note="FLP terminator" /regulatory_class="terminator" CDS 1884..2558 /transl_table=11 /note="TRP1" /protein_id="SJL86772.1" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK" rep_origin complement(2661..3116) /note="f1 ori" regulatory complement(3312..3586) /note="CYC1 terminator" /regulatory_class="terminator" CDS complement(3855..4304) /transl_table=11 /note="CYH2" /protein_id="SJL86773.1" /translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA" regulatory 5075..5478 /note="ADH1 promoter" /regulatory_class="promoter" CDS 5501..>5941 /transl_table=11 /note="GALbd" /protein_id="SJL86774.1" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" misc_feature 5957..5983 /note="HA epitope" CDS 6008..7792 /transl_table=11 /note="mABIN-1(54-647)" /protein_id="SJL86775.1" /translation="MEASRLRQKAEELVKDSELSPPTSAPSLVSFDDLAELTGQDTKV QVHPATSTAATTTATATTGNSMEKPEPASKSPSNGASSDFEVVPTEEQNSPETGSHPT NMMDLGPPPPEDSNLKLHLQRLETTLSVCAEEPDHSQLFTHLGRMALEFNRLASKVHK NEQRTSILQTLCEQLRQENEALKAKLDKGLEQRDLAAERLREENTELKKLLMNSSCKE GLCGQPSSPKPEGAGKKGVAGQQQASVMASKVPEAGAFGAAEKKVKLLEQQRMELLEV NKQWDQHFRSMKQQYEQKITELRQKLVDLQKQVTELEAEREQKQRDFDRKLLLAKSKI EMEETDKEQLTAEAKELRQKVRYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQAS PSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEEL KKQVEKLQAQVTLTNAQLKTLKEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGAY PYAYPPMPAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNSRLFHLPEYTWRPPCAGIR NQSSQVMDPPPDRPAEPESADNDCDGPQ" regulatory 8003..8104 /note="ADH1 terminator" /regulatory_class="terminator" regulatory complement(8257..8340) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(8432..8834) /note="pMB1 ori" CDS complement(9375..10235) /transl_table=11 /note="ampicillin" /protein_id="SJL86776.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2874 a 2327 c 2441 g 2723 t ORIGIN 1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 6001 ggccgcgatg gaagcgtcca gactccggca gaaggcagag gagctggtca aggacagcga 6061 gctgtcacca ccgacatctg ccccctcctt ggtctccttt gatgacctgg ctgagctcac 6121 aggacaggat acaaaggtcc aggtacatcc tgctaccagc actgccgcca ccaccaccgc 6181 caccgccacc acgggaaact ccatggagaa gcccgagcca gcctccaaat ctccgtccaa 6241 tggcgcctcc tcggactttg aagtggtccc tactgaggag cagaattcac ccgaaactgg 6301 cagccaccct acgaacatga tggacctggg gcccccaccc ccagaggaca gcaacctgaa 6361 gctccacctg cagcgcctgg agaccaccct tagcgtgtgt gcagaggagc cagaccacag 6421 ccagctcttc acccacctgg gccgcatggc cctcgagttc aacaggttgg cctccaaagt 6481 gcataaaaat gagcagcgca cctccatcct gcagacctta tgtgagcagc tgcgccagga 6541 gaatgaagcc ctgaaggcca agctggacaa gggcctggaa cagcgggatc tggctgctga 6601 gaggctgcgg gaggaaaaca cggagctcaa gaaactgttg atgaacagca gctgcaaaga 6661 gggactctgt gggcagccca gctccccaaa gccagagggt gctggcaaga agggcgtggc 6721 tggacagcag caggccagtg tgatggcgag taaagtccct gaagcggggg cctttggagc 6781 agctgagaag aaagtgaagt tgctagaaca gcaacgcatg gagctgctgg aagtgaacaa 6841 gcagtgggac cagcatttcc ggtccatgaa gcagcagtat gagcagaaga tcacagagct 6901 tcgccagaag ctggtggacc tgcagaaaca ggtaactgag ctggaggccg aacgggagca 6961 gaagcagcgt gactttgacc ggaaactcct cctggccaaa tcgaagatag agatggaaga 7021 gaccgacaag gagcagctga cagcagaggc caaggaactg cgccagaagg tcaggtacct 7081 acaggatcag ctgagcccgc tcacaaggca acgagaatac caggagaagg agatccagcg 7141 gctcaataag gccctggagg aggccctcag catccaggcc tctccatcat ctccgcctgc 7201 agcttttggg agtccagaag gcgttggggg ccatctgagg aagcaggaac tagtgacaca 7261 gaatgagttg ctgaaacagc aggtaaagat ctttgaagag gacttccaga gggaacggag 7321 tgaccgtgaa cgcatgaatg aagagaagga ggagctgaag aagcaagtag agaagctgca 7381 ggcccaggtc accctgacta atgcccagct caaaactctc aaagaggagg agaaggccaa 7441 ggaagccctc aaacagcaga agaggaaagc aaaggcttcg ggagagcgct accacatgga 7501 accccaccct gagcacgtct gcggcgccta tccctatgcc tacccaccca tgccagccat 7561 ggtacctcac catgcctaca aggactggtc ccagatccga taccctccac cccctgtgcc 7621 catggagcac ccgcccccac accccaactc tcgcctcttc catctgccgg agtacacctg 7681 gcgtccaccc tgtgcaggga ttcggaatca gagctctcaa gtgatggacc cgcccccaga 7741 caggcctgca gagccagagt ctgcagacaa tgactgtgat gggccccagt gaggctgcag 7801 tgggtcattt ggttccacct tcatctttca gagccagctg acctcagatt gccaaaagtt 7861 tgaaggccat gtgcatgttc tgtgtgaccc aagccttggc agaggagagg ctgggatggg 7921 tagctggctc acatccccag ccaagcctcg aactgttgac aagacccggg gatccgtcga 7981 cctgcagcca agctaattcc gggcgaattt cttatgattt atgattttta ttattaaata 8041 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa 8101 attcttgttc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga 8161 ggtcgctctt attgaccaca cctctaccgg catgcaagct tggcgtaatc atggtcatag 8221 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 8281 ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat tgcgttgcgc 8341 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tggattaatg aatcggccaa 8401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 8461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 8521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 8581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 8641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 8701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 8761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 8821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 8881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 8941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 9001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 9061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 9121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 9181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 9241 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 9301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 9361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 9421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 9481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 9541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 9601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 9661 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 9721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 9781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 9841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 9901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 9961 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 10021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 10081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 10141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 10201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 10261 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 10321 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct //